Difference between revisions of "User:Z5061522"

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[[User:Z8600021|Mark Hill]] ([[User talk:Z8600021|talk]]) 20:51, 24 April 2017 (AEST) This lab assessment will be marked by the guest presenter and the mark added here when I have received.
==Tutorial 1==  
==Tutorial 1==  

Revision as of 21:55, 25 April 2017

Welcome to Cell Biology 2017!

Lab 1 Assessment

Lab 2 Assessment

  1. Identify a chemical SDS and the risks and hazards of that chemical in text. Add a link to the original SDS
  2. Select 4 reference papers papers related to your selected group project topic sub-section. Read the research papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page.

Lab 3 Assessment - Endo/Exo worksheet questions.

Lab 4 Assessment

  1. Identify a cytoskeletal antibody.
  2. Identify the species deriving the antibody.
  3. Identify the working concentration for the antibody.
  4. Identify a secondary antibody that could be used with this antibody.
  5. Identify a paper that has used this antibody.

This assessment will be due by the next lab (Lab 5).

Lab 7 Assessment

The following peer assessment exercise should be completed before next lab (Lab 8 - 2 May) as your individual assessment for this week (lab missed due to public holiday).

Your answer should be pasted in 2 places

  1. onto each project discussion page (Note you should add anonymously to the discussion page)
  2. your own individual student page for my assessment.

Each individual will provide a brief assessment of the other groups projects. This should take the form of a brief critical (balanced) assessment identifying both the positive (good) and negative (bad) aspects of the project page as it currently exists online.

You may if you choose, use the final project assessment criteria as a guide. Though you are also welcome to use your own criteria.

Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.

Practical Attendance

Z5061522 (talk) 16:05, 7 March 2017 (AEDT)

Z5061522 (talk) 15:36, 14 March 2017 (AEDT)

Z5061522 (talk) 15:40, 28 March 2017 (AEDT)

Z5061522 (talk) 15:07, 4 April 2017 (AEST)

15:09, 11 April 2017 (AEST)

Individual Assessments

Lab 1

Silver-intensified cationic gold nanoparticle on cell membrane.png

Diagram of a cationic charged gold nanoparticle, with silver intensification, on a cell membrane.

Fogarty SW, Patel II, Martin FL, Fullwood NJ (2014) Surface-Enhanced Raman Spectroscopy of the Endothelial Cell Membrane. PLoS ONE 9(9): e106283. doi:10.1371/journal.pone.0106283

© 2014 Fogarty et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Lab 2

Part 1:



1. Flame (GHS02): Flammable

2. Exclamation Mark (GHS07): Acute Toxicity, Skin & Eye Irritant, Skin Sensitisation, Specific Target Organ Toxicity

3. Health Hazard (GHS08): Respiratory Sensitization, Germ Cell Mutagenicity, Carcinogenicity, Reproductive Toxicity, Specific Target Organ Toxicity, Aspiration Hazard

Website Address/Link: http://www.sigmaaldrich.com/catalog/product/sial/244511?lang=en&region=AU&cm_sp=Insite-_-prodRecCold_xviews-_-prodRecCold10-7

Part 2:

Article 1:

Article Title: Resveratrol and curcumin enhance pancreatic β-cell function by inhibiting phosphodiesterase activity

Article Description: The key aim of this study is to determine the relationship between pancreatic β-cell function, resveratrol (RES) and curcumin (CUR), to examine whether or not RES and CUR enhances pancreatic β-cell function. RES and CUR are polyphenols, found predominately within fruits and turmeric, which have been previously reported to contain medicinal properties that are beneficial to diabetes mellitus patients. Previously published studies claim that the therapeutic properties of RES and CUR can be attributed to their anti-inflammatory effects and protection against β-cell dysfunction. This study thus seeks to examine such reported phenomenon by examining the mechanism/s of action of RES and CUR in β-cells. Results indicate that RES and CUR regulate insulin secretion under glucose-stimulated conditions, and increase intracellular levels of cAMP, which plays an important role in insulin secretion and pancreatic β-cell health. The study also showcases that RES and CUR inhibit the activity of phosphodiesterases, which degrade cAMP. The polyphenols RES and CUR thus indeed enhance pancreatic β-cell function and have therapeutic benefits to diabetic patients.

Article Reference: <pubmed>25297556</pubmed>

Article 2:

Article Title: Ultrastructural Alterations of Pancreatic Beta Cells in Human Diabetes Mellitus

Article Description: The aim of this article is to investigate the correlation between pancreatic beta cells and diabetes mellitus, within humans. This is done by focusing on the ultrastructural alterations of beta cells in human diabetes. Throughout the study, beta cells have been retrieved from the pancreas of eight non-diabetic, five type 1 diabetic and eight type 2 diabetic organ donors, and analysed under morphometric electron microscopy. Results have indicated that lower quantities of beta cells are present in patients suffering type 1 and 2 diabetes, compared to non-diabetic patients. Additionally, insulin granules are more represented in non-diabetic patients than in type 2 diabetic patients, while type 1 diabetic patients show minimal changes. Pancreatic beta cells within diabetic patients have also showcased greater apoptosis than beta cells in non-diabetics. These results are expected to improve therapeutic measures for diabetes.

Article Reference: <pubmed>28303682</pubmed>

Article 3:

Article Title: Impairment of pancreatic β-cell function by chronic intermittent hypoxia

Article Description: The central purpose of this study is to demonstrate the link between chronic intermittent hypoxia (CIH) and pancreatic β-cell function. Via experimentation on rodents, the results of this study indicate that mitochondrial oxidative stress, which is produced by CIH, causes decreased pancreatic β-cell function, or increased pancreatic β-cell dysfunction. This is demonstrated by augmented basal insulin secretion, insulin resistance, defective proinsulin processing and impaired glucose-stimulated insulin secretion. This study thus contains direct evidence that CIH impacts β-cell function. As CIH is a hallmark of sleep apnoea, this study also indicates that CIH-impacted β-cell dysfunction could be a possible contributor to type 2 diabetes within sleep apnoea patients. It thus encourages possible future research in this area.

Article Reference: <pubmed>23709585</pubmed>

Article 4:

Article Title: Protein tyrosine phosphatase-1B modulates pancreatic β-cell mass

Article Description: The goal of this study is to analyse the action/s of protein tyrosine phosphatase 1B (PTP1B), which is an endoplasmic reticulum bound phosphatase that has been previously documented to negatively regulate insulin signalling, in pancreatic islets. Through experimental procedures on rodents and morphometric analysis of their pancreatic cells, the results of this study indicate that mice with PTP1B exhibit increased β-cell area, higher β-cell proliferation and decreased β-cell apoptosis, compared to their wild-type counterparts. This study thus sheds light on the involvement of PTP1B in β-cell physiology, and encourages the potential of PTP1B as a therapeutical target for the treatment of β-cell failure, which is characteristic of type 2 diabetes.

Article Reference: <pubmed>24587334</pubmed>

Lab 4

Antibody: beta Actin Polyclonal Antibody

Host/Isotope: Rabbit / IgG

Working Concentration: 1mg/ml

Secondary Antibody: Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555

Paper mentioning beta Actin Polyclonal Antibody: <pubmed>27001369</pubmed>

Lab 5

Lab assessment 5.PNG

Mark Hill (talk) 20:51, 24 April 2017 (AEST) This lab assessment will be marked by the guest presenter and the mark added here when I have received.

Tutorial 1


Human R3 RPTP members.png

Student Image Template

Reference Styles:

https://www.ncbi.nlm.nih.gov/pubmed or PubMed

Search Plasma Membrane

PMID: 28260084 NO LINK

PMID 28260084 LINK


Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.

Tutorial 2


Webpage: http://www.sigmaaldrich.com/catalog/product/sial/244511?lang=en&region=AU&cm_sp=Insite-_-prodRecCold_xviews-_-prodRecCold10-7

Biological Hazards of Toluene:

1. Flammable

2. Acute Toxicity; Skin Irritant, Eye Irritant

3. Respiratory Sensitization, Germ Cell Mutagenicity, Carcinogenicity, Reproductive Toxicity

Tutorial 4


Antibody: Monoclonal Anti-Cytokeratin

Host: Produced in mice

Isotype: IgG1

Species Reactivity: Bovine, frog, human, rat, kangaroo rat, mouse

Reference: http://www.sigmaaldrich.com/catalog/product/sigma/c2931?lang=en&region=AU

Secondary Antibody: PE anti-mouse IgG1

Secondary Antibody Description: The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b),

Reference: https://www.biolegend.com/en-us/products/pe-anti-mouse-igg1-6494

2017 Course Content


Lectures: Cell Biology Introduction | Cells Eukaryotes and Prokaryotes | Cell Membranes and Compartments | Cell Nucleus | Cell Export - Exocytosis | Cell Import - Endocytosis | Cytoskeleton Introduction | Cytoskeleton - Microfilaments | Cytoskeleton - Microtubules | Cytoskeleton - Intermediate Filaments | Cell Mitochondria | Cell Junctions | Extracellular Matrix 1 | Extracellular Matrix 2 | Cell Cycle | Cell Division | Cell Death 1 | Cell Death 2 | Signal 1 | Signal 2 | Stem Cells 1 | Stem Cells 2 | Development | 2017 Revision

2017 Laboratories: Introduction to Lab | Fixation and Staining |

2017 Projects: Group 1 - Delta | Group 2 - Duct | Group 3 - Beta | Group 4 - Alpha

Dr Mark Hill 2015, UNSW Cell Biology - UNSW CRICOS Provider Code No. 00098G