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Guilherme

--Z5050826 (talk) 16:34, 12 March 2015 (EST) Pubmed|

Cells_Eukaryotes_and_Prokaryotes

PMID 25513760


<pubmed>25513760</pubmed>

Lab Attendance

Lab 2--Z5050826 (talk) 16:53, 19 March 2015 (EST)

Lab 3--Z5050826 (talk) 17:04, 26 March 2015 (EST)

Lab 5--Z5050826 (talk) 16:41, 2 April 2015 (EST)

Lab 6--Z5050826 (talk) 17:38, 23 April 2015 (EST)

Lab 7 --Z5050826 (talk) 16:52, 30 April 2015 (EST)

Lab 8--Z5050826 (talk) 16:27, 7 May 2015 (EST)

Lab 9--Z5050826 (talk) 16:32, 14 May 2015 (EST)

Lab 11--Z5050826 (talk) 17:47, 21 May 2015 (EST)

Individual Assessments

Lab 1

Multiplecelldeath.jpg [1]

Multiple cell death http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688857/ <pubmed>PMC3688857</pubmed>

References

  1. <pubmed>PMC3688857</pubmed>

Lab 2

The paper discusses how Super Resolution Microscopy can be used in order to provide information about the synaptic active zone. The organization of the protein network of channels and receptors on the synaptic active zone is better understood when visualized in a nanometer scale, therefore the use of Super Resolution Microscopy is of great interest to the knowledge of molecular synaptic structure-function relationships.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311638/ <pubmed>PMC4311638</pubmed>

References

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311638/ <pubmed>PMC4311638</pubmed>

Paraformaldehyde

Paraformaldehyde MSDS

Lab 3

In silico analysis suggests interaction between Ebola virus and the extracellular matrix. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333865/ <pubmed>PMC4333865</pubmed>

Matrix metalloproteinases in destructive lung disease. http://www.sciencedirect.com/science/article/pii/S0945053X15000396

Thrombosis, like other cardiovascular diseases, has a strong genetic component, with largely unknown determinants. EMILIN2, Elastin Microfibril Interface Located Protein2, was identified as a candidate gene for thrombosis in mouse and human quantitative trait loci studies. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319747/ <pubmed>PMC4319747</pubmed>

Vitamin a deficiency and alterations in the extracellular matrix. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245576/ <pubmed>PMC4245576</pubmed>


Lab 5

Graficoguilherme.png

Lab 4

1. Monoclonal anti-elastin antibody

2. Monoclonal anti-elastin antibody produced in mouse

3. 25 µg/mL

4. Biotinylated chicken anti-human IgG antibody

5. Hong YJ, Kim J, Oh BR, et al. Serum Elastin-Derived Peptides and Anti-Elastin Antibody in Patients with Systemic Sclerosis. Journal of Korean Medical Science. 2012;27(5):484-488. doi:10.3346/jkms.2012.27.5.484. [1]

References

  1. <pubmed>PMC3342537</pubmed>

Lab 9

- Human cell line ATCC 90196B

Growth Medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%.

Iscove's modified Dulbecco's medium : ATCC

Formulation - Iscove's modified Dulbecco's medium

- Mouse cell line

Antibiotics Penicillin-Streptomycin

Groups Peer Review

Group 1

The project begins with an important and well-explained description about proteoglycans, informing that the group will focus on four particular types of proteoglycans due to its vast number. Thus, it is provided a brief history regarding proteoglycans, where you can learn about its discovery, the way it has been used for research overtime and the emerging evidence that some Small leucine-rich proteoglycans (SLRPs) can produce antitumorigenic effect, presenting an exciting new avenue for future researches.

Furthermore, the project covers glycosaminoglycans synthesis and SLRPs structure, supporting it with good evidence and pictures that allows a good understanding and a not boring approach.

Overall, decorin, biglycan, fibromodulin and lumican descriptions about structure, function and distribution are well-organized, though there is still some information missing regarding these proteoglycans in order to provide a better understanding about them. For instance, the function of lumina is not yet informed.

The role of SLRPs in disease: the project presents relevant diseases that are due to alterations in SLRPs, affecting different organs of the body. The explanations are clear, however there are still some images missing. Some editing is necessary in a way that the breaking down of long texts would turn the project even more interesting.

Group 2

The page is very well organized so far. However, there are some important information about future research that can be added, since interns are very relevant proteins with a lot of functions in the human body.

The structure section is still lacking some contents on synthesis and regulation, however so far the provided information is well-explained and easy to understand. The posted image, combined with the text, allows a clear comprehension on the general structure of the integrins.

Regarding the role of interns in disease, the association between cancer metastasis and the extracellular matrix is something that captures our attention due to cancer's prevalence and incidence. For this reason, I think it would be interesting to add some pictures to this section relating integrins and cancer, providing an even better understanding of this association.

Overall, I believe the page is well-organized so far, but some edit can still be made when regarding future researches, synthesis, regulations and clinical manifestations.

Group 4

The project presents a good introduction, history and structure explanations about fibronectin. However, I think the text is too long and lacks on understanding. It would be better understood if the information provided was more concise and some picture were added in order to break down the text.

The function section is also well-explained, giving different examples of fibronectin roles. Nonetheless, I believe there could be some more pictures on the page, in order to avoid a boring reading.

Regarding abnormalities/defects, I also believe that there is too much text and no images, making the page not to attractive for the readers.

Overall, the page is well constructed and organized, but I reckon some images could be added in a way the project is even more interesting.

Group 5

By having a general look on the page, I could say that the project is very disorganized. The references are separated according to each section, while they should be all in one at the end of the page.

Besides that, the page presents a wide list of laminas, making me ask if it is so important to talk about all of these instead of choosing the most important ones.

When reading the abnormalities section, it seems that the information is not clear enough to provide a fully understanding. I think there is too much text and lack of images, making it harder to comprehend.

The current research section is certainly of great importance, but I reckon the text should be summarized in order to clarify its message and broke down with images so that the page looks more interesting and attractive.

Overall, the page has the necessary content. However, some editing is still necessary in regards of organization and important information.

Group 6

By having an overall look on group's 6 project page, I can see that the topics are all included, but some information still lacks. Type II Collagen is a very important molecule with a wide range of functions. For this reason, I think that the abnormalities section should be more complete, with some pictures helping the understanding. I also think that Kniest Dysplasia and Rheumatoid Arthritis could be better explained, in a way we can comprehend them better and relate to Type II Collagen more easily.

Overall, so far the project is well-developed, but not completed. Some editing with the addition of pictures and some missing content is necessary. Good job so far, though! :)

Group 7

This page is on Basement Membrane, a very relevant structure for epithelium and other cells. So far, the page is very well organized. I think that there is an important use of images on this page, making it easy to understand the introduction, formation and functions of the Basement Membrane. The structural components section, although it has no pictures, is well-explained with a concise text, making it easy to understand and not boring to read. The referencing is also well-done.

On the abnormalities section, I liked the way additional information about glomerulus and rare abnormalities were performed, giving to the reader the option of expand or collapse. This is a good manner to organize some extra information. Furthermore, I reckon the abnormalities have a good description and the provided images facilitate the complete understanding of the clinical manifestations.

Overall, I think group 7 is making an excellent job so far. Perhaps adding a video could help the reader have an even better comprehension.