User:Z5050826

From CellBiology

Guilherme

--Z5050826 (talk) 16:34, 12 March 2015 (EST) Pubmed|

Cells_Eukaryotes_and_Prokaryotes

PMID 25513760


<pubmed>25513760</pubmed>

Lab Attendance

Lab 2--Z5050826 (talk) 16:53, 19 March 2015 (EST)

Lab 3--Z5050826 (talk) 17:04, 26 March 2015 (EST)

Lab 5--Z5050826 (talk) 16:41, 2 April 2015 (EST)

Lab 6--Z5050826 (talk) 17:38, 23 April 2015 (EST)

Lab 7 --Z5050826 (talk) 16:52, 30 April 2015 (EST)

Lab 8--Z5050826 (talk) 16:27, 7 May 2015 (EST)

Lab 9--Z5050826 (talk) 16:32, 14 May 2015 (EST)

Individual Assessments

Lab 1

Multiplecelldeath.jpg [1]

Multiple cell death http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688857/ <pubmed>PMC3688857</pubmed>

References

  1. <pubmed>PMC3688857</pubmed>

Lab 2

The paper discusses how Super Resolution Microscopy can be used in order to provide information about the synaptic active zone. The organization of the protein network of channels and receptors on the synaptic active zone is better understood when visualized in a nanometer scale, therefore the use of Super Resolution Microscopy is of great interest to the knowledge of molecular synaptic structure-function relationships.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311638/ <pubmed>PMC4311638</pubmed>

References

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311638/ <pubmed>PMC4311638</pubmed>

Paraformaldehyde

Paraformaldehyde MSDS

Lab 3

In silico analysis suggests interaction between Ebola virus and the extracellular matrix. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333865/ <pubmed>PMC4333865</pubmed>

Matrix metalloproteinases in destructive lung disease. http://www.sciencedirect.com/science/article/pii/S0945053X15000396

Thrombosis, like other cardiovascular diseases, has a strong genetic component, with largely unknown determinants. EMILIN2, Elastin Microfibril Interface Located Protein2, was identified as a candidate gene for thrombosis in mouse and human quantitative trait loci studies. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319747/ <pubmed>PMC4319747</pubmed>

Vitamin a deficiency and alterations in the extracellular matrix. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245576/ <pubmed>PMC4245576</pubmed>


Lab 5

Graficoguilherme.png

Lab 4

1. Monoclonal anti-elastin antibody

2. Monoclonal anti-elastin antibody produced in mouse

3. 25 µg/mL

4. Biotinylated chicken anti-human IgG antibody

5. Hong YJ, Kim J, Oh BR, et al. Serum Elastin-Derived Peptides and Anti-Elastin Antibody in Patients with Systemic Sclerosis. Journal of Korean Medical Science. 2012;27(5):484-488. doi:10.3346/jkms.2012.27.5.484. [1]

References

  1. <pubmed>PMC3342537</pubmed>

Lab 9

- Human cell line ATCC 90196B

Growth Medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%.

Iscove's modified Dulbecco's medium : ATCC

Formulation - Iscove's modified Dulbecco's medium

- Mouse cell line

Antibiotics Penicillin-Streptomycin