Anti-HA Stained HeLa Cells
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Anti-HA Stained HeLa Cells
Lab 2 Super-Microscopy Summary
Optical diffraction is a limiting factor of resolution quality in previous light microscopy methods. Zeng and collaborators compared the efficacy of joint tagging super-resolution optical fluctuation imaging(JT-SOFI) and super-resolution optical fluctuation imaging(SOFI) on creating super-resolution images. Through the implementation of blinking quantum dots in combination with different labelling densities, SOFI images can be compared testing the visibility of stained biological components, microtubule networks in this case. Results concluded joint tagging maintained visibility over the original single tagging scheme found in previous SOFI methods. JT-SOFI additionally decreased the frame number to 100, allowing molecular components to be discernible, as well as, produce high density labelling in conjunction with maintaining continuities of biological structures.
Group 7 Topic: Basement Membrane
Structure: (4 articles)
<pubmed>PMC3700973</pubmed> Brief Summary: Basement membranes(BM) have bi-functional organization. Each face of the BM has side-specific properties that determine the adherence of epithelial and connective tissues. Mice and chick embryo models confirmed the side-specific properties of the BM.
Project Information: The BM has morphological differences between the epithelial and stromal sides. The epithelial side faces outward and the stromal side inward. The BM epithelial side adheres to epithelial cells and exhibits high levels of laminin proteins, whereas the stromal side adheres to connective tissue cells and exhibits high levels of collagen IV.
<pubmed>PMC3526973</pubmed> Brief Summary: The basement membrane acts as a malignancy suppressor, with matrix proteins that form a barrier to pre-invasive lesions. This membrane regenerates and remodels itself in an adult organism, thus, can become structurally compromised if degradation of the extracellular matrix proteins occurs. Potential roles of self-generated forces in adult tissue remodeling are discussed in this article.
Project Information: Structural integrity, plasticity, and regeneration of the basement membrane are important components to note in the structural information of this project. The basement membrane is not a static structure. It is constantly regenerated creating different ratios of ECM proteins over the life of an organism.
<pubmed>PMC3969375</pubmed> Basic Summary: The basement membrane and the extracellular matrix are structurally modified during acute ischemic injury. Structural changes in collagen-IV-fragments of the basement membrane were detected in a large animal model of ischemia-reperfusion.
Project Information: This article gives information about the two structural components of basal lamina, collagen IV and perlecan, and their functions with the extracellular matrix and surrounding cells.
<pubmed>18834439</pubmed> Brief Summary: The findings of this article reveal the differences between the basement membrane structure of human and rodent pancreatic islet cells. Rodent Beta cells interact directly with basement membranes of capillary endothelial cells while basement membranes in human islets are separate from the endothelia. This article gives information about basement membrane structures and functions.
Project Information: Type IV collagen and laminin are the major structural components of basement membranes. These components are connected by nidogen and other minor components.
Transmission electron micrograph of rat kidney filtration barrier illustrating the basement membrane between fenestrated capillary endothelium and podocyte foot processes
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License.
- Note - This image was originally uploaded as part of a student project and may contain inaccuracies in either description or acknowledgements. Please contact the site coordinator if the uploaded content does not meet the original copyright permission or requirements, for immediate removal.
1. Identify an antibody that can been used in your group's extracellular matrix project: Anti-Laminin antibody [LAM-89]
2.Identify the species deriving the antibody: Mouse monoclonal [LAM-89] to Laminin
3. Identify the working concentration for the antibody: 0.5 µg/ml
4.Identify a secondary antibody that could be used with this antibody: Anti-Laminin antibody, Rabbit polyclonal to Laminin
5.Identify a paper that has used this antibody:
Growth Medium: ATCC-formulated Dulbecco's Modified Eagle's medium
Contains: 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate
Growth Medium: ATCC-formulated Dulbecco's Modified Eagle's Medium
Contains: 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.
Mode of Action: Penicillin-Streptomycin acts in two ways. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.
Project 1-6 Assessments
Project 1: Proteoglycans
This is a good starting point for your Proteoglycan web page. There is evidence of research in each topic of discussion, however, not everything is clearly described. The Early Research and Advances in Technology and Discovery sections display good background information. My tip would be to discuss what GAG's are in structure and function since you have found that there is substantial information on them. Also, adding in SLRP synthesis might be beneficial. When mentioning specific scientific terms that are important to the knowledge of proteoglycans, such as leucine and TFG-B1, give more information. It's better to break down the information so that it is more easily understood by student readers.
I liked the diagrams and photos that you used throughout the webpage. They give the reader a better visual understanding of your topic. Adding in more will only benefit your page. Some paragraphs are choppy and need revision. There are multiple grammatical errors, run-on sentences, and problems with verb tense. Reading aloud will help to pick out mistakes and make changes so that the paragraphs and sentences flow.
The Structure and Function sections need the most work done on them. When discussing secreted proteins in the Structure portion of your web page, I was confused on what that information meant. However, I'm sure you were already planning on expanding on multiple topics that weren't fully described yet. I enjoyed the role of SLRPs in disease section the most. It gave a lot of good information and I appreciated the use of photographs. Grammatical revision will make this section even better.
Project 2: Integrins
Overall, I thought this was a very strong web page. There was a good amount of in-depth information on your topic and most of it was well-written. The Structure section was well analysed and the photo provided a good visual aid. I am interested in understanding the rest of integrin specificity when it is finished. I thought the video was a good addition, however, it moved through the process very quickly and the words weren't up on the screen long enough to read. I had to re-watch it to see what was written. Plus, there was no sound. I'm not sure if that was intentional or it could just be my computer. Nevertheless, I think sound would be beneficial.
There were grammatical errors throughout the webpage, as well as, issues with sentence structure. Re-reading through the webpage would be beneficial. Also, there were multiple times that the word, integrin, was capitalized where it was not the beginning of the sentence. I do not think that capitalization is needed in these cases.
Additional info on integrin relationships with fibronectin, collagen, and laminin would be beneficial for the reader to gain a better understanding about the functions of integrins with other ECM components. The Role of Integrins in Disease section was well-written and gave significant information about each role of integrins. This section was the strongest in my opinion.
Project 3: Elastic Fibres
Your webpage has many positive aspects to it. I thought the organization of the material on the page was great. You also made the material easily understood for readers without prior knowledge of the subject. I thought the rubber band analogy was a good idea to include in your webpage as it gave a good mental picture for the reader, making the subject relatable. It was evident that thorough research of the subject matter was conducted.
To make your webpage better, I would suggest going through and re-reading for grammatical errors as there were some run-on sentences as well as issues with sentence structure. There were some points where phrases were re-used multiple times within a few sentences. For example, 'of elastin' became repetitive within the Tropomyosin and Elastin section.
I appreciated the hand drawn image you created. It seemed very informative. However, the words in the key along with the labels were not legible. With correction of this issue, the image would be fantastic to incorporate. I thought you included a good ratio of photos to text within your webpage. There were photos in the Heart and Lungs sections that needed legends. Otherwise, I thought your project was well-written and consistent in information depth throughout.
Project 4: Fibronectin
I really enjoyed this webpage. Your work was neat in organization which gave the information a good, progressive flow. The structure section was well put together and there is evidence of detailed analysis of the information provided. The chronological structure of information aided in reader comprehension of this subject.
Though the text was laid out well, there was a lack of pictures, video and other media tools present to help with reader understanding. Much of the text became tedious to read due to the lack of visual aids within the webpage. The photos that were included need a legend of some sort explaining what they are and how they relate to the information in the text. Including more media within the introduction, structure, function, and abnormalities sections would give visual interest to your webpage.
Overall, I thought I gained a good understanding of what fibronectin is structurally and how it functions within the body. The only questions I have are, "Are fibronectin structures uniform in every cell within the body?", and, "Do all cells possess the same amount of fibronectin, or is there variation?". These questions may provide useful information to include in your webpage. Nevertheless, you have done a great job!
Project 5: Laminin
Overall, the webpage is very informative. It is evident that significant research was conducted on this topic in all areas of discussion. Initially, I became overwhelmed with the amount of text on the page. There was a lack of interesting visual aids, photos and other types of media, to keep the webpage interesting. Also, due to the large amount of information written in paragraphs, reading became tedious. For the history section, it may be best to break up the paragraphs into bullet points which include only the most significant information. Changing up the structure of which the information is presented will give your page visual interest.
I also noticed the references listed at the end of each section. These should all go at the end of the webpage so that all references are presented together. Re-reading for grammatical errors will also make the page more professional.
I liked the images that you have included on the page, however, the photo in the structure section should be located directly beside the information that it is referencing for easy viewing, rather than below the text. In addition to this, the Image section does not seem appropriate for the webpage. I think that you were probably going to change this, but anyway, the copyright should also not be located directly on this page. It should be embedded within the photo, so that when you click on the photo, the copyright will be listed along with the original figure legend. This photo should also be accompanied by text that is not the legend, providing a reason for the use of the photo.
I really liked that the many forms of laminin were included in the page along with their functions. This information did seem overwhelming as a list, so putting the listed forms of laminin into a pop-up table would be a perfect addition to your page. This would also be useful in providing a reference for when you specifically talk about these laminin types in the abnormalities section. You webpage is on the right track! I really appreciated all the time put into providing detailed information. This is definitely the strong point in your work.
Project 6: Type II Collagen
This is a solid start to your project. There is good information described in all sections currently filled out. There are other sections along with stated questions that need to be filled out and answered. You probably were already planning on doing that. Also, re-reading through your current work to check for grammatical mistakes will make your webpage more professional.
I really liked the photo you incorporated in the function section. It clearly illustrates the cartilage zones which helps the reader to visualize the information you have discussed. Adding more photos and other sorts of media into your webpage will balance out the text you incorporate on your page, as well as, make the page more interesting.
I think you have a good start to your function and abnormalities sections. However, it seems there is still a good bit of information needed to fill out all of the holes in your work, especially in the abnormalities section. Just a few tips: When adding in additional information, also think about making the page visually interesting by breaking up the information with visuals that aid the reader in comprehension. Also, I liked that you included an antibodies section. However, when adding in significant amounts of information, this info might work best as a link to the page where this info can be found as this is supplementary to your topic. Best of luck with your editing!
This research article uses the comparison of tissue microarray results from cervical cancer and normal cervix tissue samples to investigate the role of normal and tumor-associated fibroblasts. The microarray results revealed an increased reorganization of the fibrillar stroma of these tumors and further testing concluded an increase in laminin-1 in CSCC7 cells.