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This is about Prokaryote.
Chlamydomonas cells in neon
- <pubmed>20076674</pubmed>| PLoS One.
Individual Assessment - Lab 2
Hutchinson-Gilford Progeria Syndrome and nucleus
- <pubmed>16277559</pubmed>|PLoS One.
Stimulates Emission Depletion (STED) microscopy is a super-resolution microscopy that was used to measure the size of mitochondrial nucleoids. An important feature of this device was that it enabled a resolution of 40 to 50 nm and thus could give a reliable picture of the nucleoid diameter (~100nm - antibody decorated).This form of microscopy is advantageous as no tissue sectioning is necessary which allows the capacity to produce a more realistic image. Using STED microscopy, mitochondrial nucleoids from several mammalian species were analysed and the results showed that all mitochondrial nucleoids had uniform sizes and were often organized in clusters. It was also observed that there was only a single copy of mtDNA present within most mitochondria nucleoids.
Individual Assessment - Lab 3
I went for a search after old articles that wrote about Nuclear Pore Complex (NPC) so I could grasp some simple concepts about it. “Architecture and Design of Nuclear Pore Complex” talks about its structure and they figured the approximate diameter of the nuclear pore by coating cargo-receptor with gold particles. Division of the nuclear pore into Peripheral and Central channels was also noticed by observing dissected macronuclei from Xenopus laevis oocytes on electron microscopy. In addition, the article tells you about the role these channels play in passive and active transports through nuclear membrane, as well as a picture of their structure.
This article explore the wide role of Nuclear Pore Complex (NPC) on passive and facilitated transports, once this is the only route material can go from nucleus to cytoplasm and vice-versa. The experiment used HeLa cells, where marked molecules were injected. The range of molecules included ones that could go through NPC either by passive diffusion or receptor-mediated routes. It was shown that ions, metabolites and intermediated-sized macromolecules crossed the nuclear membrane by diffusion, whereas proteins, RNA and ribonucleoprotein complexes needed the facilitated transport. It was noticed that though both transports occurred in the central part of NPC they didn't interfere with each other. However, molecules utilizing the same mode of transport affected each other pathways. Hence it was concluded that passive and facilitated transports don't share the same route.