Difference between revisions of "User:Z5011434"

From CellBiology
Line 52: Line 52:
  
 
Stimulates Emission Depletion (STED) microscopy is a super-resolution microscopy that was used to measure the size of mitochondrial nucleoids. An important feature of this device was that it enabled a resolution of 40 to 50 nm and thus could give a reliable picture of the nucleoid diameter (~100nm - antibody decorated).This form of microscopy is advantageous as no tissue sectioning is necessary which allows the capacity to produce a more realistic image. Using STED microscopy, mitochondrial nucleoids from several mammalian species were analysed and the results showed that all mitochondrial nucleoids had uniform sizes and were often organized in clusters. It was also observed that there was only a single copy of mtDNA present within most mitochondria nucleoids.<ref><pubmed>21808029</pubmed></ref>
 
Stimulates Emission Depletion (STED) microscopy is a super-resolution microscopy that was used to measure the size of mitochondrial nucleoids. An important feature of this device was that it enabled a resolution of 40 to 50 nm and thus could give a reliable picture of the nucleoid diameter (~100nm - antibody decorated).This form of microscopy is advantageous as no tissue sectioning is necessary which allows the capacity to produce a more realistic image. Using STED microscopy, mitochondrial nucleoids from several mammalian species were analysed and the results showed that all mitochondrial nucleoids had uniform sizes and were often organized in clusters. It was also observed that there was only a single copy of mtDNA present within most mitochondria nucleoids.<ref><pubmed>21808029</pubmed></ref>
 +
 +
<references/>
 +
 +
==Individual Assessmet - Lab 3==
 +
 +
===Part 1===
 +
 +
I went for a search after old articles that wrote about Nuclear Pore Complex (NPC) so I could grasp some simple concepts about it. “Architecture and Design of Nuclear Pore Complex” talks about its structure and they figured the approximate diameter of the nuclear pore by coating cargo-receptor with gold particles. Division of the nuclear pore into Peripheral and Central channels was also noticed by observing dissected macronuclei from Xenopus laevis oocytes on electron microscopy. In addition, the article tells you about the role these channels play in passive and active transports through nuclear membrane, as well as a picture of their structure.<ref><pubmed>1617726</pubmed></ref>
  
 
<references/>
 
<references/>

Revision as of 00:24, 3 April 2014

Add your own student page to the site.

Add your signature for Lab attendance.

--Z5011434 (talk) 15:47, 13 March 2014 (EST)

--Z5011434 (talk) 15:07, 20 March 2014 (EST)

--Z5011434 (talk) 15:12, 27 March 2014 (EST)

Add a sub-heading

Add an external Link

PubMed

Add an internal Link

Laboratory 1

beutiful

Reference

This is about Prokaryote.[1]

<pubmed limit=5>Prokaryote</pubmed>

  1. <pubmed>24603758</pubmed>


Test Image

Chlamydomonas cells in neon.png

Chlamydomonas cells in neon[1]

  1. <pubmed>20076674</pubmed>| PLoS One.

Individual Assessment - Lab 2

Part 1

Progeria Syndrome.png

Hutchinson-Gilford Progeria Syndrome and nucleus[1]

  1. <pubmed>16277559</pubmed>|PLoS One.

Part 2

Stimulates Emission Depletion (STED) microscopy is a super-resolution microscopy that was used to measure the size of mitochondrial nucleoids. An important feature of this device was that it enabled a resolution of 40 to 50 nm and thus could give a reliable picture of the nucleoid diameter (~100nm - antibody decorated).This form of microscopy is advantageous as no tissue sectioning is necessary which allows the capacity to produce a more realistic image. Using STED microscopy, mitochondrial nucleoids from several mammalian species were analysed and the results showed that all mitochondrial nucleoids had uniform sizes and were often organized in clusters. It was also observed that there was only a single copy of mtDNA present within most mitochondria nucleoids.[1]

  1. <pubmed>21808029</pubmed>

Individual Assessmet - Lab 3

Part 1

I went for a search after old articles that wrote about Nuclear Pore Complex (NPC) so I could grasp some simple concepts about it. “Architecture and Design of Nuclear Pore Complex” talks about its structure and they figured the approximate diameter of the nuclear pore by coating cargo-receptor with gold particles. Division of the nuclear pore into Peripheral and Central channels was also noticed by observing dissected macronuclei from Xenopus laevis oocytes on electron microscopy. In addition, the article tells you about the role these channels play in passive and active transports through nuclear membrane, as well as a picture of their structure.[1]

  1. <pubmed>1617726</pubmed>