Difference between revisions of "User:Z5011434"

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===Part 2===
 
===Part 2===
  
Stimulates Emission Depletion (STED) microscopy is a super-resolution microscopy that is used to measure the size of mitochondrial nucleoids. An important feature of this device was that it enabled a resolution of 40 to 50 nm and thus could give a reliable picture of the nucleoid diameter (~100nm - antibody decorated).This form of microscopy is advantageous as no tissue sectioning is necessary which allows the capacity to produce a more realistic image. Using STED microscopy, mitochondrial nucleoids from several mammalian species were analysed and the results showed that all mitochondrial nucleoids had uniform sizes and were often organized in clusters. It was also observed that there was only a single copy of mtDNA present within most mitochondria nucleoids
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Stimulates Emission Depletion (STED) microscopy is a super-resolution microscopy that is used to measure the size of mitochondrial nucleoids. An important feature of this device was that it enabled a resolution of 40 to 50 nm and thus could give a reliable picture of the nucleoid diameter (~100nm - antibody decorated).This form of microscopy is advantageous as no tissue sectioning is necessary which allows the capacity to produce a more realistic image. Using STED microscopy, mitochondrial nucleoids from several mammalian species were analysed and the results showed that all mitochondrial nucleoids had uniform sizes and were often organized in clusters. It was also observed that there was only a single copy of mtDNA present within most mitochondria nucleoids.
  
 
[http://www.pnas.org/content/108/33/13534.full Super-resolution microscopy and mitochondrial nucleoids]
 
[http://www.pnas.org/content/108/33/13534.full Super-resolution microscopy and mitochondrial nucleoids]

Revision as of 20:40, 26 March 2014

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Laboratory 1

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Reference

This is about Prokaryote.[1]

<pubmed limit=5>Prokaryote</pubmed>

  1. <pubmed>24603758</pubmed>


Test Image

Chlamydomonas cells in neon.png

Chlamydomonas cells in neon[1]

  1. <pubmed>20076674</pubmed>| PLoS One.

Individual Assessment - Lab 2

Part 1

Progeria Syndrome.png

Hutchinson-Gilford Progeria Syndrome and nucleus[1]

  1. <pubmed>16277559</pubmed>|PLoS One.

Part 2

Stimulates Emission Depletion (STED) microscopy is a super-resolution microscopy that is used to measure the size of mitochondrial nucleoids. An important feature of this device was that it enabled a resolution of 40 to 50 nm and thus could give a reliable picture of the nucleoid diameter (~100nm - antibody decorated).This form of microscopy is advantageous as no tissue sectioning is necessary which allows the capacity to produce a more realistic image. Using STED microscopy, mitochondrial nucleoids from several mammalian species were analysed and the results showed that all mitochondrial nucleoids had uniform sizes and were often organized in clusters. It was also observed that there was only a single copy of mtDNA present within most mitochondria nucleoids.

Super-resolution microscopy and mitochondrial nucleoids