User:Z3418837

From CellBiology
Revision as of 14:13, 27 March 2014 by Z3418837 (talk | contribs)

Add your own student page to the site.

Add your signature for Lab attendance.

--Z3418837 (talk) 15:45, 13 March 2014 (EST)

--Z3418837 (talk) 15:07, 20 March 2014 (EST)

--Z3418837 (talk) 15:13, 27 March 2014 (EST)

Add a sub-heading.

Add an external Link.

PubMed

Add an internal Link.

Mickey Mouse

This is a wonderful picture of two cells.

Reference

<pubmed>24603758</pubmed>

This is about Prokaryotes.[1]

<pubmed limit=5>Prokaryote</pubmed>


  1. <pubmed>24603758</pubmed>


Individual Assessments

Lab 1

Chloroplast present in Eukaryotes and not in Prokaryotes.png

Chloroplast present in Eukaryotes and not in Prokaryotes [1]

  1. <pubmed>17710148</pubmed>| PLoS One.

Copyright: © 2007 Henderson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

--Z3418837 (talk) 21:05, 18 March 2014 (EST)

Lab 2

1. On your own student page upload an image with the reference using the Pubmed formatting shown in the practical class tutorial last week.

Plant Nucleus.png

Plant Nucleus [1]

  1. <pubmed>23155403</pubmed>| PLoS One.

Copyright © 2012 Gao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

--Z3418837 (talk) 21:42, 23 March 2014 (EST)


2. Identify a recent research article (not review) that uses either confocal microscopy or super-resolution microscopy as one of the study's techniques. Explain briefly (1 paragraph) how the microscopy technique specifically contributed to the article's findings.

In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach.

The purpose of the study was to measure the intracellular pH of organelles in situ using the physics behind confocal microscopy. This was achieved by extracting the colonies ofSaccharomyces cerevisiae which were associated at various developmental stages. Confocal fluorescence microscopy was then used to monitor the variation in intracellular pH by targeting fusion proteins to subcellular compartments and then quantifying the fluctuations in the emission wavelengths obtained. The use of confocal microscopy imaging enabled researchers to distinguish between the various fluorescent fractions that were present in single cells. It also provided enhanced readings of emissions by the decreased photobleaching and amplified signal-to-noise ratios which detected a wide range of fusion proteins and intracellular pKa levels leading to precise quantifications of organellar pH. Ultimately, the use of confocal microscopy imaging has advanced to such a high level that determining intracellular level of pH is simple and efficient compared to the previous methods of microscopy and spectrofluorometry used. Confocal microscopy also promises much greater result as research proceeds.

<pubmed>22470445</pubmed>

--Z3418837 (talk) 23:45, 23 March 2014 (EST)