From CellBiology


Group 2 Project page[1]

Lab Attendance

--Z3415735 (talk) 16:35, 12 March 2015 (EST) --Z3415735 (talk) 16:54, 19 March 2015 (EST) --Z3415735 (talk) 17:27, 26 March 2015 (EST) --Z3415735 (talk) 16:42, 30 April 2015 (EST) --Z3415735 (talk) 17:06, 7 May 2015 (EST)

Lab 1

Interconnections between S. Aureus and platelets



Lab 2

The following article refers to a research study published in March, 2015. Research was focused around investigation of the cellular processes that contribute to the regulation of osmosis in mammalian cells. More specifically, they investigated the "swelling-activated pathways" for myo-inositol (a significant osmolyte i.e. a substance that influences osmotic rate) and how certain protein transporters may influence the transport of myo-inositol. Their data showed that swelling-activated proteins (SLC5A3) were substantially influential in the regulation of osmosis via transporting mechanisms.

Super-resolution microscopy was essential for the investigation of the cellular interactions present on the cell cultures grown in the laboratory. dSTORM was the specific method of Super-resolution microscopy that was used. This kind of microscopy falls under the category of "Stochastic super-resolution" microscopy.

Article reference:[2]

Additional information:[3]

Lab 3

Paraformaldehyde MSDS[4]

Integrin Structure and Function schematic.jpg

Structure of Integrins

<pubmed>25606594</pubmed> This article discusses the influence of Integrins in the development of mammary tissue, and particular, its role in the co-functioning with the ECM.

<pubmed>25837254</pubmed> Integrins are involved in the identification of RGD motifs, which are essential for ECM function. The article discusses the clincal relevance of this in relation to Helicobacter Pylori infection in the stomach.

<pubmed>25754646</pubmed> This article discusses how Talin (an intracellular protein) is an important factor in the linkage of Integrins and ECM to the actin cytoskeleton.

<pubmed>25368556</pubmed> Discussion of how Integrins (and other proteins) interact with ECM to influence synaptic plasticity in neurons.

Lab 9 Mammary gland epithelium. Mouse. Female

Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10% Osteosarcoma cell line. Human. 13 years old, Female. Caucasian

Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%


PubMed [2]

Lecture 1 [3]