Difference between revisions of "User:Z3399239"

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==Lab 8==
==Lab 8==
--[[User:Z3399239|Z3399239]] ([[User talk:Z3399239|talk]]) 12:41, 15 May 2014 (EST)
--[[User:Z3399239|Z3399239]] ([[User talk:Z3399239|talk]]) 12:42, 15 May 2014 (EST)
Group 2 Peer-Review
Group 2 Peer-Review

Revision as of 13:42, 15 May 2014

   Add your own student page to the site.

   Add your signature for Lab attendance.

--Z3399239 (talk) 15:45, 13 March 2014 (EST)

   Add a sub-heading.


   Add an external Link.

z3373930 z3420257

   Add an internal Link.

Lab 1

Cell Biology

Sodium Sulfate[1]

<pubmed limit=5>Sodium sulfate</pubmed>


  1. <pubmed>24604303</pubmed>

Individual Assessments

Lab 1

Bacillus Cell Membrane




Copyright: © 2011 Dempwolff et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

--Z3399239 (talk) 15:07, 20 March 2014 (EST)

Lab 2

Nuclear membrane.png

Image of Nuclear membranes




© 2009 Garnier-Lhomme et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

--Z3399239 (talk) 19:04, 20 March 2014 (EST)

Super-Resolution Microscopy Strategies in Cell Biology Using a Spinning Disk Microscope

Hosny N.A. et al. (2013) present a comparative study of super-resolution microscopy strategies using two methods of super-resolution microscopy; Photoactivation Light-Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM), in conjunction with Spinning disk super-resolution imaging (SDSI) or Structured Illumination microscopy (SIM) and differing image analysis algorithms (RainSTORM, QuickPALM, GLRT, SOFI, 3B, Deconvolution-STORM (DeconSTORM), and Faster-STORM). Standard confocal microscopy has a resolution limit of 200nm, which has prevented further research into small molecular structures e.g. nuclear ultrastructure. Super-resolution microscopy has bypassed this resolution limit (described by Abbe's Law) and allowed for observation of structures as small as 30nm in size.

The study suggests that; 1) Multi-spectral SDSI can collect super-resolution images with good signal-to-noise (S/N), resolved in any selected axial plane within a cell. 2) PALM and STORM can both be used separately or in conjunction to produce super-resolution data. 3) SOFI has the best retention of image intensity information and provides the most accurate data reconstruction, in terms of spatially assigning all of the emission data found in the original images. 4) SIM was more appropriate for imaging 3D structures. 5) PALM/STORM SDSI could generate higher resolved data than SIM for single plane imaging dependent on the image processing algorithm used



--Z3399239 (talk) 12:13, 27 March 2014 (EST)

--Mark Hill (talk) 14:30, 3 April 2014 (EST) Good.

Lab 3

Research on trans-endocytosis; Trans-endocytosis is a process whereby material created in one cell is incorporated into another cell through endocytosis.

Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47–SHPS-1 system

<pubmed>18349073</pubmed> This article looks at trans-endocytosis of transmembrane proteins CD47 and SHPS-1. The study suggests that CD47 and SHPS-1 interaction initiates the transfer of CD47 from CD47-expressing cells to neighboring SHPS-1-expressing cells followed by the internalization of the ligand-receptor complex into the SHPS-1-expressing cells. SHPS-1 was found to undergo trans-endocytosis from SHPS-1-expressing cells to neighboring CD47-expressing cells, suggesting that trans-endocytosis of CD47 and SHPS-1 occurs bidirectionally. The study suggests that CD47 trans-endocytosis is implicated in the regulation of the CD47–SHPS-1 system.

This article is relevant to the sub-topic of trans-endocytosis as it describes the mechanism and physiological roles of endocytosis and gives a specific example of trans membrane protein trans-endocytosis.

Trans-Endocytosis of CD80 and CD86: A Molecular Basis for the Cell-Extrinsic Function of CTLA-4


Qureshi et al. (2011) investigate the cell-extrinsic mechanisms of Cytotoxic T lymphocyte antigen 4 (CTLA-4). CTLA-4 is thought to be an important factor in the prevention of autoimmune disease. Trans-endocytosis of CD80 and CD86 (ligands shared by both CLTA-4 and CD28, a stimulatory receptor) inhibits co-stimulation of CD28. The study suggests trans-endocytosis as a possible cell-extrinsic model of CLTA-4 function whereby co-stimulatory ligands are removed from antigen presenting cells (APCs).

This study is relevant as it outlines a mechanism whereby trans-endocytosis is used to inhibit co-stimulation of another cell. The study also shows that trans-endocytosis can play a regulatory role in autoimmune diseases.

β-Arrestin1 Mediates the Endocytosis and Functions of Macrophage Migration Inhibitory Factor


Xie et al. (2011) investigate the effects of Macrophage migration inhibitory factor (MIF) and β-Arrestin on MIF endocytosis. The study suggests that MIF utilises β-arrestin1 as a molecular scaffold to maintain integrity and specificity of signalling.

This study relates endocytosis to cytokines and chemical mediators involved regulating inflammatory and immune responses.

Uptake of MIF via CPZ sensitive endocytosis.png

Image of Uptake of MIF via CPZ sensitive endocytosis




© 2011 Xie et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Jak2 is a negative regulator of ubiquitin-dependent endocytosis of the growth hormone receptor


Putters et al. (2011) investigate the ability of Janus kinase 2 (Jak2) to bind to the growth hormone receptor, preventing endocytosis of growth hormone receptor (GHR). The article shows that Jak2 specifically inhibits GHR endocytosis independent of its kinase activity and Growth Hormone-induced and constitutive endocytosis undergo the same mechanism of endocytosis.

This article is relevant because it shows that both growth hormone-induced and constitutive growth hormone receptor endocytosis depend on the same factors, therefore strongly suggesting that the modes of endocytosis are similar, if not identical.

Lab 4

--Z3399239 (talk) 15:11, 3 April 2014 (EST)

   Identify an antibody against an adhesion junction protein that is commercially available.

Anti-E Cadherin [HECD-1] antibody

   Add a link to the original data sheet page and identify the type of adhesion junction.

[1] - Tight junctions (present in all cells but most prevalent in epithelial cells and also forms the blood brain barrier)

   Include the following information: type of antibody (polyclonal, monoclonal), species raised in, species reacts against, types of application uses, and if available any reference using that antibody. 

Type of Antibody: Monoclonal

Species raised in: Raised in humans

Species reacts against: Does not react with Mouse or Rat

Types of Application: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry, Immunohistochemistry (frozen sections), Immunocytochemistry/Immunoflourescence, Western Blot

References: <pubmed>23584474</pubmed> <pubmed>23752180</pubmed> <pubmed>23825587</pubmed>

--Z3399239 (talk) 11:31, 10 April 2014 (EST)

[2] - Junctional Adhesion Molecule 1(present in tight junctions of epithelial and endothelial cells and is involved in the regulation of junctional integrity and permeability)

Type of Antibody: Monoclonal

Species Raised in: Rabbit

Species reacts against: Reacts with Rabbit, Mouse, Human

Types of Application: Western Blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

References: <pubmed>23001478</pubmed>


Types of Application


--Z3399239 (talk) 15:09, 10 April 2014 (EST)

Lab 6

--Z3399239 (talk) 15:29, 17 April 2014 (EST)

B35 graph.png

Lab 7

--Z3399239 (talk) 16:55, 1 May 2014 (EST)

Lab 8

--Z3399239 (talk) 12:42, 15 May 2014 (EST)

Group 2 Peer-Review

Introduction: Good introduction and outline of mitochondria that shows development of knowledge beyond lecture material. Significant research is evident and well referenced/cited. Good use of imaging though possibly more descriptive diagrams could be used? Consider changing the first sentence from "The mitochondria are..." to just "Mitochondria are..."

Protein Transport Into Mitochondria: Well referenced, relevant material. The diagram was not very well explained in my opinion and could use further development in describing what it is illustrating. The information presented looks good. I was confused by the statement "Such subunits are TIM17, TIM23 and TIM44 which are found in a ratio of roughly 2:2:2" would the ratio be the same as 1:1:1 or are you making a point that they function as dimers?

Pyruvate Into the Mitochondria: Good referencing. Looks a little under-developed but that may be due to lack of current understanding. Diagrams would help greatly here.

What can go wrong with Transport into Mitochondria: Good referencing up until the last two paragraphs. In line 4 should it be "It is made in the cytosol and imported into the mitochondria via the (TOM) complex.". Consider revising "When disrupted and the mitochondrial is damaged and it loses its membrane potential,". This section could also be further developed and the picture associated explained.

Current or Future Research: Needs further development and information as well as diagrams. Some referencing present.

Overall: Some good content and referencing. Knowledge and research shows understanding that goes beyond lecture material. Relatively accessible to peers though more diagrams with descriptions would be helpful. The Glossary was a nice addition and could be further expanded. There are a number of repeats in the reference list, namely "Sébastien Herzig, Etienne Raemy, Sylvie Montessuit, Jean-Luc Veuthey, Nicola Zamboni, Benedikt Westermann, Edmund R S Kunji, Jean-Claude Martinou Identification and functional expression of the mitochondrial pyruvate carrier.". Headings and sub-headings are appropriate and easy to follow.

Group 3 Peer-Review