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Lab 2 Individual Assessment
Confocal Laser Scanning Microscopy for Detection of Schistosoma mansoni Eggs in the Gut of Mice
As the name of the journal entails, confocal laser scanning microscopy (CLSM) is used and compared to other imaging techniques. The aim is to detect Schistosoma mansoni eggs in the gut of mice and conclude whether CLSM is a viable and more effective and efficient method than other imagine techniques.
Schistosoma mansion eggs are direct indicators of schistosomiasis. schistosomiasis infects the urinary tract or intestines. Symptoms may include: abdominal pains, diarrhea, bloody stool, or blood in the urine. For long term sufferers and late diagnosis the effects can be liver damage, kidney failure, infertility, or bladder cancer.
The current and best way to detect schistosomiasis is detecting eggs which possess a characteristic spine from urine, stool, or rectal and bladder biopsy specimens. As sound as the current methods are, urine and stool samples do not always test positively to indicate schistosomiasis due to the viability of eggs. The dissected specimen undergoes various staining methods which can reveal different levels of egg maturity.
The results showed CLSM had a much better detection rate of all different egg maturities and thus, can be used as a more effective method of detecting schistosomiasis.
- <pubmed> 21533168</pubmed>