Difference between revisions of "User:Z3378012"

From CellBiology
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==Lab 2 Individual Assessment==
 
==Lab 2 Individual Assessment==
  
=Test=
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'''Confocal Laser Scanning Microscopy for Detection of Schistosoma mansoni Eggs in the Gut of Mice'''
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[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018799 PLoS One]
 
[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018799 PLoS One]
  
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Background
 
Background
  
The gold standard for diagnosing Schistosoma mansoni infections is the detection of eggs from stool or biopsy specimens. The viability of collected eggs can be tested by the miracidium hatching procedure. Direct detection methods are often limited in patients with light or early infections, whereas serological tests and PCR methods fail to differentiate between an inactive and persistent infection and between schistosomal species. Recently, confocal laser scanning microscopy (CLSM) has been introduced as a diagnostic tool in several fields of medicine. In this study we evaluated CLSM for the detection of viable eggs of S. mansoni directly within the gut of infected mice.
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As the name of the journal entails, confocal laser scanning microscopy (CLSM) is used and compared to other imaging techniques. The aim is to detect Schistosoma mansoni eggs in the gut of mice and conclude whether CLSM is a viable and more effective and efficient method than other imagine techniques.  
 
 
Methodology/Principal Findings
 
  
The confocal laser scanning microscope used in this study is based on the Heidelberg Retina Tomograph II scanning laser system in combination with the Rostock Cornea Module (image modality 1) or a rigid endoscope (image modality 2). Colon sections of five infected mice were examined with image modalities 1 and 2 for schistosomal eggs. Afterwards a biopsy specimen was taken from each colon section and examined by bright-field microscopy. Visualised eggs were counted and classified in terms of viability status.
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Schistosoma mansion eggs are direct indicators of schistosomiasis. schistosomiasis infects the urinary tract or intestines. Symptoms may include: abdominal pains, diarrhea, bloody stool, or blood in the urine. For long term sufferers and late diagnosis the effects can be liver damage, kidney failure, infertility, or bladder cancer.  
  
Conclusions/Significance
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The current and best way to detect schistosomiasis is detecting eggs which possess a characteristic spine from urine, stool, or rectal and bladder biopsy specimens. As sound as the current methods are, urine and stool samples do not always test positively to indicate schistosomiasis due to the viability of eggs. The dissected specimen undergoes various staining methods which can reveal different levels of egg maturity.
  
We were able to show that CLSM visualises eggs directly within the gut and permits discrimination of schistosomal species and determination of egg viability. Thus, CLSM may be a suitable non-invasive tool for the diagnosis of schistosomiasis in humans.
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The results showed CLSM had a much better detection rate of all different egg maturities and thus, can be used as a more effective method of detecting schistosomiasis.
  
  

Revision as of 02:45, 27 March 2014

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Add an external Link.

PubMed

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INTERNAL LINK

this is awesome

This is about prokaryote. [1]

This is also about prokaryotes. [2]

<pubmed limit=2>mitochondria</pubmed>

Images

Ecoli [3]

Journal.pone.0091915.g003.png[4]

Lab 2 Individual Assessment

Confocal Laser Scanning Microscopy for Detection of Schistosoma mansoni Eggs in the Gut of Mice

PLoS One

Schistosoma mansoni Eggs in the Gut of Mice[5]

Background

As the name of the journal entails, confocal laser scanning microscopy (CLSM) is used and compared to other imaging techniques. The aim is to detect Schistosoma mansoni eggs in the gut of mice and conclude whether CLSM is a viable and more effective and efficient method than other imagine techniques.

Schistosoma mansion eggs are direct indicators of schistosomiasis. schistosomiasis infects the urinary tract or intestines. Symptoms may include: abdominal pains, diarrhea, bloody stool, or blood in the urine. For long term sufferers and late diagnosis the effects can be liver damage, kidney failure, infertility, or bladder cancer.

The current and best way to detect schistosomiasis is detecting eggs which possess a characteristic spine from urine, stool, or rectal and bladder biopsy specimens. As sound as the current methods are, urine and stool samples do not always test positively to indicate schistosomiasis due to the viability of eggs. The dissected specimen undergoes various staining methods which can reveal different levels of egg maturity.

The results showed CLSM had a much better detection rate of all different egg maturities and thus, can be used as a more effective method of detecting schistosomiasis.


References

  1. <pubmed>24603758</pubmed>
  2. <pubmed>24601599</pubmed>
  3. <pubmed>24603758</pubmed>
  4. <pubmed>24637574</pubmed>
  5. <pubmed> 21533168</pubmed>