User:Z3374116

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Revision as of 15:40, 21 May 2015 by Z3374116 (talk | contribs) (Peer Review Assessment)

--Z3374116 (talk) 16:44, 12 March 2015 (EST)

Attendance

Lab 1: --Z3374116 (talk) 16:35, 12 March 2015 (EST)

Lab 2: --Z3374116 (talk) 16:40, 19 March 2015 (EST)

Lab 3:

Lab 4: --Z3374116 (talk) 16:19, 2 April 2015 (EST)

Lab 5:

Lab 6: --Z3374116 (talk) 16:01, 23 April 2015 (EST)

Lab 7: --Z3374116 (talk) 16:06, 30 April 2015 (EST)

Lab 8: --Z3374116 (talk) 17:13, 7 May 2015 (EST)

Lab 9: --Z3374116 (talk) 16:48, 14 May 2015 (EST)

Lab 10:

Individual Lab Assessments

Lab Assessment 1

Cyanobacterial cell death.png[1]

Later stage cyanobacterial cell death visualized by the TUNEL assay.

Lab Assessment 2

This Research article explains the achievement of reaching a spatial differentiation which is not limited to light as well as introducing the new and highly technological Super Resolution Florescence Microscopy methods. It addresses the benefits and advances made within the present time and past in regards to optical microscopes leading to the birth of Super resolution fluorescence microscopy techniques. This article focuses mainly on the different branches and types of Super Resolution Microscopy and their different parameters and uses in cell biology observations.

The article addresses the limitations of current optical microscopy, mostly about the diffraction which light undergoes when observing things in high magnification. Super Resolution Florescence Microscopy has managed to overcome these light diffraction barriers providing high resolution imagery as well as being able to observe specimens and structures in a live sample in comparison to other methods.[2]

Lab Assessment 3

Article 1

Research article breaks down the main components of Elastin describing its characteristics. Observations on the alternating hydrophobic and cross-linking characteristics within Elastin are described. Focuses on explaining in depth the basis of self-organizational ability of elastin-based polypeptides and how the information from them can possibly assist in developing self-assembling biomaterials.

<pubmed>11911775</pubmed>

Article 2

<pubmed>25811498</pubmed>

Article 3

This article focuses on investigating the mechanisms behind the elastic fiber assembly via observing the molecular interactions between 'elastin' and 'microfibrillar' components using solid-phase binding assays. Observations found that the major cross-linking region in elastin is formed by association of domains encoded by exons 10.19 and 25 of tropoelastin.

<pubmed>8575256</pubmed>

Article 4

<pubmed>1648323</pubmed>

Article 5

<pubmed>18228265</pubmed>

Lab Assessment 5

Count of Undifferentiated B35 Cells of Different Phenotypes.PNG

Graph representing the phenotypic changes of control B35 cells as well as overexpressed Tm4 (Tropomyosin 4) B35 cells.

Lab Assessment 6

1.Identify an antibody that can been used in your group's extracellular matrix project.

Anti-Elastin Antibody (ab21610)

2. Identify the species deriving the antibody.

Rabbits

3. Identify the working concentration for the antibody.

Application / Dilution

IHC-FoFr 1/50 ~ 1/100

ELISA 1/1000 ~ 1/2000

4. Identify a secondary antibody that could be used with this antibody.

FITC-conjugated secondary antibody

5. Identify a paper that has used this antibody.

<pubmed>PMC3351119</pubmed>

Peer Review Assessment

Group 1

Straight away, the wiki-page is visibly pleasing. The information seems to be organized well under their respective subheadings as well as broken down nicely within their sections to avoid mass clumping of information. It was nice to have an introduction that described simply the function and structure of Proteoglycans, and it was also great to see that you have mentioned which specific topics you will be talking about throughout the page. The history section was used greatly to break down previous, current and future possibilities in research with Proteoglycans as well as a nicely broken down section on structure. I think that the text under the subheading 'structure' can be improved by maybe incorporating some bolding of words (would look simpler and less like a wall of text). Love the table that was used in your project for structure as well as how you broke down the individual components and proceeded to describe them, giving us the reader the most information.

I think maybe you might want to add more information on the synthesis of Proteoglycans, maybe add a video or even a flow diagram, but overall it is well done. I also think that your 'Disease' section is slightly lengthy and too much of a wall of text in comparison to your other sections, the amount of text in this section is overwhelming compared to that in the others (which means you did significant research). Great citation and all your images seem to follow the correct guidelines provided by Mark!

Great job overall! Keep at it!

Group 2

Having a quick initial look through your project, there seems to be a lot of quality and in-depth information under their respective correct subheadings. The structure section was described in a simple yet very descriptive way allowing me to understand the basic outline of what an Integrin consisted of. There are some parts of this section which is still under construction, nevertheless it is still well written. The function section provides some nice base information with a nice video (there is no sound?) however the video seems rather fast and I had to re-watch it a few times, maybe slowing it down if possible would be great but other than that, the images were great and correctly cited. It was great to see how you talked about Integrin and its interaction with other molecules around it and how it went about in their interactions.

The Disease section is very informative, but it is very hard to read the wall of text. Maybe incorporate images related to the diseases in between the text would be better in breaking up the information, but other than that, you did provide clear concise information on the role of each Integrin in the disease so well done!

In terms of improvement, I think more information on History and Timeline can be provided as well as some information on the synthesis and regulation section of your project. The project is coming together nicely! Well done!

Group 4

The information in this project seems to be organised well into simple subheadings which allows ease of access for readers to go to their desired section when reading through the project. The introduction has great descriptive information breaking down the structural aspects of Fibronectin as well as lightly touching on the function as well (might be slightly too much for an introduction though). The history section of the project is nicely put, however there seems to be no historical data post 1980s? And also tabulating the data may be aesthetically pleasing.

The structure section is informative and describes the function, form and interaction of all its individual components, this was nicely done, and the break-down of text made it much easier to read and access information. I think this section could be improved by adding a structural image on the side of the text to allow the readers to understand what these components look like or how they interact with one another. This can be said with the assembly section, maybe make use of a flow diagram with your information to show a simplified process of Fibronectin formation.There is abundant information in the Function and Abnormalities section, but as of now it seems like a massive wall of information (good information), which can be addressed by adding in more images in-text or on the side.

Overall, the information is all there on this page! I think it would be nice to add those pictures (you do need a hand-drawn image to meet assessment criteria) to the relevant sections as well as information on the current research to see where we stand on our understanding of Fibronectins. Other than that, great job guys!

References

Reference

  1. <pubmed>23822984</pubmed>
  2. <pubmed>19489737</pubmed>


Useful Resources

Resources
Pubmed
PLoS
Mark Hills Test Page

Cells Eukaryotes and Prokaryotes

PMID 25513760

<pubmed>25513760</pubmed> <pubmed>25754732</pubmed>