User:Z3374116

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Revision as of 15:06, 21 May 2015 by Z3374116 (talk | contribs)

--Z3374116 (talk) 16:44, 12 March 2015 (EST)

Attendance

Lab 1: --Z3374116 (talk) 16:35, 12 March 2015 (EST)

Lab 2: --Z3374116 (talk) 16:40, 19 March 2015 (EST)

Lab 3:

Lab 4: --Z3374116 (talk) 16:19, 2 April 2015 (EST)

Lab 5:

Lab 6: --Z3374116 (talk) 16:01, 23 April 2015 (EST)

Lab 7: --Z3374116 (talk) 16:06, 30 April 2015 (EST)

Lab 8: --Z3374116 (talk) 17:13, 7 May 2015 (EST)

Lab 9: --Z3374116 (talk) 16:48, 14 May 2015 (EST)

Lab 10:

Individual Lab Assessments

Lab Assessment 1

Cyanobacterial cell death.png[1]

Later stage cyanobacterial cell death visualized by the TUNEL assay.

Lab Assessment 2

This Research article explains the achievement of reaching a spatial differentiation which is not limited to light as well as introducing the new and highly technological Super Resolution Florescence Microscopy methods. It addresses the benefits and advances made within the present time and past in regards to optical microscopes leading to the birth of Super resolution fluorescence microscopy techniques. This article focuses mainly on the different branches and types of Super Resolution Microscopy and their different parameters and uses in cell biology observations.

The article addresses the limitations of current optical microscopy, mostly about the diffraction which light undergoes when observing things in high magnification. Super Resolution Florescence Microscopy has managed to overcome these light diffraction barriers providing high resolution imagery as well as being able to observe specimens and structures in a live sample in comparison to other methods.[2]

Lab Assessment 3

Article 1

Research article breaks down the main components of Elastin describing its characteristics. Observations on the alternating hydrophobic and cross-linking characteristics within Elastin are described. Focuses on explaining in depth the basis of self-organizational ability of elastin-based polypeptides and how the information from them can possibly assist in developing self-assembling biomaterials.

<pubmed>11911775</pubmed>

Article 2

<pubmed>25811498</pubmed>

Article 3

This article focuses on investigating the mechanisms behind the elastic fiber assembly via observing the molecular interactions between 'elastin' and 'microfibrillar' components using solid-phase binding assays. Observations found that the major cross-linking region in elastin is formed by association of domains encoded by exons 10.19 and 25 of tropoelastin.

<pubmed>8575256</pubmed>

Article 4

<pubmed>1648323</pubmed>

Article 5

<pubmed>18228265</pubmed>

Lab Assessment 5

Count of Undifferentiated B35 Cells of Different Phenotypes.PNG

Graph representing the phenotypic changes of control B35 cells as well as overexpressed Tm4 (Tropomyosin 4) B35 cells.

Lab Assessment 6

1.Identify an antibody that can been used in your group's extracellular matrix project.

Anti-Elastin Antibody (ab21610)

2. Identify the species deriving the antibody.

Rabbits

3. Identify the working concentration for the antibody.

Application / Dilution

IHC-FoFr 1/50 ~ 1/100

ELISA 1/1000 ~ 1/2000

4. Identify a secondary antibody that could be used with this antibody.

FITC-conjugated secondary antibody

5. Identify a paper that has used this antibody.

<pubmed>PMC3351119</pubmed>

Peer Review Assessment

Group 1

Straight away, the wiki-page is visibly pleasing. The information seems to be organized well under their respective subheadings as well as broken down nicely within their sections to avoid mass clumping of information. It was nice to have an introduction that described simply the function and structure of Proteoglycans, and it was also great to see that you have mentioned which specific topics you will be talking about throughout the page. The history section was used greatly to break down previous, current and future possibilities in research with Proteoglycans as well as a nicely broken down section on structure. I think that the text under the subheading 'structure' can be improved by maybe incorporating some bolding of words (would look simpler and less like a wall of text). Love the table that was used in your project for structure as well as how you broke down the individual components and proceeded to describe them, giving us the reader the most information.

I think maybe you might want to add more information on the synthesis of Proteoglycans, maybe add a video or even a flow diagram, but overall it is well done. I also think that your 'Disease' section is slightly lengthy and too much of a wall of text in comparison to your other sections, the amount of text in this section is overwhelming compared to that in the others (which means you did significant research). Great citation and all your images seem to follow the correct guidelines provided by Mark!

Great job overall! Keep at it!


References

Reference

  1. <pubmed>23822984</pubmed>
  2. <pubmed>19489737</pubmed>


Useful Resources

Resources
Pubmed
PLoS
Mark Hills Test Page

Cells Eukaryotes and Prokaryotes

PMID 25513760

<pubmed>25513760</pubmed> <pubmed>25754732</pubmed>