Individual Lab Assessments
Lab Assessment 1
Later stage cyanobacterial cell death visualized by the TUNEL assay.
Lab Assessment 2
This Research article explains the achievement of reaching a spatial differentiation which is not limited to light as well as introducing the new and highly technological Super Resolution Florescence Microscopy methods. It addresses the benefits and advances made within the present time and past in regards to optical microscopes leading to the birth of Super resolution fluorescence microscopy techniques. This article focuses mainly on the different branches and types of Super Resolution Microscopy and their different parameters and uses in cell biology observations.
The article addresses the limitations of current optical microscopy, mostly about the diffraction which light undergoes when observing things in high magnification. Super Resolution Florescence Microscopy has managed to overcome these light diffraction barriers providing high resolution imagery as well as being able to observe specimens and structures in a live sample in comparison to other methods.
Lab Assessment 3
Research article breaks down the main components of Elastin describing its characteristics. Observations on the alternating hydrophobic and cross-linking characteristics within Elastin are described. Focuses on explaining in depth the basis of self-organizational ability of elastin-based polypeptides and how the information from them can possibly assist in developing self-assembling biomaterials.
This article focuses on investigating the mechanisms behind the elastic fiber assembly via observing the molecular interactions between 'elastin' and 'microfibrillar' components using solid-phase binding assays. Observations found that the major cross-linking region in elastin is formed by association of domains encoded by exons 10.19 and 25 of tropoelastin.
Lab Assessment 5
Graph representing the phenotypic changes of control B35 cells as well as overexpressed Tm4 (Tropomyosin 4) B35 cells.
Lab Assessment 6
1.Identify an antibody that can been used in your group's extracellular matrix project.
Anti-Elastin Antibody (ab21610)
2. Identify the species deriving the antibody.
3. Identify the working concentration for the antibody.
Application / Dilution
IHC-FoFr 1/50 ~ 1/100
ELISA 1/1000 ~ 1/2000
4. Identify a secondary antibody that could be used with this antibody.
FITC-conjugated secondary antibody
5. Identify a paper that has used this antibody.
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