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====Research Article Using Confocal Microscopy==== | ====Research Article Using Confocal Microscopy==== | ||
− | + | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3556594/| Metamorphosis of mesothelial cells with active horizontal motility in tissue culture] | |
The aim of this study was to investigate and provide evidence to support the migration and morphological change of mesothelial cells during tissue injury response. By using confocal microscopy imaging Nagai and peers, 2013, were able to observe clearly delineated mesothelial cells within ex vivo mouse abdominal wall tissue culture, as well as transformed mesothelial cells (MeT5A) in in-vivo tissue culture and witness their morphological transition from flat cells to cuboidal cells. This transition was able to be observed as confocal microscope analysis allows for the control in the depth of field and eliminates/reduces background matter, so that mesothelial cells positioned on top of each other can be differentiated. In addition, these confocal microscopy techniques also allowed the researchers to observe the horizontal migration of these mesothelial cells across the tissue culture, moving through gaps between surrounding cells. These findings provided evidence in support of the change and migration of mesothelial cells during injury response. <ref><pubmed>23359855</pubmed></ref> | The aim of this study was to investigate and provide evidence to support the migration and morphological change of mesothelial cells during tissue injury response. By using confocal microscopy imaging Nagai and peers, 2013, were able to observe clearly delineated mesothelial cells within ex vivo mouse abdominal wall tissue culture, as well as transformed mesothelial cells (MeT5A) in in-vivo tissue culture and witness their morphological transition from flat cells to cuboidal cells. This transition was able to be observed as confocal microscope analysis allows for the control in the depth of field and eliminates/reduces background matter, so that mesothelial cells positioned on top of each other can be differentiated. In addition, these confocal microscopy techniques also allowed the researchers to observe the horizontal migration of these mesothelial cells across the tissue culture, moving through gaps between surrounding cells. These findings provided evidence in support of the change and migration of mesothelial cells during injury response. <ref><pubmed>23359855</pubmed></ref> |
Revision as of 21:32, 27 March 2013
Contents
Lab Attendance
--Z3331556 (talk) 15:15, 14 March 2013 (EST)
--Z3331556 (talk) 15:38, 21 March 2013 (EST)
Lab 1 Activities
Internal Link
External Link
Inserting Image
Dot Points
- point 1
- point 2
- point 3
Individual Assessments
Lab 1
The Origin of Eukaryotes from Prokaryotes by Time and Genetic Distance
Nick Lane Energetics and genetics across the prokaryote-eukaryote divide. Biol. Direct: 2011, 6();35 PMID:21714941
Copyright ©2011 Lane; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Lab 2
Endosymbiotic origin of eukaryotes by time and genetic distance [1]
Research Article Using Confocal Microscopy
Metamorphosis of mesothelial cells with active horizontal motility in tissue culture
The aim of this study was to investigate and provide evidence to support the migration and morphological change of mesothelial cells during tissue injury response. By using confocal microscopy imaging Nagai and peers, 2013, were able to observe clearly delineated mesothelial cells within ex vivo mouse abdominal wall tissue culture, as well as transformed mesothelial cells (MeT5A) in in-vivo tissue culture and witness their morphological transition from flat cells to cuboidal cells. This transition was able to be observed as confocal microscope analysis allows for the control in the depth of field and eliminates/reduces background matter, so that mesothelial cells positioned on top of each other can be differentiated. In addition, these confocal microscopy techniques also allowed the researchers to observe the horizontal migration of these mesothelial cells across the tissue culture, moving through gaps between surrounding cells. These findings provided evidence in support of the change and migration of mesothelial cells during injury response. [2]