Tubular structures found in tissues and cultures. Naturally occurring and stress induced tubular structures from mammalian cells, a survival mechanism Yonnie Wu, Richard C Laughlin, David C Henry, Darryl E Krueger, JoAn S Hudson, Cheng-Yi Kuan, Jian He, Jason Reppert, Jeffrey P Tomkins BMC Cell Biology 2007, 8:36 (16 August 2007) © 2007 Wu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The aim of the experiment in this research article was to demonstrate the fundamental role of the membrane-bound metalloproteinase MT1-MMP catalyst in preparing collagen fibrils for phagocytic degradation of collagen in adult tissues. Confocal laser microscopy has been utilized in this experiment to show that human fibroblasts do in fact initiate degradation of collagen through the collagenase activity of the membrane-bound metalloproteinase MT1-MMP. And examination of the collagen degradation under a confocal microscope revealed that cell surface MT1-MMP was in fact associated with degrading collagen fibrils.