Structural Model of Bacterial and Eukaryotic Microtubules 
Reference: Pilhofer M, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ (2011) Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton. PLoS Biol 9(12): e1001213. doi:10.1371/journal.pbio.1001213
Copyright: © 2011 Pilhofer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Identify a recent research article (not review) that uses either confocal microscopy or super-resolution microscopy as one of the study's techniques. Explain briefly (1 paragraph) how the microscopy technique specifically contributed to the article's findings.
This study examined the chemotherapeutic drug doxorubicin (DOX) with the novel sixth-generation cationic poly-l-lysine dendrimer (DM) and determined enhanced therapeutic efficacy of DOX when complexed to DM in tumour-bearing mice. Confocal microscopy allowed evaluation of the depth of distribution of both drugs (DOX and DOX-DM) into the Multicellular Tumour Spheroids.
To evaluate the depth of distribution of DOX or DOX–DM into the MTS, the optical probe technique described previously in ref 16 was used. In brief, radial recordings of total fluorescence intensity in a selected region of interest (ROI) were performed in MTS starting from the periphery (spheroid rim) toward the center (equatorial section). The pinhole settings of the confocal microscope were set to produce 4 μm thick sections and a whole z-series of 23 ROIs, allowing scanning until approximately 80–90 μm deep into the spheroid. For each spheroid image, the obtained total intensity was subtracted from background fluorescence obtained from untreated MTS, then corrected for the exponential light attenuation due to scattering and absorption within the spheroid. The linear attenuation coefficient was determined as described previously in ref 50 (see Supporting Information) and found to be 0.003165/μm. The total fluorescence intensity, after background subtraction and correction for scattering, was calculated in each ROI and plotted as a function of the distance from the spheroid rim. DM or DOX fluorescence was expressed in green.
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Referencing: 1. find the number, remove colon.
2. Put pubmed code at front and end.
3. Put ref code at front and end