Difference between revisions of "User:Z3324681"
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Structural Model of Bacterial and Eukaryotic Microtubules 
Reference: Pilhofer M, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ (2011) Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton. PLoS Biol 9(12): e1001213. doi:10.1371/journal.pbio.1001213
Copyright: © 2011 Pilhofer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Identify a recent research article (not review) that uses either confocal microscopy or super-resolution microscopy as one of the study's techniques. Explain briefly (1 paragraph) how the microscopy technique specifically contributed to the article's findings.
This study examined the chemotherapeutic drug doxorubicin (DOX) with the novel sixth-generation cationic poly-l-lysine dendrimer (DM) and determined enhanced therapeutic efficacy of DOX when complexed to DM in tumour-bearing mice. Confocal microscopy allowed evaluation of the depth of distribution of both drugs (DOX and DOX-DM) into the Multicellular Tumour Spheroids (MTS)bu the following method. For each image, intensity from background fluorescence was obtained from untreated MTS and subtracted from total intensity. The total fluoresence intensity was then calculated in each region of interest and plotted as a function of the distance from the spheroid rim. 
--Mark Hill (talk) 11:20, 11 April 2013 (EST)This paper does employ confocal analysis of tumor growth in vivo. You could have explained why this technique has an advantage/application over other methods. FYI - Live animal fluorescence optical imaging was used to monitor DOX retention in vivo of the fluorescent DOX using the IVIS Lumia II imaging system.
1. Select 4 reference papers related to your selected topic sub-section. Read these papers and write a brief description of their findings and relevance to the selected topic sub-section. The reference along with your description should then be pasted on both your group discussion page and your own personal page. 2. Select an image related to your selected topic sub-section (this can be from one of the 4 above or from elsewhere). The image should be uploaded (with all the required information: description, reference, copyright and student template) and pasted onto the project page sub-section and onto your own personal page.
Identify an antibody against an adhesion junction protein that is commercially available. Add a link to the original data sheet page and identify the type of adhesion junction. Include the following information: type of antibody (polyclonal, monoclonal), species raised in, species reacts against, types of application uses, and if available any reference using that antibody.
Tight Junction Protein 1 Antibody
Type of adhesion junction: human zona occludens 1, specifically ZO-1 alpha-minus found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Type of antibody: Polyclonal
Species raised in: Guinea Pig
Species reactivity: Human, Mouse, Rat, and Canine
Types of application uses: Immunohistology, immunofluorescence and Western blotting
References that uses this antibody:
Pictures: Resizing and repositioning
Pictures: Thumbnail and Renaming
Referencing: 1. find the number, remove colon.
2. Put pubmed code at front and end.
3. Put ref code at front and end