Difference between revisions of "User:Z3324681"

From CellBiology
(Individual Assessments)
(Lab 2)
Line 16: Line 16:
 
===Lab 2===
 
===Lab 2===
 
Identify a recent research article (not review) that uses either confocal microscopy or super-resolution microscopy as one of the study's techniques. Explain briefly (1 paragraph) how the microscopy technique specifically contributed to the article's findings.
 
Identify a recent research article (not review) that uses either confocal microscopy or super-resolution microscopy as one of the study's techniques. Explain briefly (1 paragraph) how the microscopy technique specifically contributed to the article's findings.
 +
 +
 +
This study examined the chemotherapeutic drug doxorubicin (DOX) with the novel sixth-generation cationic poly-l-lysine dendrimer (DM) and determined enhanced therapeutic efficacy of DOX when complexed to DM in tumour-bearing mice. Confocal microscopy allowed evaluation of the depth of distribution of both drugs (DOX and DOX-DM) into the Multicellular Tumour Spheroids.
 +
 +
 +
To evaluate the depth of distribution of DOX or DOX–DM into the MTS, the optical probe technique described previously in ref 16 was used. In brief, radial recordings of total fluorescence intensity in a selected region of interest (ROI) were performed in MTS starting from the periphery (spheroid rim) toward the center (equatorial section). The pinhole settings of the confocal microscope were set to produce 4 μm thick sections and a whole z-series of 23 ROIs, allowing scanning until approximately 80–90 μm deep into the spheroid. For each spheroid image, the obtained total intensity was subtracted from background fluorescence obtained from untreated MTS, then corrected for the exponential light attenuation due to scattering and absorption within the spheroid. The linear attenuation coefficient was determined as described previously in ref 50 (see Supporting Information) and found to be 0.003165/μm. The total fluorescence intensity, after background subtraction and correction for scattering, was calculated in each ROI and plotted as a function of the distance from the spheroid rim. DM or DOX fluorescence was expressed in green.
  
 
== References ==
 
== References ==

Revision as of 14:00, 28 March 2013

Lab Attendance

--Z3324681 (talk) 15:54, 14 March 2013 (EST)

--Z3324681 (talk) 15:50, 21 March 2013 (EST)

Individual Assessments

Lab 1

Bacterial and Eukaryotic Microtubule Structure.png

Structural Model of Bacterial and Eukaryotic Microtubules [1]

Reference: Pilhofer M, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ (2011) Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton. PLoS Biol 9(12): e1001213. doi:10.1371/journal.pbio.1001213

Copyright: © 2011 Pilhofer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Lab 2

Identify a recent research article (not review) that uses either confocal microscopy or super-resolution microscopy as one of the study's techniques. Explain briefly (1 paragraph) how the microscopy technique specifically contributed to the article's findings.


This study examined the chemotherapeutic drug doxorubicin (DOX) with the novel sixth-generation cationic poly-l-lysine dendrimer (DM) and determined enhanced therapeutic efficacy of DOX when complexed to DM in tumour-bearing mice. Confocal microscopy allowed evaluation of the depth of distribution of both drugs (DOX and DOX-DM) into the Multicellular Tumour Spheroids.


To evaluate the depth of distribution of DOX or DOX–DM into the MTS, the optical probe technique described previously in ref 16 was used. In brief, radial recordings of total fluorescence intensity in a selected region of interest (ROI) were performed in MTS starting from the periphery (spheroid rim) toward the center (equatorial section). The pinhole settings of the confocal microscope were set to produce 4 μm thick sections and a whole z-series of 23 ROIs, allowing scanning until approximately 80–90 μm deep into the spheroid. For each spheroid image, the obtained total intensity was subtracted from background fluorescence obtained from untreated MTS, then corrected for the exponential light attenuation due to scattering and absorption within the spheroid. The linear attenuation coefficient was determined as described previously in ref 50 (see Supporting Information) and found to be 0.003165/μm. The total fluorescence intensity, after background subtraction and correction for scattering, was calculated in each ROI and plotted as a function of the distance from the spheroid rim. DM or DOX fluorescence was expressed in green.

References

  1. <pubmed>22162949</pubmed>


Formatting Experiments

Linking things within the page and renaming said links: Lecture 1 is the same link as Cell_Biology_Introduction

Linking things outside the page and renaming said links: Google it! is the same link as [1]

Pictures: Resizing and repositioning

Red White Blood cells 01.jpg

Pictures: Thumbnail and Renaming

OMGCELLS

Dot points:

  • wow
  • bang
  • pop

Referencing: 1. find the number, remove colon.

PMID 23393914


2. Put pubmed code at front and end.

(pubmed)23393914(/pubmed)


3. Put ref code at front and end

(ref)(pubmed)23393914(/pubmed)(/ref)