From CellBiology

Attendance List

--Seow Liew 10:46, 10 March 2011 (EST)

--Seow Liew 09:02, 17 March 2011 (EST)

--Seow Liew 09:16, 24 March 2011 (EST)

--Seow Liew 09:33, 31 March 2011 (EST)

--Seow Liew 09:09, 7 April 2011 (EST)

--Seow Liew 10:07, 14 April 2011 (EST)

--Seow Liew 09:02, 21 April 2011 (EST)

--Seow Liew 09:40, 12 May 2011 (EST)

Individual Assessment

Lab 1

1. What are the key cell biology journals?

Trends in Cell Biology, Cell ,Journal of Cell Science, BMC Cell Biology and Nature Journals.

2. Which journals allow reuse of their published content?

Public Library of Science, The Journal of Cell Biology and BMC Cell Biology.

Lab 2

1. Which chromosomes contribute to the nucleolus?

Ribosomal ribonucleic acid (rRNA)

--Mark Hill 07:56, 24 March 2011 (EST) This is not the correct answer. The genes for rRNA are located at the nucleolus, but they are located on different chromosomes. I was after the identity of these chromosomes.

Chromosomes 13, 14, 15, 21 and 22 which contain rRNA genes contribute to the nucleolus and its functions in humans.

2. Identify and add a link to your page of a recent cell biology article using confocal microscopy.

Here is some bold text

Here is some italic text

teaching my favourite students basic stuff

J of cell biology Homepage


Lab 3

This is about frog nucleoli shape changes.[1][2]

1. Find the SDS information for chloroform and identify the hazards associated with this chemical.

Chloroform SDS info


Irritates respiratory tract and causes central nervous system effects, including headache, drowsiness, dizziness.Prolonged exposure may lead to death due to irregular heart beat and kidney and liver disorders.

Ingestion: Causes severe burning in mouth and throat, pain in the chest and vomiting. Skin Contact:

Causes skin irritation resulting in redness and pain.May be absorbed through skin.

Eye Contact:

Vapors causes pain and irritation to eyes.Splashes may cause severe irritation and possible eye damage.

Chronic Exposure:

Prolonged or repeated exposure to vapors may cause damage to the nervous system, the heart and the liver and kidneys.May cause chronic irritation of skin with cracking and drying, and corresponding dermatitis.

Aggravation of Pre-existing Conditions:

Persons with pre-existing skin disorders or eye problems, or impaired liver, kidney or respiratory function may be more susceptible to the effects of the substance.

2. You will need to upload an image and add it to your page, with the reference and copyright information with the image.


FCS follicles: theca cells.[3]

Reference list

  1. <pubmed>21368180</pubmed>
  2. <pubmed>21368160</pubmed>
  3. <pubmed>21232101</pubmed>

Lab 4

1.Identify a commercial supplier of an antibody that relates to your group project topic.

LifeSpan BioSciences,that produces Rabbit Anti-Human TJP2 Polyclonal Antibody which is used to against

synthetic peptide corresponding to a region of Human TJP2.[ /AntibodyDetail.aspx?AntibodyID=81165 info]

2.In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria?

Lab 5

Lab 6

Group 1


Phenotype A: "Fan" phenotype

Phenotype B: "Broken fan" phenotype

Phenotype C: "Stumped" phenotype

Phenotype D: "Prolonged" phenotype

Phenotype E: "Stringed" phenotype

Phenotype F: "Pygnotic" phenotype

1. What are the changes in phenotypes that you observe between group A and group B in your graph?

The cells in group B(control) demonstrated phenotypes B ("broken fan") and C ("stumped") as the most seen phenotypes with cells with phenotype B , 22.5% and cells with phenotype C, 25.0% . The over Tm4 (Tropomyosin 4) expressed cells in group A(Tm4 over-expressed cells) demonstrated phenotype D ("prolonged") as the most seen phenotype with total percentage 32.5%,whereas phenotype E ("stringed") as the second most seen phenotype with total percentage 20%.In short,the two most seen phenotypes in group B were phenotypes B and C, with total percentage altogether, 47.5% whereas the two most seen phenotypes in group A were phenotypes D and E with total percentage altogether,52.5%.The most seen cell phenotype shifted from fan like(group B) to elongated (group A) when Tm4 was over expressed in cells.In conclusion,we could say that Tm4 facilitated the growth events of cell elongation to interact with one another,at the same time inhibited phenotypes B and C.

2. What are the changes in phenotypes that you observe between group A and group B in second part of the lab?

Group A

-the diameter of cytoplasm was relatively less as compared to cells in group B.

-elongated processes extensively branched off the cells.

-processes and cytoplasm appeared yellowish

-the cell colony was orientated in network or web like.

Group B

-the diameter of cytoplasm was relatively large as compared to cells in group A.

-relatively short processes branched off the cells.

-processes and cytoplasm appeared reddish.

-the cell colony was orientated in groups.

In conclusion, Tm4 very much involved in giving branching phenotype to the cells to interact with one another by rearranging the cellular cytoskeletons in accordance to specify ability of Tm4.In addition,ass the events of branching going, the diameter of cytoplasm shrunk and decreased.Notably,cytoplasm of cells of group A appeared more yellowish that cells in group B, this might due to tropomyosin 4 was over expressing in the cells.Thus , this group of cells appeared to be brighter.

3. How does Tm4(tropomyosin 4) mediate these changes?

The tropomyosin 4 proteins involve in Tm 4 expression within the cell.Over expression of the gene will flood the cytoplasm with tropomyosin 4, which will then loosen the filamentous structures within the cytoplasm. The filamentous structures no longer be able to maintain the cell shape , consequently, processes branch off in accordance to the ability of Tm4 on the cell.