Structural features comparison of the proteomes in the three superkingdoms
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In this study the researchers used Super-Resolution Microscopy in order to attempt to understand the gag proteins and envelope recruitment and how exactly the HIV-1 virus is incorporated into the cell (which was previously not known). They were able to see the GAG assembly sites along with teh HIV-1 envelope proteins in the envelope expressing cells. It was discovered that the envelope accumulation outside of and surrounding the GAG assemblies (rather than directly being in contact with it) was able to assist the spread of the virus in vivo.
--Mark Hill (talk) 10:39, 9 April 2013 (EST) This is a reasonable summary of the article on Super-Resolution Microscopy. You may have considered explaining further why this technique was better than existing techniques, e.g. resolution, localisation. You have used as explained in class the Pubmed referencing format correctly.
Antibody against an adhesion junction protein - E-Cadherin
Data sheet: [E-Cadherin]
Type of antibody: Monoclonal
Species raised in: Mouse
Species reacts against: human breast carcinoma cell line T471
Types of application uses: detection by Western blotting, Immunoprecipitation, Immunofluorescence
References using E-cadherin antibody: <pubmed>PMC2907333</pubmed>
Do you see any change in the phenotypes between group A and group B?
Group A (Tm4 over-expressing cells) had a greater percentage of stumped phenotypes and a significantly greater amount of pronged phenotypes compared to group B (control group). Contrastingly, group B had a significantly greater percentage of broken fan phenotypes and a few pygnotic phenotypes were noted while group A had none. Group A and B appeared to have a similar percentage of Fan phenotypes. Notably, group A was seen to have increased branching and processing and brighter fluorescence when compared to group B.
If you see a difference, speculate about a potential molecular mechanism that has lead to this change, if you don't see a change, speculate why that could be.