Difference between revisions of "User:Z3292017"

From CellBiology
(Individual Assessments)
(Individual Assessments)
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'''Group 3'''
 
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I like how you explain in your introduction what you will be covering in the page. It sets it out really well. You have clearly Donna lot of research on the Golgi apparatus and have referenced it well.  I noticed that reference 38 doesn't actually have a reference connected to it? The function section is really informative, the only suggestion I would have here is to add an image or two so that it's easier to read. I think you've done a really good job on the history section and it's well laid out.  In the. Models for division section, if possible I would add some images there to show the differences.  Apart form that the rest of the page is well written and researched with a good number of images to break up the text!  Overall, I think you've done a great job on this page!
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'''Group 4'''
  
  

Revision as of 15:37, 23 May 2013

Lab Attendance

Lab 1 - Z3292017 (talk) 15:07, 14 March 2013 (EST)

Lab 2 - Z3292017 (talk) 15:03, 21 March 2013 (EST)

Lab 3 - Z3292017 (talk) 15:11, 28 March 2013 (EST)

Lab 4 - Z3292017 (talk) 16:12, 11 April 2013 (EST)

Lab 5 - Z3292017 (talk) 15:25, 18 April 2013 (EST)

Lab 6 - Z3292017 (talk) 15:22, 2 May 2013 (EST)

Lab 7 - Z3292017 (talk) 15:29, 9 May 2013 (EST)

Lab 8 - Z3292017 (talk) 15:35, 16 May 2013 (EST)

Individual Assessments

Lab 1


Difference between Prokaryote and Eukaryote Structural Features.jpg

Structural features comparison of the proteomes in the three superkingdoms

Reference

<pubmed>PMC3320622</pubmed>

Copyright

Copyright Pancsa and Tompa. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

--Mark Hill (talk) 10:32, 9 April 2013 (EST) Relevant article on comparison of prokayotes and eukaryotes.


Lab 2

<pubmed>PMC3585150</pubmed>

In this study the researchers used Super-Resolution Microscopy in order to attempt to understand the gag proteins and envelope recruitment and how exactly the HIV-1 virus is incorporated into the cell (which was previously not known). They were able to see the GAG assembly sites along with teh HIV-1 envelope proteins in the envelope expressing cells. It was discovered that the envelope accumulation outside of and surrounding the GAG assemblies (rather than directly being in contact with it) was able to assist the spread of the virus in vivo.


--Mark Hill (talk) 10:39, 9 April 2013 (EST) This is a reasonable summary of the article on Super-Resolution Microscopy. You may have considered explaining further why this technique was better than existing techniques, e.g. resolution, localisation. You have used as explained in class the Pubmed referencing format correctly.


Lab 3


Lab 4


Antibody against an adhesion junction protein - E-Cadherin

Data sheet: [E-Cadherin]

Type of antibody: Monoclonal

Species raised in: Mouse

Species reacts against: human breast carcinoma cell line T471

Types of application uses: detection by Western blotting, Immunoprecipitation, Immunofluorescence

References using E-cadherin antibody: <pubmed>PMC2907333</pubmed>


Lab 6

Tm4 cell graph.png

Questions

Do you see any change in the phenotypes between group A and group B?


Group A (Tm4 over-expressing cells) had a greater percentage of stumped phenotypes and a significantly greater amount of pronged phenotypes compared to group B (control group). Group A also appeared to have a significantly higher number of neurites than group B. Contrastingly, group B had a significantly greater percentage of broken fan phenotypes and a few pygnotic phenotypes were noted while group A had none. Group A and B appeared to have a similar percentage of Fan phenotypes. Notably, group A was seen to have increased branching and processing and brighter fluorescence when compared to group B.


If you see a difference, speculate about a potential molecular mechanism that has lead to this change, if you don't see a change, speculate why that could be.


Tropomyosin is associated with the Actin filament function regulation which stabilizes and increases the strength of the filaments. The Tm4 isoforms are found predominantly in the growth cones of neurons and subsequently when there is an over-expression of Tm4 in the growth cones then in turn it could increase the growth of the neurites [1]. As noted in question one, there was an increased number of neurites in group A (Tm4) than group B (control) which would therefore indicate that the over expression of Tm4 not only increases the growth of the neurites in length but also increases the number of neurites detected. As tropomyosin stabilizes and increases the strength of the actin filaments, this in turn could therefore enable the actin chains to become longer and hence potentially increase the length of the neurites [2]. Due to the increase in length of the neurites this could correlate to the increased number of pronged phenotypes and a substantially decreased number of broken fan phenotypes.


Lab 8

Group 1

It is obvious that you have done a fair amount of research into the regulation of cell division. However something which stood out to me from the introduction was the lack of references. You should look at referencing every new point you make on the page, and the first two paragraphs of the introduction have no references listed. The references you have used appear to be referenced correctly and you have used journal articles as opposed to website. Your history section looks good, but I'm assuming it is still unfinished. I liked the brief description that you made for each date which makes it easy and interesting to read. The images you have used appear to be referenced correctly with the copyright information which is good. The only suggestion I would have is to have a title underneath the image on the actual page so that we know what we are clicking I.e. fig 1 shows this... Another suggestion is to add some more images as I find this breaks up the text and also helps us understand what you're writing about. In regards to your current research section, I'm not entirely sure why you were listing points? Was that what one research article was about or is that a list of articles? This should probably be made a bit clearer and again referenced. Another thing i noticed is that you dnt have a glossary which is a good way to explain uncommon words. I think that its well written and easy to understand but even in the introduction There are several words such as cell division and mitogens which would be good to put in the glossary section. Overall Ithink you've done a good job and if you fix up the images, glossary and the referencing then it should be a great page to read.

Group 2

I think your introduction is very sound and easy to understand however it isn't referenced at all? The images you have included are a good way to show us. What you are writing about. Your history section might be a bit too brief, for example, what physical manipulations did Ray use? And the colours seem to be a bit intermittent, however what you have written is interesting and easy to understand. The mechanisms section is good and easy to understand. As there is a lot of content I would probably suggest adding some more pictures to break up the page and also make it easier for us to read. The two explanations of cytokinesis in animals and plants is good and simple t understand. The Cytokiensis failure section is done quite well with some good images as is the current research. Your glossary is quite in depth which is really good. The only other suggestion I would have is adding some more references to your page. Overall I think you've dne a good job!

Group 3

I like how you explain in your introduction what you will be covering in the page. It sets it out really well. You have clearly Donna lot of research on the Golgi apparatus and have referenced it well. I noticed that reference 38 doesn't actually have a reference connected to it? The function section is really informative, the only suggestion I would have here is to add an image or two so that it's easier to read. I think you've done a really good job on the history section and it's well laid out. In the. Models for division section, if possible I would add some images there to show the differences. Apart form that the rest of the page is well written and researched with a good number of images to break up the text! Overall, I think you've done a great job on this page!

Group 4


References

  1. <pubmed>PMC1165423</pubmed>
  2. <pubmed>9143561</pubmed>