From CellBiology
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Lab attendance

--Nathan Weller 10:42, 10 March 2011 (EST)

--Nathan Weller 09:11, 17 March 2011 (EST)

--Nathan Weller 09:54, 24 March 2011 (EST)

--Nathan Weller 09:23, 31 March 2011 (EST)

--Nathan Weller 09:09, 7 April 2011 (EST)

--Nathan Weller 10:45, 14 April 2011 (EST)

--Nathan Weller 09:22, 21 April 2011 (EST) --Nathan Weller 09:23, 5 May 2011 (EST)

Lab 1 questions=

1) What are the key cell biology journals? 
  • Journal of cell biology
  • Nature of cell biology
  • Trends in cell biology
  • Cell
  • Public library of science
2) Which journals allow you to reuse their published content
  • PLOS
  • Journal of cell biology
  • BMC cell biology

Work area

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Intro lab

Cell bio

Lab 2 questions

--Mark Hill 08:00, 24 March 2011 (EST) Hi. WHere are your lab 2 answers?

  1. Which chromosomes contribute to the nucleolus?

Chromosomes 13 14 15 21 22 contribute to the nucleolus which is the site for ribosomal RNA transcription.

  1. Identify and add a link to your page of a recent cell biology article using confocal microscopy from the Pubmed database.
  • "Confocal imaging from deep brain tissue" [1] |

Work area

Lab 3 questions

1) "Safety data sheet for chloroform"

Has been noted as cancer causing in laboratory animals and is also a carcinogen in humans. Exposure through inhaling or ingestion is toxic and can be fatal. Dermatitis can be caused by exposure to the skin.

2) Pic 2.jpg

Lab 4 Questions

1. Identify a commercial supplier of an antibody that relates to your group project topic.

Abcam is a supplier of antibodies relating to synaptic junctions. They produce Piccolo antibody-A synaptic marker. [2]

2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria?

The gene that encode Cytochrome C is located in the nucleus of the cell. This protein is a part of the electron transport chain location in the inner membrane of the mitochondria. This protein is soluble in water and is contained by the outer membrane of the mitochondria.

Lab 6 questions

[Cell bio graph.jpeg]

1 a) What are the changes in phenotypes that you observe between group A and B?


Group A contains Tm4 and Group B is the control.

-Phenotype A shows no Tm4. -Phenotype B and C are similar in ratio with less Tm4 compared to the control. -Phenotype D and F shows a significant increase in Tm4 compared to the control. -Phenotype E shows a slightly higher amount of Tm4 than the control.


Group A there is a generally pink appearance with yellow edges and blue nuclei. Prolonged phenotype and cells are likely to be close together.

Group B they are red with purple nuclei and fanned phenotype. The cells are bound together.

b) How does Tm 4 mediate these changes Tm4 is an actin-binding protein and is a stabilizer in the cytoskeleton of non muscle cells. It has mediated the differences in the two groups as it has caused a prolonged phenotype in the cells in A whereas there is a more fan like appearance in B. This is because the Tm4 has stabilized the actin filaments in the cells in Group A hence the prolonged phenotype.

Random Work

To understand what a synaptic junction is and what its purpose is within the human body, a brief understanding of the Nervous System must be acquired. The nervous system controls the actions of the body while also transmitting information to different parts of it, and consists of the central and peripheral nervous systems. The brain and spinal cord make up the central nervous system (CNS) while nerves and ganglia which span out from the spinal cord to limbs and organs make up the Peripheral Nervous System (PNS). Information including sensory stimuli, motor control and involuntary activity are all communicated throughout the body by the nervous system. Neurons are a specialised cell which form the network of nerves, spinal cord and brain and will be explained in detail how they play a key role in electrical signalling later on. Electrical signals that carry information throughout the body are transmitted through the nervous system by the communication of these neurons, the Synaptic Junction is where neurons transmit this electrical current to one another. Without Synaptic junctions, information and signals cannot be transmitted to areas of the body, its structure, mechanics dysfunctions and current related topics will be explored in detail.