Difference between revisions of "User:Z3289738"

From CellBiology
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* A researcher has a project for a student on a newly discovered protein called by the discoverer "Musashi".
 
* A researcher has a project for a student on a newly discovered protein called by the discoverer "Musashi".
 +
 
Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Musashi contributes to the maintenance of neural stem cells. While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells.  
 
Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Musashi contributes to the maintenance of neural stem cells. While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells.  
 +
  
 
Musashi antibody #2541
 
Musashi antibody #2541
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* Recommended Antibody Dilution: Wesetern blotting 1:1000; Immunofluorescence (IF-F) 1:25.
 
* Recommended Antibody Dilution: Wesetern blotting 1:1000; Immunofluorescence (IF-F) 1:25.
 
* Specificity/Sensitivity: Musashi Antibody detects endogenous levels of total Musashi 1 and 2 protein.
 
* Specificity/Sensitivity: Musashi Antibody detects endogenous levels of total Musashi 1 and 2 protein.
 +
  
 
Anti-rabit secondary antibody:  
 
Anti-rabit secondary antibody:  

Revision as of 14:30, 29 March 2012

Lab Attendance

  1. Lab 1: --Z3289738 15:29, 8 March 2012 (EST)
  2. Lab 2: --Z3289738 14:10, 15 March 2012 (EST)
  3. Lab 3: --Z3289738 14:04, 22 March 2012 (EST)
  4. Lab 4: --Z3289738 14:51, 29 March 2012 (EST)

Lab 1 - Introduction

  • 'External link' has one [ ]
  • 'Internal link' has two [[ ]]

External Link

http://www.qbi.uq.edu.au/

or

QBI - Queensland Brain Institute

Internal Link

Lab 1

Lab 2 - Microscopy

Uploading a picture onto the wiki:

  1. Save picture to desktop
  2. 'Toolbox' - 'Upload file'
  3. Copy and paste the title into the wiki, put [[__]]

eg. Chlamydomonas cells with flagella, plasma membrane, nucleus, and chloroplast nucleoids highlighted in neon..png

Figure 1: Chlamydomonas cells with flagella, plasma membrane, nucleus, and chloroplast nucleoids highlighted in neon

Robinson R (2007) Centrioles Position the Nucleus and One Another. PLoS Biol 5(6): e161. doi:10.1371/journal.pbio.005016



Homework Lab 2

Identify a reference article that uses the "superresolution" microscopy technique.

Brown TA, Tkachuk AN, Shtengel G, Kopek BG, Bogenhagen DF, Hess HF, Clayton DA. (2011). Superresolution fluorescence imaging of mitochondrial nucleoids reveals their spatial range, limits, and membrane interaction. Molecular Cell Biology. PMID: 22006021


What did the paper show that normal microscopy could not show?

Superresolution fluorescence microscopy exceeded previous imaging techniques by allowing scientists to see within the small and highly compartmentalised mitochondria. Using PAML and iPALM techniques, scientists were able to visualize core dimensions and relative locations of mitochondrial nucleoids, discovering that they are much larger than previously anticipated.

Lab 3 - Fixation

* Tissue preservation for slides

Homework Lab 3

Locate a current SDS for one of the fixatives described in today's lab. Identify the properties and hazards associated with that chemical.

Fixative: Chloroform

The SDS for Chloroform can be found at the following website: http://www.jmloveridge.com/cosh/Chloroform%20Spirit.pdf


Properties and hazards associated with Chloroform

Properties:

  • Colourless mobile liquid
  • Chloroform odour
  • Volatile
  • Miscible with water and ethanol

Hazards:

  • Highly flammable
  • Harmful if swallowed
  • Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed.
  • Limited evidence of a carcinogenic effect.
  • Carcinogen Category 3


Identify 4 papers required for your group work project. Cite on the Group Project discussion page and also on your own Individual page. Add one sentence for each as too why they are relevant to your group topic.

Paper 1

Taketo, M Mark. "Shutting down Wnt signal-activated cancer." Nature Genetics 36. (2004): 320-22. DOI: 10.1038/ng0404-320

In this article, New evidence suggests that Wnt signaling can be suppressed or further activated by upstream signals, even though the pathway seems to be constitutively activated by downstream mutations in cancer cells.

Paper 2

Macdonald, Bryan. "Wnt/β-catenin Signaling: Components, Mechanisms, and Diseases." Developmental Cell 17.1 (2009): 9-26. PMID: 19619488

This article highlights some key aspects of Wnt/β-catenin signaling in human diseases including congenital malformations, cancer, and osteoporosis, and discuss potential therapeutic implications

Paper 3

Luu, Hue. "Wnt/β-catenin Signaling Pathway as Novel Cancer Drug Targets." Current Cancer Drug Targets 4. (2004): 653-71. Doi: 1568-0096/04

This review discusses some of the strategies that are being used or can be explored to target key components of the Wnt/β-catenin signaling pathway in rational cancer drug discover.

Paper 4

Giles, R., Johan, H., Clevers, H. “Cought up in a Wnt storm: Wnt signalling in cancer”. Biochimica et Biophysica Acta, (2003). 1653 1-24. http://dx.doi.org/10.1016/S0304-419X(03)00005-2

This review considers the spectra of tumors arising from active Wnt signaling and attempts to place perspective on recent data that begin to elucidate the mechanisms prompting uncontrolled cell growth following induction of Wnt signaling.

Lab 4

Class Activity

Introduction

  • The following is a student exercise to be carried out during 2012 Lab 4.
  • Students will add content to the sub-sections below.
  • --Mark Hill 13:48, 29 March 2012 (EST) All additions should begin with your signature.
  • Followed by a brief answer
  • And/or link to an appropriate resource.
    • Full ref in text - <pubmed>XXXXX</pubmed>
    • reference as a superscript citation.<ref><pubmed>XXXXX</pubmed></ref>

1. Begin

  • A researcher has a project for a student on a newly discovered protein called by the discoverer "Musashi".

Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Musashi contributes to the maintenance of neural stem cells. While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells.


Musashi antibody #2541

  • Source: rabbit
  • Applications: Western Blot, Immunoflourescence (IF-F) Endogenous
  • Species cross reactivity: human and mouse (determined by western blot)
  • Molecular weight: 30kDa
  • Anti-rabbit secondary antibodies must be used to detect this antibody
  • Recommended Antibody Dilution: Wesetern blotting 1:1000; Immunofluorescence (IF-F) 1:25.
  • Specificity/Sensitivity: Musashi Antibody detects endogenous levels of total Musashi 1 and 2 protein.


Anti-rabit secondary antibody:

  • Alexa Fluor 488 goat anti—rabbit IgG.
  • Supplied by Life Technology.
  • Size 0.5mL for $440.
  • It is a green flourescence.
  • Formulated at 2 mg/ml.
  • Specificity/Sensitivity: F(ab’)2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.


Sorry I'm very slow at researching!

References