User:Z3254753

From CellBiology

Individual page for z3254753

Lab Attendance

--z3254753 10:37, 10 March 2011 (EST)

--z3254753 09:18, 17 March 2011 (EST)

--z3254753 09:09, 24 March 2011 (EST)

--z3254753 09:26, 31 March 2011 (EST)

--z3254753 09:25, 7 April 2011 (EST)

--z3254753 09:12, 14 April 2011 (EST)

--z3254753 09:29, 21 April 2011 (EST)

--z3254753 09:21, 5 May 2011 (EST)

--z3254753 09:47, 12 May 2011 (EST)

Individual Assessments

Lab 1

1. What are the key cell biology journals?

The key cell biology journals are;

1. JCB (journal of Cell Biology)

2. Cell, Nature Cell Biology

3. Journal of Cell Science

4. Molecular Biology of the Cell

5. PNAS (Proceedings of the National Academy of Sciences of the United States of America.

2. Which journals allows users to reuse their material?

1. Journal of Cell Biology

2. BMC Cell Biology

3. Public Library of Science


Lab 2

Pubmed

1. Which chromosomes contribute to the nucleolus?

five chromosomes 13, 14, 15, 21, 22 contribute to the nucleolus.


--Mark Hill 08:01, 24 March 2011 (EST) Do you need help with this question?

2. Identify and add a link to your page of a recent cell biology article using confocal microscopy

Figure 9-19. Conventional and confocal fluorescence microscopy compared

Exercise: Look through editing basics, there is a link called project referncing,; step by step to a link to a reference in the Pubmed page.

Lab 3

1. Find the SDS information for chloroform and identify the hazards associated with this chemical.

Chloroform is a carcinogen. This means Absorption through the skin may result in exposure.

The United Nations Number (UN number)is 1888.

This substance had a A Chemical Abstract Service Registry Number (CAS Number: 67-66-3).


The classification of chloroform is

Xn; R22-48/20/22 Xi; R38 Carc. Cat. 3; R40


Description:

Xn : Harmful

R22-48/20/22 are risk phases associated with chloroform.

R22 is Harmful if swallowed.

R23 Toxic by inhalation.

R24 Toxic in contact with skin.

R25 Toxic if swallowed.

R48/20/22 Harmful: danger of serious damage to health by prolonged exposure through inhalation and if swallowed.



2. You will need to upload an image and add it to your page, with the reference and copyright information with the image.

FGF receptor expression pattern in a b3 stage lung.jpg

[1]


References

Lab 4

1. Identify a commercial supplier of an antibody that relates to your group project topic.

Two monoclonal antibodies, bd-17 and bd-24.

[1]

2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria? (Hint - Watch Part 2: Factors Involved in the Intrinsic Pathway of Apoptosis

Gene is located in the nucleus of the cell and is trapped in mitochondria by the mitochondria's outer membrane.


Lab 6

Group 3 graph.JPG

We noticed an increase in "stringed" phenotype and a significant decrease in "pronged" catagory when moving from over expressing Tm4 to control respectively. This shows that over expressing Tm4 increases strutural capabilities.

Work Area

Intro Lab

PNAS


Lab 9

Identify from one of the cell line repositories: a neural cell line and a muscle cell line.

Neural

Homo sapiens (human) 

Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrow

Muscle

Rattus norvegicus (rat) Tissue: skeletal muscle Cell Type: myoblast myoblast


Identify the species and growth conditions for these cell lines.

Neural

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°CStorage

Muscle

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: The myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent.

[2]