User:Z3254433

From CellBiology

Lab Attendance

1. --z3254433 10:35, 10 March 2011 (EST)

2. --z3254433 09:33, 17 March 2011 (EST)

3. --z3254433 09:18, 24 March 2011 (EST)

4. --z3254433 09:37, 31 March 2011 (EST)

5. --z3254433 20:38, 7 April 2011 (EST)

6. --z3254433 09:16, 14 April 2011 (EST)

7. --z3254433 10:11, 21 April 2011 (EST)

9.lab 9 i was here but i forgot to sign on.

10. --z3254433 09:41, 12 May 2011 (EST)

Individual Assessments

Lab 1

1. What are the key cell biology journals?

the journal of cell biology

public library of science

nature journals

trends in cell bioloy

cell


2. Which journal allows reuse of their published content?

Journal of Cell Biology, BMC Cell Biology and Public Library of Science allow reuse of content provided the article is properly cited.


here is some bold text

here is some italic text

internal link



Lab 2

[1]

Pubmed


lab questions

1. Which chromosomes contribute to the nucleolus?

chromosomes 13, 14, 15, 21, 22

2. Identify and add a link to your page of a recent cell biology article using confocal microscopy from the Pubmed database.

An Atlas for Schistosoma mansoni Organs and Life-Cycle Stages Using Cell Type-Specific Markers and Confocal Microscopy.

pubmed link




Lab 3

1.Find the SDS information for chloroform and identify the hazards associated with this chemical.

Potential Acute Health Effects: Hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of inhalation. Slightly hazardous in case of skin contact (permeator).

Potential Chronic Health Effects: CARCINOGENIC EFFECTS: Classified + (Proven.) by NIOSH. Classified A3 (Proven for animal.) by ACGIH, 2B (Possible for human.) by IARC. Classified 2 (Some evidence.) by NTP. MUTAGENIC EFFECTS: Mutagenic for mammalian somatic cells. Mutagenic for bacteria and/or yeast. TERATOGENIC EFFECTS: Not available. DEVELOPMENTAL TOXICITY: Not available. The substance may be toxic to kidneys, liver, heart. Repeated or prolonged exposure to the substance can produce target organs damage.

Links

[http:/msds.chem.ox.ac.uk/CH/chloroform.html sds chloroform1] msds chloroform

2.You ll need to upload an image and add it to your page, with the reference and copyright information with the following.


Red White Blood cells.jpg [1]

A three-dimensional ultrastructural image analysis of a T-lymphocyte (right), a platelet (center) and a red blood cell (left), using a Hitachi S-570 scanning electron microscope (SEM) equipped with a GW Backscatter Detector.

Image Reference



Lab 4

1. Identify a commercial supplier of an antibody that relates to your group project topic.

biocompare can provide:

  • Mouse Anti- Human ZO1 tight junction protein Antibody, Unconjugated, Monoclonal, Clone mAbcam 61357
  • Rabbit Anti- Human ZO1 tight junction protein Antibody, Unconjugated, Polyclonal

biocomapre


2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria? (Hint - Watch Part 2: Factors Involved in the Intrinsic Pathway of Apoptosis

  • the CYCS gene that encodes cytochrome C is located in chromosome 7.
  • the protein cytochrome C is keep trapped by the outer mitochondrial membrane.



Lab 6

Lab 6 table.JPG

Q 1) what are the changes in phenotypes that you observe between group A and group B?

A =Fan B = Broken Fan C = Stumped D =Pronged E = Stringed F = pyogenic Group a seems to have a greater number of phenotype C and D.


Q 2) how does Tm4 mediate these changes?

other observations with regard to morphology?



Lab 9

1. Identify from one of the cell line repositories: a neural cell line and a muscle cell line. neural cell line:

HUV-EC-C

muscle cell line: G-8


2. Identify the species and growth conditions for these cell lines.

neural cell line: species: Homo sapiens (human)

growth conditions;

1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37°C.

muscle cell: species; Mus musculus (mouse)

growth condition;

Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.