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Revision as of 20:25, 20 April 2011 by Z3253348 (talk | contribs) (→‎Lab 6)

Lab Attendance

Lab 1 --z3253348 10:43, 10 March 2011 (EST)

Lab 2 --z3253348 09:01, 17 March 2011 (EST)

Lab 3 --z3253348 08:54, 24 March 2011 (EST)

Lab 4 --z3253348 08:47, 31 March 2011 (EST)

Lab 5 --z3253348 09:07, 7 April 2011 (EST)

Lab 6 --z3253348 08:54, 14 April 2011 (EST)

Individual Assessments

Lab 1

1. What are the key cell biology journals?

The Journal of Cell Biology


Public Library of Science

Nature Cell Biology

BMC Cell Biology

2. Which journals allow reuse of their published content?

Public Library of Science

The Journal of Cell Biology

BMC Cell Biology

The articles must be properly cited

Lab 2

1. Which chromosomes contribute to the nucleolus?

The nucleolus, located within the nucleus is an important organelle as it is involved in ribosomal RNA transcription and is the site for ribosome assembly. It is formed around nucleolar organisers which involve repeated rRNA genes.In humans, chromosomes 13, 14, 15, 21 and 22 are found which contain rRNA genes contributing to the nucleolus and it's function. Nucleolus Link

2.Identify and add a link to your page of a recent cell biology article using confocal microscopy from the Pubmed database.

An Atlas for Schistosoma mansoni Organs and Life-Cycle Stages Using Cell Type-Specific Markers and Confocal Microscopy

Lab 3

1. Find the SDS information for chloroform and identify the hazards associated with this chemical

MSDS Chloroform

Acute Health Effects:

  • Irritant to skin, eyes and lungs
  • Harmful if ingested or inhaled

Chronic Health Effects:

  • Carcinogenic effects
  • Mutagenic for mammalian somatic cells.
  • Repeated exposure can cause organ damage. Toxic to kidneys, liver and heart.

2. You will need to upload an image and add it to your page, with the reference and copyright information with the image.

p38 MAPK localises to cilia in S. mansoni miracidia

p38 MAPK localises to cilia in S. mansoni miracidia.[1]

Image Reference

  1. <pubmed>21269498</pubmed>

Lab 4

1. Identify a commercial supplier of an antibody that relates to your group project topic.

Labome – The World of Laboratories provides information from antibody suppliers.[1]

Labome provides information on GJB6 Antibody (N-term) - Purified Rabbit Polyclonal Antibody (Pab) and Abgent is a supplier of this antibody [2] This antibody which is found in rabbits reacts with humans and its main use is in immunohistochemistry.

2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria? (Hint - Watch Part 2: Factors Involved in the Intrinsic Pathway of Apoptosis

The CYCS gene encodes for Cytocrome C and is located on chromosome 7. This protein is located on the inner mitochondrial membrane and plays a key role in the electron transport chain. Cytocrome C is maintained within the mitochondria due to the outer membrane and through its attachment to the membrane assisted by cardiolipin. Apotosis occurs when it is released from the membrane due to its dissociation from cardiolipin, followed by the outer mitochondrial membrane becoming more permeable. [3]

Lab 5

Knockout Cell Work Sheet

Lab 6

Phenotype Graph A.PNG

A = Fan

B = Broken fan

C = Stumped

D = Pronged

E = Stringed

F = Pygnotic

1. What are the changes in phenotype that you observed between Group A and Group B in your table?

Phenotype Fan - Tm4 group reduced by about 11%

Phenotype Broken Fan - Tm4 group is reduced by about 30%

Phenotype Stumped - Tm4 group express an increase of about 17%

Phenotype Pronged - Tm4 group show 26% increase

Phentype Stringed - Tm4 show only slight increase of about 3%

Phenotype pygontic - Tm4 only show very slight decrease about 1%

Most common phenotype for Tm4 was stumped and pronged and most common phenotype for the control was fan and broken fan

2. What are the changes in phenotype that you observed between Group A and Group B in table 2?

Phenotype A - Tm4

-increased branching thus more connections formed

-more rounded cell body

-shorter and wider

-stringed and pronged phenotpyes

Phenotype B - Control

-decreased branching tus decreased connections

-flatter cell body

-thinner and longer

-stringed phenotype

2. How does Tm4 mediate these changes?

Tropomyosins are a type of actin binding protein and are found in both muscle and non muscle cells. In non muscle cells many tropomyosin isoforms are present, where they play an important role in the cytoskeleton and functional properties of the actin filaments.[4] This experiment examines the isoform Tm4. Tm4 is expressed embryonically and was present in the growth cones of cultured neurons and is present where there is neurite growth. Thus TM-4 plays a vital role in neurite development and synaptic plasticity.[5] This is reflected in our results where Group A, the Tm4 group was found have an increased number of neurites, increased branching and more overlapping cells. This allows more connections to be formed assisting interaction and development. In contrast group B, the control group had fewer interactions seen with less branching and less overlapping of neurites. Thus, Tm4 is responsible for these phenotypic changes where this isoform mediates increased interactions between cells.

Work Area

Lab 1

Here is some bold text

Here is some italic text

Internal Link: First Lab

External Link: Journal of Cell Biology

Lab 2

Lab Three


Reference List