From CellBiology

Lab Attendance

Lab 1 --z3253348 10:43, 10 March 2011 (EST)

Lab 2 --z3253348 09:01, 17 March 2011 (EST)

Lab 3 --z3253348 08:54, 24 March 2011 (EST)

Lab 4 --z3253348 08:47, 31 March 2011 (EST)

Lab 5 --z3253348 09:07, 7 April 2011 (EST)

Individual Assessments

Lab 1

1. What are the key cell biology journals?

The Journal of Cell Biology


Public Library of Science

Nature Cell Biology

BMC Cell Biology

2. Which journals allow reuse of their published content?

Public Library of Science

The Journal of Cell Biology

BMC Cell Biology

The articles must be properly cited

Lab 2

1. Which chromosomes contribute to the nucleolus?

The nucleolus, located within the nucleus is an important organelle as it is involved in ribosomal RNA transcription and is the site for ribosome assembly. It is formed around nucleolar organisers which involve repeated rRNA genes.In humans, chromosomes 13, 14, 15, 21 and 22 are found which contain rRNA genes contributing to the nucleolus and it's function. Nucleolus Link

2.Identify and add a link to your page of a recent cell biology article using confocal microscopy from the Pubmed database.

An Atlas for Schistosoma mansoni Organs and Life-Cycle Stages Using Cell Type-Specific Markers and Confocal Microscopy

Lab 3

1. Find the SDS information for chloroform and identify the hazards associated with this chemical

MSDS Chloroform

Acute Health Effects:

  • Irritant to skin, eyes and lungs
  • Harmful if ingested or inhaled

Chronic Health Effects:

  • Carcinogenic effects
  • Mutagenic for mammalian somatic cells.
  • Repeated exposure can cause organ damage. Toxic to kidneys, liver and heart.

2. You will need to upload an image and add it to your page, with the reference and copyright information with the image.

p38 MAPK localises to cilia in S. mansoni miracidia

p38 MAPK localises to cilia in S. mansoni miracidia.[1]

Image Reference

  1. <pubmed>21269498</pubmed>

Lab 4

1. Identify a commercial supplier of an antibody that relates to your group project topic.

Labome – The World of Laboratories provides information from antibody suppliers.[1]

Labome provides information on GJB6 Antibody (N-term) - Purified Rabbit Polyclonal Antibody (Pab) and Abgent is a supplier of this antibody [2] This antibody which is found in rabbits reacts with humans and its main use is in immunohistochemistry.

2. In mitochondria, where is the gene located that encode Cytochrome C and what keeps this protein trapped within the mitochondria? (Hint - Watch Part 2: Factors Involved in the Intrinsic Pathway of Apoptosis

The CYCS gene encodes for Cytocrome C and is located on chromosome 7. This protein is located on the inner mitochondrial membrane and plays a key role in the electron transport chain. Cytocrome C is maintained within the mitochondria due to the outer membrane and through its attachment to the membrane assisted by cardiolipin. When apotosis beings it is released from the membrane due to its dissociation from cardiolipin, followed by the outer mitochondrial membrane becoming more permeable. [3]

Work Area

Lab 1

Here is some bold text

Here is some italic text

Internal Link: First Lab

External Link: Journal of Cell Biology

Lab 2

Lab Three


Reference List