User:Group 7 Project

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  • Just had a read about the application of Subcell.Fract. and I would prefer to do this assignment on the mAb's. mAb's is an important tool in biochem., and med! I think there would be alot of info out there, as you said David, because of it's wide application. --Begum Sonmez 08:55, 23 March 2010 (UTC)
  • Hey, I love the topics I have to say! David, I agree with you, Mass Spec. screams out 'electromagnetic rays'! even though all these topics are interesting, we can only choose one. So we'll leave out the Mass Spec. technique. I'm liking the mAb's one. We'll finalise our topic tomorrow than. --Begum Sonmez 08:47, 23 March 2010 (UTC)
  • Hello Group 7, just wanted to say sorry for the late reply/post. I had a quick read of the links provided for subcelullar fractionation and thought it would be interesting and would provide a good amount of information to write (type) about. The 3D cell and tissue patterning topic that I was telling you about, I think isn't the best choice. So that rules that topic out. I'd like to find out more about mass spectrometry though, identify any molecule in a cell? Cool! Talk tomorrow than. --Begum Sonmez 10:09, 22 March 2010 (UTC)

Hey guys,

My option is subcellular fractionation, which I read from one of the books online. Here is the link:

Ill do some more research tomorrow morning and post the links if i find anything.

--Paula Ordonez 09:26, 21 March 2010 (UTC)

--Paula Ordonez 04:29, 22 March 2010 (UTC)

--Paula Ordonez 04:45, 22 March 2010 (UTC)

Hey guys,

I've been having a look through the MBoC textbook and so far there's a few interesting looking topics that other groups haven't claimed:

-Producing monoclonal antibodies using hybridoma cell lines

-mass spectrometry to identify any molecule in a cell

-Gene knockout/knockin

-Reporter genes and in situ hybridisation- tracking gene expression.

Sorry I haven't been able to look at these topics properly but tomorrow or Wedesday morning at the latest I'll have a more in depth look at one or two and post some links.

--David Williamson 09:17, 22 March 2010 (UTC)

Hey guys,

More detail on some of the topics I mentioned:

Producing monoclonal antibodies (mAb's) using hybridoma cell lines

  • produce a single type of antibody from specially modified B-lymphoctytes
  • combine two cells so you end up with two nuclei (B-lymphocyte for ability to make antibodies, tumor cell for ability to live indefinitely in culture) in each cell. This can be done using inactivated viruses or chemicals, so membranes fuse. Fused cells are then selected for with special media.
  • applications include diagnosis and treatment of diseases like rheumatoid arthritis and cancer
  • can localise any protein, follow its movement, and purify that protein to study it further using the resulting mAb's.
  • looks like a pretty involved technique with wide ranging applications- so to me it looks like a challenging topic to do but one that gives us plenty to talk about.
  • Textbook info- mAb's and hybridoma cell lines

Mass Spec

  • as mentioned on 'bones' week after week!
  • uses the fact that charged particles have very precise dynamics in electrical/magnetic fields in vacuum
  • separates ions by mass:charge ratio
  • proteins cleaved into peptides, mixed with organic acid, dried, blasted with laser, forming ionized peptides. These fly towards a detector, how long it takes them to get there depends on their mass and charge.
  • the results are run through a database of known proteins to find what it is
  • can also be used to derive the aa sequence of proteins, similar to PCR for DNA.
  • can use to look at protein-protein interactions, post translational modification etc
  • this would be an option for a technique but it sounds like a bit too much physics in there for me!
  • textbook info- mass spec

I think we go with either subcellular fractionation of the hybridoma cell lines one. From having a quick look I get the sense that subcellular fractionation would be more straightforward but mAb's and hybridoma cell lines gives us the advantage of having a bit more to talk about- which could be a good thing as all three of us have to contribute... What do you guys reckon?

--David Williamson 01:32, 23 March 2010 (UTC)

Hey guys,

mAB's sounds good to me :). looks really interesting!