Talk:Group 9 Project - Fluorescent Proteins

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Revision as of 18:56, 27 April 2010 by Z3187802 (talk | contribs)

Sounds fine to me Louisa. Good idea dividing the different fluorescent proteins based on their emission spectra. "What makes a good fluorescent protein" could come under the "advantages" section, this will allow you to describe what good properties the FP has and why this is an advantage. Photoactivatable FPs and the development of infra-red FPs sounds good. See ya at lab, we discuss this more. --Vishnnu Shanmugam 08:55, 27 April 2010 (UTC)

Hi Guys. I've had a look how i'm going to do the "new FP" section, and i have a few different options which i thought i'd run past you. It's looking like the best way to approach it is to look at the variants of GFP and split them into Yellow, Blue, Orange and Rec spectrum categories to discuss the properties, advantages/disadvantages and uses of each. Does this sound like an ok approach? This will probably also need some info on what makes a "good" fluorescent protein- brightness, photostability etc. Do you think this would fit under any of the existing headings?? I have a couple of really good review articles that look specifically at this stuff so it shouldn't be very hard. Also, apart from colour variants there are lots of other types of FPs so i might just focus on photoactivatable FPs and the development of infra-red FPs if that sounds ok?!? Anyway, if you don't get this, then I'll catch you both in the lab tomorrow. --Louisa Frew 03:58, 27 April 2010 (UTC)

Hi! I had a look and completely agree we were a little over enthusiastic with the number of subtopics! Given the changes, we should defintately sort out a new distribution of sections in the lab today so we can get properly started. --Louisa Frew 01:57, 31 March 2010 (UTC)

Hi folks, I had a look at some of last year's cell biology group projects (can be found on the 2009 student link after clicking on 2010 projects). I propose that we consider revising some of our subtopics as they seem to cover the same thing. Eg. 3)Early/Historical Uses of GFP and 4)History development (GFP). Another is 6)Contemporary/commercially available fluorescent proteins and 7)Uses in current research which once again is the same thing as it is not possible to write about current research without writing about Contemporary/commercially available fluorescent proteins. We probably don't need this many topics but make each one detailed. Example:

  1. Introduction------S
  2. History of fluorescent proteins- this includes all GFP (development,nobel prize, historical uses)-------S
  3. Development of new fluorescent proteins - new proteins since GFP and how they were developed---write detailed about most important fluorescent proteins-----L
  4. Current research - How the new fluorescent proteins are used and to what purpose in research projects today eg. nuclear staining-------V
  5. Advantages of fluorescent proteins-------V
  6. Limitations of fluorescent proteins-------L
  7. References
  8. Links to current research sources (people and organisations)
  9. Glossary

Have a look at the 2009 projects and tell me what you guys think. --Vishnnu Shanmugam 01:31, 30 March 2010 (UTC)

Project outline: (please add suggestions)

  1. Introduction- define fluorescent proteins
  2. The fluorescence process- general explanation L
  3. History- development (GFP)- Nobel Prize etc S
  4. Early/Historical Uses of GFP L
  5. Developments that followed GFP- new proteins (colours, variations etc) S
  6. Contemporary/commercially available fluorescent proteins- different brands, uses etc V
  7. Uses in current research V
  8. Future ??
  9. References
  10. Links to current research sources (people and organisations)
  11. Glossary

Some Subtopics for Fluorescence techniques:

  1. Immunofluorescence
  2. Fluorescence in situ hybridization
  3. Fluorescence Microscopy
  4. Fluorescence trangenesis
  5. Flow Cytometric Fluorescence
  6. Fluorescent marking and labelling
  7. Fluorescent Proteins
  8. Fluorophores

Hello! Fluorescence is still looking like a good option. We've added a few to the list! See you next week. --Louisa Frew 07:00, 17 March 2010 (UTC)3 .

hey i found great articles but 1 of them is german i have an exam soon so ill get on to translating it.