Talk:Group 7 Project - Monoclonal Antibodies

From CellBiology

Peer Evaluation

I really liked the interesting facts section - it was a nice idea and is something a bit different. I could see from the amount of information on the page that you have all done quite a lot of research into Monoclonal antibodies. I liked how the history section was done by decades - it was a different way of looking at the history. One thing you could improve on would be reducing the amount of text on the page - it is kind of daunting and make the page really long.

Group Discussion

Hey guys,

My option is subcellular fractionation, which I read from one of the books online. Here is the link: [1]

Ill do some more research tomorrow morning and post the links if i find anything.

--Paula Ordonez 09:26, 21 March 2010 (UTC)


--Paula Ordonez 04:29, 22 March 2010 (UTC)


--Paula Ordonez 04:45, 22 March 2010 (UTC)

Hey guys,

I've been having a look through the MBoC textbook and so far there's a few interesting looking topics that other groups haven't claimed:

-Producing monoclonal antibodies using hybridoma cell lines

-mass spectrometry to identify any molecule in a cell

-Gene knockout/knockin

-Reporter genes and in situ hybridisation- tracking gene expression.

Sorry I haven't been able to look at these topics properly but tomorrow or Wedesday morning at the latest I'll have a more in depth look at one or two and post some links.

--David Williamson 09:17, 22 March 2010 (UTC)

  • Hello Group 7, just wanted to say sorry for the late reply/post. I had a quick read of the links provided for subcelullar fractionation and thought it would be interesting and would provide a good amount of information to write (type) about. The 3D cell and tissue patterning topic that I was telling you about, I think isn't the best choice. So that rules that topic out. I'd like to find out more about mass spectrometry though, identify any molecule in a cell? Cool! Talk tomorrow than.

--Begum Sonmez 10:09, 22 March 2010 (UTC)

Hey guys,

More detail on some of the topics I mentioned:

Producing monoclonal antibodies (mAb's) using hybridoma cell lines

  • produce a single type of antibody from specially modified B-lymphoctytes
  • combine two cells so you end up with two nuclei (B-lymphocyte for ability to make antibodies, tumor cell for ability to live indefinitely in culture) in each cell. This can be done using inactivated viruses or chemicals, so membranes fuse. Fused cells are then selected for with special media.
  • applications include diagnosis and treatment of diseases like rheumatoid arthritis and cancer
  • can localise any protein, follow its movement, and purify that protein to study it further using the resulting mAb's.
  • looks like a pretty involved technique with wide ranging applications- so to me it looks like a challenging topic to do but one that gives us plenty to talk about.
  • Textbook info- mAb's and hybridoma cell lines

Mass Spec

  • as mentioned on 'bones' week after week!
  • uses the fact that charged particles have very precise dynamics in electrical/magnetic fields in vacuum
  • separates ions by mass:charge ratio
  • proteins cleaved into peptides, mixed with organic acid, dried, blasted with laser, forming ionized peptides. These fly towards a detector, how long it takes them to get there depends on their mass and charge.
  • the results are run through a database of known proteins to find what it is
  • can also be used to derive the aa sequence of proteins, similar to PCR for DNA.
  • can use to look at protein-protein interactions, post translational modification etc
  • this would be an option for a technique but it sounds like a bit too much physics in there for me!
  • textbook info- mass spec

I think we go with either subcellular fractionation of the hybridoma cell lines one. From having a quick look I get the sense that subcellular fractionation would be more straightforward but mAb's and hybridoma cell lines gives us the advantage of having a bit more to talk about- which could be a good thing as all three of us have to contribute... What do you guys reckon?

--David Williamson 01:32, 23 March 2010 (UTC)

  • Hey, I love the topics I have to say! David, I agree with you, Mass Spec. screams out 'electromagnetic rays'! even though all these topics are interesting, we can only choose one. So we'll leave out the Mass Spec. technique. I'm liking the mAb's one. We'll finalise our topic tomorrow than.

--Begum Sonmez 08:47, 23 March 2010 (UTC)

  • Just had a read about the application of Subcell.Fract. and I would prefer to do this assignment on the mAb's. mAb's is an important tool in biochem., and med! I think there would be alot of info out there, as you said David, because of it's wide application.

--Begum Sonmez 08:55, 23 March 2010 (UTC)

Hey guys,

mAB's sounds good to me :). looks really interesting!

As per discussion today, for the next week the plan is to have a look at:

  • History & Early Experiments- Paula
  • Methodology-David
  • Applications + Future Direction (Clinical, and Cell Biology Research)-Begum

--David Williamson 06:57, 24 March 2010 (UTC)

Hello. Found this online book through the library website, called 'Making and Using Antibodies : A Practical Handbook'. Thought it would be useful. Click here.

--Begum Sonmez 11:44, 29 March 2010 (UTC)

I like this video. Have a look, maybe we can use it on our page.

--Begum Sonmez 06:05, 31 March 2010 (UTC)

Hello, I thought we could have our sub-headings up on our page, like Mark said today. These are my suggestions:

  • Intro (definition of monoclonal antibodies, general applications outlined...may include a brief summary of the technique?). I read a part of an article today and it mentioned the technique researchers used before Monoclonal antibodies were discovered, which was 'Polyclonal Antisera'. We could introduce our topic by mentioning how ineffective that was compared to monoclonal antibodies. Or it could go under History?
  • Brief History+Some Early experiments (History of the development of the method and the history of the use of the method?)
  • Methodology
  • Limitations of using mAb's (I found info on this!)
  • Present Applications+Future directions/use
  • Glossary
  • References

What do you both think about the order? Any other ideas on the sub-headings? Any other sub-headings?

--Begum Sonmez 10:44, 31 March 2010 (UTC)

Hey, just wanted to ask if we could each have our own reference list at the bottom of the page just for the convenience of it. We could merge it all together later. I also noticed from Embryo that we mark the end of a sentence with a number/symbol (that matches the number/symbol in the refencing list) to remind ourselves to reference. Could each of us use a different method. One of us can use numbers, letters...I think it just avoids confusion. Let me know If I'm getting overboard :)

And we also have to choose a style of referencing as well to stick with. Any ideas? Or we could ask Mark and see what he wants. I'll email him now. Have fun and enjoy your break! --Begum Sonmez 08:51, 1 April 2010 (UTC)

Hello! Hope your break has been fun! Just wanted to let you both know that I'll have the 'mAb's' library book, that I mentioned last week, soon. I think it will be very useful for our project. --Begum Sonmez 09:39, 10 April 2010 (UTC)

heyy yeh i went to the library the other day and there were actually heaps of good books in terms of methodology and clinical applications so definitely alot of information. hope you guys are going well, ive made notes from different books (still have some articles to read) but i havent had time to organise them into a coherent 'history' section. --Paula Ordonez 23:36, 10 April 2010 (UTC)

Great work guys. Begum, those subheadings and what you've put up so far look great. I'm sure as we go we'll find the need to add and change headings but that looks like a very strong set to start with.

I think Mark mentioned Pubmed format is his reference format of choice. He uses this in the lectures- it uses Pubmed IDs (PMID) as the in text citation, then has a specific format for writing the full reference out at the end. I'm not sure how this works for books/ other non-journal resources though... Certainly in the mean time it'll be fine to include our reference lists separately at the bottom of the page (so long as all the info's there) as Begum suggested and clean them up into a common format and order later as Begum suggested. As far as I understand, "Pubmed central" is the public access database, while "Pubmed" is the one that needs to be subscribed to- so it will have additional full text articles. To access pubmed go to the library>sirius>find resource>the letter p> sign in, then you'll get pubmed references from your search (I think...).

I've put some applications I've come across in the applications section under "other"- which we may or may not end up using (there will be endless applicaions so there's no way we can go into detail about all of them).

I'm also thinking the older methods like polyclonal antibody production by injecting a protein into a live animal should maybe go in the history section rather than methodology? so I renamed methodology to something that's more specific in just the methodology used now to produce mAbs for now... Of course feel free to make alternate suggestions or disagree with any of this! I started the method section but haven't gotten far... --David Williamson 14:23, 13 April 2010 (UTC)

I loved the Intro to Methodology, very interesting. Nice work David. Hopefully I'll have some more applications up soon. --Begum Sonmez 13:10, 17 April 2010 (UTC)

Hey Paula, here is some history material I came across that might be useful to you:

  • 1975: Kohler and MilsteinIn published their seminal manuscript on hybridoma technology enabling the production of mouse monoclonal antibodies (You might have already come across this already). I have an article with me in hard-copy that summarises what they did in like a paragraph. If you like I can bring it for you?
  • mAbs have been used to define distinct subpopulations of intrathymic lymphocytes at different stages of maturation and their alteration in disease (Reinherz EL, Kung PC, Goldstein G, et al:Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes on leukemia lymphoblasts of T-cell lineage. Proc Natl Acad Sci USA 1980;77:1588-1592)
  • use in organ transplantation. Acute kidney graft rejection has been reversed by in vivo administration of a murine monoclonal antibody that binds to a surface antigen found on the majority of mature cells (Cosimi AB, Colvin RB, Burton RC, et al: Use of monoclonal antibodies to T-cell subsets for immunologic monitoring and treatment in recipients of renal allografts. N Engl J Med 1981;305:308-314.
  • used to preventgraft-v-host disease complicating allogeneic bone marrow transplantation.
  • Monoclonal antibodies have been used to detect tumorassociated antigens on human melanoma, colorectal, neuroblastoma,lung, breast, pancreatic, teratoma,glial, leukemia, and lymphoma neoplastic cells(Mitchell MS, Oettgen HF: Hybridomas in the diagnosis and treatment of cancer, in Mitchell MS, Oettgen HF (eds): Progress in Cancer Research and Therapy. New York, Raven Press, 1982, vol 21, pp 1-264).

All of these are from this one review article. I'll have it with me this Tuesday. --Begum Sonmez 08:42, 18 April 2010 (UTC)

We need pictures!!! It's hard when so much of the great photos out there are copyrighted... --Begum Sonmez 22:33, 20 April 2010 (UTC)

Hey David, I added 'The Monoclonal Antibodies South Australia (MAbSA) facility at the University of Adelaide is an example of a facility that develops hybridoma cell lines and purifies mAbs. ' AND a link to a video to your section. I thought it would be best to have it in your section. Feel free to take it out. Here is the link to an alternative photo to the one in your section showing hybridoma method: [4]. You can choose between the two.

And, please, if there's something that's not nice that I've done or If I have too much/too little information, please let me know. Thanks!

--Begum Sonmez 04:12, 1 May 2010 (UTC)

Hey guys, looks good I think it's coming along nicely. Are we still all happy with the topic allocations? I thought my section would be a bit limited but have found there's actually a lot more depth I can go in to, particularly regarding the different forms of mAbs (humanised etc) as you both pointed out to me. I'm aiming to have my section fairly well covered, and at least one self-made picture up by Wednesday's lab. If we are able to evaluate each others' sections before wednesday that would be good but I know I'm not yet that happy with mine yet, how about you guys? --David Williamson 03:04, 3 May 2010 (UTC)

Hey guys,

Im currently editing mine to try and make it more student-friendly i.e. not a big chunk of writing. And i am still organising the succession of events with the development of monoclonal antibody production and types. I will also add to the glossary with a few terms that students may not understand and any interesting facts I come across. Also, did you want me to do the merging of the references, i dont mind. I should have all of this up by tonight. Give me any suggestions on how you think I could improve it. Love the pictures btw!

--Paula Ordonez 06:11, 3 May 2010 (UTC)

Hey, hope you both get this message. I was going to combine our references together...can I? And Paula, I love the image of history you uploaded, but I think it would look better at the beginning, or else we'll keep having the problem with it overlapping with the methodology section. --Begum Sonmez 02:45, 5 May 2010 (UTC)

I think methodology is close now... main things I still want to add are some details of how mAbs can be modified (combined to be fluorescent etc), and a little more detail on how you screen for and isolate a specific antibody. References Definitely need consolidating at some stage- it's just a matter of whether we do it now or at the very end? and I haven't actually found Pubmed IDs for my journals- but I'm more than happy to go through and do this later on. --David Williamson 03:54, 5 May 2010 (UTC)

Hey Begum, by all means you can do the referencing...but yeh definitely at the end when we have finished putting everything that we want in our sections. I will work on the glossary after this peer-review week, as i really like the idea of linking the words in the glossary to the words in the text.

--Paula Ordonez 07:21, 5 May 2010 (UTC)