Talk:Group 4 Project - Cell Culture

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Revision as of 08:57, 12 May 2010 by Z3187043 (talk | contribs)

--Thomas Fox 21:57, 11 May 2010 (UTC) Great work on the project guys, i now have an even better understanding of what is behind the cell culture method. few pointers:

  • Make the hand drawn diagram bigger so you can see it clearer.
  • Add a few more references in certain parts, such as the intro

Overall well done

--z3254509 21:32, 11 May 2010 (UTC)

Overall, great job on the project. I liked the history part was done by the key players, that is an interesting take that seperates your page from the others. Some points for improvement:

  • The references look much too short, need to bump that up a bit.
  • The hand drawn picture could be done a little better, I feel that it doesn't really add much to the page. maybe use your paint skills, or add another picture.

--z3269335 10:53, 11 May 2010 (UTC)

Hey guys,

Your group has done a great job and effort is seen to been put into the project. I have been to the lab for cell culture, however, I think your project maybe even more interesting than what is taught in the lab. The language used in your project could be easily understood. Also, it is also nice to see all the colourful pictures. Additionally, it is a good idea to include helpful links in your project, so that it would be more convenient for the readers to read further materials in relation to cell culture.

The only thing that you may improve may be: instead of drawing the "self=drawn" diagram with hands, it maybe more professional to draw it using the computer.

Overall, you guys have done a great job. Keep it up :)

--Dougall Norris 01:05, 11 May 2010 (UTC)

I think this page is really good, but with a little more work it could be up with the best. Most of the page, excepting methodology, really needed references to back up the points presented. A few of the images require permissions as well. I really like the extent of your information and the lay out, well done. Here are my suggestions:

  • I think the intro could have a few more references.
  • I dont think the history part requires pictures to make it easier to understand, knowing what the scientist looks like doesnt add to my understanding. I would possibly turn it into a timeline, although i dont think this is imperative. I think you should still reference the history more though, to add credibiity.
  • I didnt really understand why potential cell culture problems was in methodology, i think it should definitely be put with Limitations. I like how well referenced the methodology section was though.
  • Applications were set out well and its good to see how many applications were covered, yet it is in dire need of references, there are studies that would be easily found on sirius that would back up the use of cell cultures, so i think this really needs to be done. The pictures are good, but need to be referenced as well, including permissions.
  • Limitations was an important, concise section, but it needs references too as there werent any.
  • Current research and advances is especially important, yet too needs to be referenced.

--Jin Lee 12:45, 10 May 2010 (UTC)Hello Group4~ Firstly great work guys~ ! This page was well organised into each subheadings and the information is evenly distributed. With Methodology and Application part, information was very concise and helpful with pictures. awesome work!

Here is some of my suggestions for you page:

  • complete the Glossary sections.
  • all the images should be properly referenced and include the copyright disclosure.
  • the reference list was a bit short for the amount of text you have uploaded on your page. I found Application section was not properly referenced.

Overall, This is great page! inclusion of Helpful Links was also good idea. Well done!


--Jae Choi 12:34, 10 May 2010 (UTC) Hi, Great project you have made.

  • I like the history part introducing only the main scientists. But if you add a timeline or some pictures, it would be improved. Cheers.


--z3178608 11:24, 10 May 2010 (UTC) Hello Group 4

The project page is extensive in terms on the knowledge about cell cultures. There are several points that I wished to list out after reading the web-page.

  • The introduction gives a excellent brief outline of the contents in the web-page. However, it could be improved by making it more concise.
  • The history include the significant figures whom contribute to the development of cell culture, which makes it more interesting.
  • Informative methodology section that highlight the steps in cell culture as well as details like growth media in culturing.
  • "Potential cell culture problems" could probably be classified under another heading, instead of being a sub-heading under methodology.
  • Well-used pictures in the application section
  • It would be ideal if your group can include more self-drawn diagrams to illustrate the methodology.

Overall, the project is interesting and informative. Well done!

--Joseph Chuk 07:06, 10 May 2010 (UTC) Your project is intersting and I have leanrt a lot about cell culture. The history is very interesting since the scientists are clearly described. I also like the point form you made in the limitation part because it is clear and easy to understand. However, I think the application part should mention the plant tissue culture first, while the point forms should mention last with a subheading 'other applications'. Overall well done!


Good Job group 4. The project looks well thought out, here are some points I thought might be useful: --Vishnnu Shanmugam 04:51, 10 May 2010 (UTC)

  • All images should contain a web link to the source and also copyright information. Some images such as “small forest plants” have no copyright info, others such as “Tissue culture growth medium” have no link to original sorce.
  • Review of the references as there are too few for the amount of text there is. Entire paragraphs should have more than 1 reference. Some have no referencing such as “Current Research & Advances”
  • Excellent glossary
  • Excellent flow chart diagram
  • A time line for the major historical landmarks of Cell Culture would be useful.
  • Like the how you have separated the limitations sections and used point form. Succinct!
  • Try to explain the technique in detail more as the methodology section: does not explain how the cells are isolated, only briefly mentions the growth media and the conditions needed to proliferate. Ie. No mention of ideal conditions temperature 37 degrees, neutral pH, CO2 buffering ect.


Hi Group 4! The page is well laid out, and the structure is well thought out and easy to follow. The student-drawn diagram is a good resource, but I think it would be even better if it was a summary of the procedure desribed in writing first. Although you have addressed aspects such as the harvesting and growth media, there is a noticeable absence of a general procedure, but that's just my take on it. I'm probably biased as a medical science student, but it seems a shame to limit the applications of cell culture techniques to just plant tissue culture. If you do want to leave it like that, i'd consider changing the "applications" heading to "plant tissue culture" or something similar. Also, you could try combining this section with the "current research and advances" section so it's a broader coverage. Your glossary and helpful links are really good, as is your choice of subheadings (not too few or too many). Maybe you haven't added them all yet, but I feel like you need quite a few more references to bring it up to a higher standard. All-in-all, you should end up with a great project! --Louisa Frew 13:54, 8 May 2010 (UTC)


Hey guys. few points to ponder:

  • Very easy to follow
  • The history section could make use of bolded dates or something so they stand out and the reader can chronologically order them
  • The diagrams are plentiful and relevant
  • The in-text references are only really there for methodology. Possibly more references needed overall.

--z3253199 06:15, 7 May 2010 (UTC)


--Katiana Shaw 05:26, 7 May 2010 (UTC)

Hey Group 4 - You have put together a great project on cell culture, which is both easy to understand and informative. Just a few thoughts:

  • I like the detail you included in the history, yet apart from mentioning the US congress in 2006, the rest of the history only goes up to the 1950's. I may be ignorant, forgive me if I am, but surely there would have been some breakthroughs or modernisation of the process since then. I would like to see these included.
  • I think there are maybe a few references missing throughout the text.
  • I particularly like the inclusion of the helpful links - this is a great idea!
  • In your applications section you could maybe include a few links to papers or journals that would allow people who are interested to follow up.

Overall, this really is a good project, reads really well and the pictures compliment the text well. Good work!


--David Williamson 04:44, 7 May 2010 (UTC)

  • I like the layout and headings, but I wonder if ‘potential cell culture problems’ could be merged with ‘limitations’?
  • I think the applications, limitations, current research and advances sections need to be referenced? And some other sections seem like they need more citations...
  • I like the introduction as a good clear summary of the main points.
  • Helpful links is a great idea and the glossary is handy.
  • I think the history section could be clearer if it was arranged chronologically or possibly chronologically for subtopics?
  • I think this page is well written- explained appropriately for people at our level, and manages to keep readers aware of the big picture rather than getting bogged down in details.

--Begum Sonmez 12:11, 6 May 2010 (UTC)

Hello Group 4! I have to say, overall, your project page looked clean, and I did not find much to ‘critic’ about.

  • I prefer if the introduction began with a general statement of Cell Culture, though this is mentioned at an appropriate time later on in the paragraph. Personally, I loved the introduction, it was understandable and clear. The content within the intro has a flow. One change: Divide the intro into 2 (Cell cultures consist of primary cultures, semi continuous cell cultures and continuous cell cultures. (POSSIBLY DIVIDE HERE) The process of cell culture consists of the isolation of cells…)
  • History: If you could find photos of Alexis Carrel AND Charles Lindbergh, it will complete the history section.
  • Methodology: Good use of subheadings and amount of information. The Student-drawn Image helped me understand the method better.
  • From Applications onwards, there seems to me to be a lack of referencing. Be sure to add any references to avoid plagiarism. On the other hand, these sections appeared neat and clear which made everything easier for me to read. It was almost fun!

Clean job Group 4!


--z3256682 06:44, 6 May 2010 (UTC) Hi,

Very detailed, and your drawn diagram was very helpful in understanding how cell cultures are used. However I have just a few suggestions:

- Timeline: next to the three major figures you've selected, put the year period they belong to eg. "Ross Granville Harrison" becomes "1907-1910: Ross Granville Harrison" etc., so the reader can quickly identify the important figure from each period (this is NOT a major issue, just a suggestion).

In the first paragraph for methodology, possibly put primary, semicontinuous and continuous as dotpoints with the relevent description,

eg. cell cultures are divided into three general classes, which are differentiated by where the cells were taken from:

  • Primary cell cultures: blahah
  • Semicontinuous cell cultures: blaah

etc (honestly you have a lot of relevant information, so you want to make it easy on your reader's eyes, as well as break it down into short understandable bites :) ).

Your applications section is interesting (ie. large amount of stuff you can do with cell culture), but needs proper citations to established research/review papers for the ones you've listed.

Also, I realise you've included it very comprehensively in your current research/advances bit, but you could look at more uses for animal/human applications - just to make it a little more relevant instead of only focusing on plants (however the human/plant thing is again a suggestion, and not a big issue, as your coverage could depend on your interests).

Finally, your listed limitations could use some citations, eg. you state it's "ten times more expensive for same quantity of animal tissue" but you don't tell us where you obtained this number from.

Overall, a lot of good work and informative, but just needs a slight tweak. :)


--Emily Wong 10:31, 5 May 2010 (UTC) I can see that you have done quite a lot of work on Cell culture. The things i liked about the page were the history section - selecting only a couple of key people was a good idea as it doesn't overload the reader and the helpful links - that was a really good idea. a way in which you could improve the page is to use some tables - this would eliminate some of the large amounts of text.



Introduction (3220040)

History/Timeline (3220040)

Methodology (3252005)

Cell cultures are divided into 3 general classes:

1. Primary Cultures (Consist of cells derived directly from tissues)

2. Semicontinuous Cell Cultures or Low-Passage Cell Lines (Obtained from subcultures of primary culture)

3. Continous/Immortalised Cell Cultures (Derived from transformed cells that are generally epithelial in origin)--z3252005 09:44, 10 April 2010 (UTC)

Applications (3252231)

Plant tissue culture is used widely in plant science; it also has a number of commercial applications. Applications include:

  • Micropropagation is widely used in forestry and in floriculture. Micropropagation can also be used to conserve rare or endangered plant species.
  • A plant breeder may use tissue culture to screen cells rather than plants for advantageous characters, e.g. herbicide resistance/tolerance.
  • Large-scale growth of plant cells in liquid culture inside bioreactors as a source of secondary products, like recombinant proteins used as biopharmaceuticals.
  • To cross distantly related species by protoplast fusion and regeneration of the novel hybrid.
  • To cross-pollinate distantly related species and then tissue culture the resulting embryo which would otherwise normally die (Embryo Rescue).
  • For production of doubled monoploid (dihaploid) plants from haploid cultures to achieve homozygous lines more rapidly in breeding programmes, usually by treatment with colchicine which causes doubling of the chromosome number.
  • As a tissue for transformation, followed by either short-term testing of genetic constructs or regeneration of transgenic plants.
  • Certain techniques such as meristem tip culture can be used to produce clean plant material from virused stock, such as potatoes and many species of soft fruit.
  • micropropagation using meristem and shoot culture to produce large numbers of identical individuals

Limitations

Current research/advances (everyone)

References (everyone)

  • Willey J.M., Sherwood L.M., & Woolverton C.J. (2008).Prescott, Harley, and Klein's Microbiology (7th ed., pp. 866). New York: McGraw-Hill.


Hey I posted some of the information I found for methodology and put up the reference for the Information in APA form. I wasn't sure which format the references should have been in so if you guys want to use a different referencing style let me know. Sorry guys did have access to the internet for most of the week so I couldn't post up more information.--z3252005 10:16, 10 April 2010 (UTC)


I found an article which lists some common problems that can be encountered during cell culture. The article is [Corning guide for identifying and correcting common cell growth problems]. I was wondering whether I should add this as part of the methodolgy heading or should this be under limitations?--z3252005 10:33, 10 April 2010 (UTC)


  • hey guys sorry i could not come to the lab last week before the semester break coz i was feeling sick. during the break i am sure everyone was very busy and we could not work on cell bio assessment i guess. coz i was very busy wit the mid-session exam and assignments tht r due this week for me. but this week hopefully starting from tomorrow on i am going to work on applications and as soon as i finish this i will continue with the limitations so that we finish everything by the due date. gudluck wit ur studies!! Angama.

Hello guys I was wondering what we are planning to do for the drawing? I was thinking of maybe a simple flowchart of the methodology of cell culture. If you guys have any other ideas post them up so we can decide what to do. Hope you guys have a good weekend.--z3252005 02:31, 18 April 2010 (UTC)

hello, well i think flow chart is a good idea and is very easy to interpret and get the information to the audience quite easily. so i think go ahead wit ur idea and hopefully it is gonna work well. for the drawings i havent searched anything yet because i wanna finish off the info bit first. but i think if u come across a drawing tht relates to the info in ur section go for it upload it. n hopefully we wil help each other to see wat we can come up wit. coz im not sure either. anywayz guys gudluck though!! Angama.


Hey Guys I have almost finished the methodology, but I was wondering if we need a glossary for our page? Just let me know what you guys think.--z3252005 08:39, 26 April 2010 (UTC)

yea definately we need a glossary and also the links in the reference section. angama.

Ill do the glossary, im also working on the timeline & pictures & i wanted to wait until most of the parts were done so i culd include it in the intro --Joanne Raffel 06:24, 28 April 2010 (UTC)

Joanne I found a table for the history and timeline section which may be useful for you. Here is the link Some Landmarks in the Development of Tissue and Cell Culture.--z3252005 13:30, 1 May 2010 (UTC)

Hey Guys I found out how the references should be posted. Project Referencing webpage shows how we should post our references. You guys can use my references to see how to do it as I have fixed up my refernces.--z3252005 03:32, 2 May 2010 (UTC)

Glossary is done. I have also added a helpful links section if you can think of anythin to add, ive included centers focussing on cell culture & pubmed. Hey guys im not sure how u did the references section & i tried to copy what you did but i cant seem to add my stuff without deleting part of the page so im just goin to put my stuff up and we can adjust it properly when i know how you did it. ive also finished my section but i just need to find another picture without breachin copyright laws. Jo.

Hey guys I just posted up the flowchart for cell culture. If you guys have any problems with it let me know.--z3252005 08:38, 3 May 2010 (UTC)

Hey guys I was wondering that you both havnt included any info for the last 2 sections which we are all supposed to add something. I have added bit of info for the last 2 sections so if you guys add some more stuff especially for the current research part it will make our page look complete. Cheers! Angama.

I posted up some info into the current research section, sorry about being late. I just wanted to know if you guys wanted to post up a couple of links for cell culture videos in the helpful links section?? Angama could you put your references up as well, thanks.--z3252005 03:41, 5 May 2010 (UTC)