Talk:Group 3 Project- Immunohistochemistry

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Revision as of 19:05, 5 May 2010 by Z3217043 (talk | contribs)

Hey!

I was thinking about the project and I think to make it easier we should break it up into sections and divide it amongst ourselves. I briefly looked up our topic and apart from the stuff like history and what it is used for, there seems to be a huge number of techniques which fall under this umbrella. One website listed all these techniques like direct method, indirect method, PAP method, ABC method, etc. Maybe should discuss tomorrow how much detail we want to go into without making this epic. Also, thought I should let you know, there has been a death in my family and I will be up north near the Gold Coast from Thursday till Sunday without internet access. If you need to contact me for anything email my zmail cause it gets forwarded to my phone so at least I can stay in contact if need be.

Thanks!

--Katiana Shaw 12:47, 23 March 2010 (UTC)


This is what was we have decided to do for our project, though I should put it into writing on this site.

Basic outline - including umbrella definition and uses. Here is a basic definition. "Immunohistochemistry is the localization of antigens or proteins in tissue sections by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, or colloidal gold." - http://www.ihcworld.com/introduction.htm

History/Timeline - we should include when each of the methods we intend to talk about came into use, etc.

There are heaps of methods used - Choose 9, take 3 each and research what they are (briefly), what they involve, advantages and disadvantages, what research/papers this method has been used in, etc. Methods include: "Blocking, Controls, Direct Method, Indirect Method, PAP Method, ABC Method, LSAB Method, Polymeric Method, CSA Method, Sensitivity Chart" - http://www.ihcworld.com/introduction.htm

Section on current advances/breakthroughs

Also, to compare the methods we need to find a way to compare them in an easily understandable way, ie. table with ticks and crosses.

Lets spend the rest of this week deciding who gets what methods and post any information we find up here.

--Katiana Shaw 06:46, 31 March 2010 (UTC)


Hey guys, found ths site that lists information about the different methods. http://www.novusbio.com/support/how-to/perform-immunohistochemistry.html I was thinking I'll just take the first three methods (direct, indirect and PAP) and you two can decide what you want to do. I've already got a fair bit of information and have started writing bits of it. Would be really good if you guys can post on here what you have done just so that we're all on the same page with where this is going. Thanks! --Katiana Shaw 02:48, 7 April 2010 (UTC)

Found a basic timeline on the history; struggling to find more info on it.

http://anatomy.yonsei.ac.kr/slide/res/IHC99/tsld004.htm

This site is a google timeline. It has links to papers that have used immunohistochemisty in their research. Worth a look.

http://www.google.com.au/search?q=history+of+immunohistochemistry&hl=en&tbs=tl:1&tbo=u&ei=uG_BS9v-L47o7APVxqDCCQ&sa=X&oi=timeline_result&ct=title&resnum=11&ved=0CC8Q5wIwCg

--z3219308 06:47, 11 April 2010 (UTC)

This is a great page on immunhistochemisty. http://www.mesotheliomaweb.org/immunohistochemistry.htm Gives a great definition and goes into detail about the process.

http://www.ihcworld.com/_intro/ihc-methods.htm This page gives a brief overview of each of the methods. What methods are you two doing?

--z3219308 06:53, 11 April 2010 (UTC)

Hello?? Ok so not sure what you guys are up to but you may notice I added some headings and have started writing the definition and all that. Finishing drafting my methods so will put those up in the next couple days and then we can keep adding to them and hopefully start adding the other methods and some pictures soon. --Katiana Shaw 10:40, 12 April 2010 (UTC)

hi guys, sorry for the extremely late response, been a bit caught up with things lately. ill take the ABC method, LSAB method and polymeric method. ill post up some work within the next week. --Julianna Lam 07:34, 17 April 2010 (UTC)


http://www.piercenet.com/Proteomics/browse.cfm?fldID=F95B91A9-3DC1-4B56-8E8D-59CA044A8BA7 this website has some information about ABC method and is also good because it lists the disadvantages and advantages of this method. --Julianna Lam 12:30, 17 April 2010 (UTC)


hi ive found this site based on the ABC method with 4 really good images explaining how it works. http://www.reactolab.ch/Vector/ABC%20Method.htm --Julianna Lam 09:53, 19 April 2010 (UTC)

Julianna - This whole thing is due by week 8 which is next week. It is being peer-assessed by everyone else and then we can fix it up but it generally has to be completed. Just telling you this cause you weren't there last week and that's when he told us though he had told us earlier in the semester. I think we should aim at having everything done by this weekend so that we can spend monday and tuesday next week fixing it up a little and adding pictures and all that. ALSO we have to include a diagram that we have drawn. Im thinking it might be good to draw a diagram about how immunohistochemistry works in general because once everyone gets that concept it is easier to understand the different methods. Also, you guys might notice that I have included references after the entries that I have done - that is because Mark told us he would show us how to include references so that it automatically made a reference list and I have no idea how to do it. Will ask him tomorrow if he can possibly show us. Jess what methods are you doing? --Katiana Shaw 05:28, 20 April 2010 (UTC)


thanks for letting me know. im writing up the ABC method now. so that section should be done by tonight. the other two methods i will work on during the week. i have no idea on how to do the reference thing either so yeh its probably a good idea to ask him tomorrow. anyways see you in class.

--Julianna Lam 06:43, 20 April 2010 (UTC)

Hey can you both read the process that I wrote up and read a couple of the sites that I had put on this page earlier and see if I have done that right. I'm still not entirely confident I understand the process properly, espcially with all the different methods. Thanks!!! --Katiana Shaw 06:10, 22 April 2010 (UTC)

Julianna - Have you got all your stuff ready to add??? This is getting peer-reviewed tomorrow! --Katiana Shaw 10:07, 27 April 2010 (UTC)

hey, yeh ive got all the information ready in a book that i borrowed. tomorrow i have three- four hours free before lab class and im going to write everything up. has jess got her stuff ready yet?--Julianna Lam 15:03, 27 April 2010 (UTC)

hey i've pretty much finished the intro, the process (though read over it), the history and my three methods. I went ahead and formatted it and I already formatted your advantages and disadvantages Julianna. I hope you dont mind. Basically just copy what I have done for the table and you should be able to copy it ok..took my a while to get the table looking normal and not all over the page. Of course fix any mistakes that you find in mine and i hope its all ok. See you soon! --Katiana Shaw 18:09, 27 April 2010 (UTC)

im having trouble adding pictures in, so ill paste the link onto the discussion page and we'll do it while we're in class, sorry for the late notice !!!--Julianna Lam 02:10, 28 April 2010 (UTC)

http://www.ihcworld.com/_intro/ihc-methods.htm http://www.ncbi.nlm.nih.gov/pmc/articles/PMC500802/pdf/jclinpath00268-0018.pdf --Julianna Lam 04:13, 28 April 2010 (UTC)

reference - diagnostic immunohistochemistry, david dabbs second edition


hi jess, can you please add in your work by next week's due date. either research on other methods used in immunohistochemistry or give some examples of it's applications or current research. thanks. --Julianna Lam 04:05, 29 April 2010 (UTC)

Hey jess, I'm getting a bit concerned that your part hasn't been uploaded yet. Would be really good if you could have it up before Wed so that Julianna and I can at least have a look and we are content as a group with what is being handed in to be peer reviewed. Also Julianna, do you reckon we should add more about uses of our methods? --Katiana Shaw 03:38, 3 May 2010 (UTC)

i think under each method we should give one example of how the method has been used? i'll try do that tonight or if not tomorrow. --Julianna Lam 03:54, 3 May 2010 (UTC)

i hope you're aware that i will be letting mark know that you havent contributed at all to this assignment if nothing is up by wednesday. --Julianna Lam 10:43, 3 May 2010 (UTC)

Ok Julianna, I will look into some examples. Will try to add them tonight, just to add a bit more. I'm worried that Jess wont add stuff... Do we have to do her part if she doesnt? I forgot to ask Mark what to do in this case...I'm trying to get into Masters, I cant get a bad mark for this otherwise my average will come down! --Katiana Shaw 08:21, 4 May 2010 (UTC)

girls, i will have the stuff up by tomorrow morning. I'm really sorry that I have yet to do it but last week I damaged my knee at hockey and its suspected that I'm going to need surgery. The last few days have been filled with doctors appointments and scans and such. I'm on cructhes and in a hell of a lot of pain. I will have my stuff done. It will be fine. I am really sorry it hasn't been put up yet, but it will be. --Jessie Tomkins 08:38, 4 May 2010 (UTC)

i think the length of our assignment is pretty much similiar to the rest of the groups so it should be fine. it would be good to add some examples, ill try find some information today before lab class. we still have a week or two after the peer assessment to fix everything up.--Julianna Lam 01:36, 5 May 2010 (UTC)


http://vet.sagepub.com/content/44/2/137.full --Julianna Lam 02:41, 5 May 2010 (UTC)

http://www.asianaoms.org/toc/v19n2p89.pdf--Julianna Lam 03:20, 5 May 2010 (UTC)

i added a section on sensitivity with a diagram i made. give it a read and tell me what you think? i'm not going to be at the lab today because of the whole crutches/swollen knee thing. can't drive or public transport it so makes getting there a bit hard! --Jessie Tomkins 06:17, 5 May 2010 (UTC)

Jess we have started peer assessment of each others projects. This is due next week. Basically you have to write your assessment of each group on their discussion page. Put your signature and follow it with your comment. NO changes can be made to our page from 5pm TODAY!!!! Anything you want to change or add has to wait until next week AFTER everyone has assessed our page. --Katiana Shaw 07:27, 5 May 2010 (UTC)

thanks for letting me know Katiana! i think i may have put a small amount of stuff up but i'm sure Mark will understand. I also have found a great picture for the CSA sectin, but how do I know if i can use it or not? let me know p.s what did i miss in the lab?--Jessie Tomkins 08:05, 5 May 2010 (UTC)