Talk:Group 3 Project- Immunohistochemistry

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Revision as of 18:34, 17 April 2010 by Z3218146 (talk | contribs)

Hey!

I was thinking about the project and I think to make it easier we should break it up into sections and divide it amongst ourselves. I briefly looked up our topic and apart from the stuff like history and what it is used for, there seems to be a huge number of techniques which fall under this umbrella. One website listed all these techniques like direct method, indirect method, PAP method, ABC method, etc. Maybe should discuss tomorrow how much detail we want to go into without making this epic. Also, thought I should let you know, there has been a death in my family and I will be up north near the Gold Coast from Thursday till Sunday without internet access. If you need to contact me for anything email my zmail cause it gets forwarded to my phone so at least I can stay in contact if need be.

Thanks!

--Katiana Shaw 12:47, 23 March 2010 (UTC)


This is what was we have decided to do for our project, though I should put it into writing on this site.

Basic outline - including umbrella definition and uses. Here is a basic definition. "Immunohistochemistry is the localization of antigens or proteins in tissue sections by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, or colloidal gold." - http://www.ihcworld.com/introduction.htm

History/Timeline - we should include when each of the methods we intend to talk about came into use, etc.

There are heaps of methods used - Choose 9, take 3 each and research what they are (briefly), what they involve, advantages and disadvantages, what research/papers this method has been used in, etc. Methods include: "Blocking, Controls, Direct Method, Indirect Method, PAP Method, ABC Method, LSAB Method, Polymeric Method, CSA Method, Sensitivity Chart" - http://www.ihcworld.com/introduction.htm

Section on current advances/breakthroughs

Also, to compare the methods we need to find a way to compare them in an easily understandable way, ie. table with ticks and crosses.

Lets spend the rest of this week deciding who gets what methods and post any information we find up here.

--Katiana Shaw 06:46, 31 March 2010 (UTC)


Hey guys, found ths site that lists information about the different methods. http://www.novusbio.com/support/how-to/perform-immunohistochemistry.html I was thinking I'll just take the first three methods (direct, indirect and PAP) and you two can decide what you want to do. I've already got a fair bit of information and have started writing bits of it. Would be really good if you guys can post on here what you have done just so that we're all on the same page with where this is going. Thanks! --Katiana Shaw 02:48, 7 April 2010 (UTC)

Found a basic timeline on the history; struggling to find more info on it.

http://anatomy.yonsei.ac.kr/slide/res/IHC99/tsld004.htm

This site is a google timeline. It has links to papers that have used immunohistochemisty in their research. Worth a look.

http://www.google.com.au/search?q=history+of+immunohistochemistry&hl=en&tbs=tl:1&tbo=u&ei=uG_BS9v-L47o7APVxqDCCQ&sa=X&oi=timeline_result&ct=title&resnum=11&ved=0CC8Q5wIwCg

--z3219308 06:47, 11 April 2010 (UTC)

This is a great page on immunhistochemisty. http://www.mesotheliomaweb.org/immunohistochemistry.htm Gives a great definition and goes into detail about the process.

http://www.ihcworld.com/_intro/ihc-methods.htm This page gives a brief overview of each of the methods. What methods are you two doing?

--z3219308 06:53, 11 April 2010 (UTC)

Hello?? Ok so not sure what you guys are up to but you may notice I added some headings and have started writing the definition and all that. Finishing drafting my methods so will put those up in the next couple days and then we can keep adding to them and hopefully start adding the other methods and some pictures soon. --Katiana Shaw 10:40, 12 April 2010 (UTC)

hi guys, sorry for the extremely late response, been a bit caught up with things lately. ill take the ABC method, LSAB method and polymeric method. ill post up some work within the next week. --Julianna Lam 07:34, 17 April 2010 (UTC)