Talk:Group 2 Project - RNA Interference

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Revision as of 15:18, 12 May 2010 by Z3254934 (talk | contribs)

--Shoahaib Karimi 04:18, 12 May 2010 (UTC)I really like the topic you guys chose, your summary diagram is really good at sumarising it all. You seem to have a lot of references but thats good. You could include a picture of your own. The advantage and disadvantage page was good.


--z3252833 03:45, 12 May 2010 (UTC) Hi there! I really liked this page. First, let me compliment you on your student diagram; it looks amazing (and your citation/copyright is all there) so well done! I also really like your use of tables to summarise information. The page has a clear, logical layout of information and headings; the description of the technique flows logically and paragraphs are concise an easy to read. The glossary is comprehensive and for current uses relevant papers have been used. Just make sure that all your pictures are properly referenced – the first two don’t seem to have citations. I actually can’t think of much to say in terms of improvement; I think you’ve done a pretty great job, here. But you deserve some feedback to think about that’s not just “well done!” so I’m just going to nitpick a little and give you a few things to think about. In your picture “Differences between Wild type and RNAi affected coffee leaves]” there’s a square bracket that has no pair and might want to be deleted. Perhaps, instead of that last sentence “Whilst still in process, it opens the doors of what can be achieved…” being under “Future Perspectives”, you could expand it to two or so sentences and put it under “Conclusion”? So well done, Group 2!


--Jessie Tomkins 03:37, 12 May 2010 (UTC) Very well done group 2. I really like how informative and easy to read your page was. You made the concepts easy enough to understand, although putting some more terms in your glossary might help those without a cell biology background to understand better. Also just go back and make sure all your pictures are referenced properly.

--Mari Fushimi 03:26, 12 May 2010 (UTC) Hi group 2 great work! here is my feedback:

  • Key points are described however the layout could be improved by making the headings more obvious in the applications
  • The use of table was good; history was great as it was easy but informative to read; content of some of the applications could have been more thorough although it was short and sweet; the hand drawn diagram is very impressive!! and 'Future perspectives' was a great section to put in.
  • Good amount of references just need to touch how they are used according to wiki guidelines
  • As Katiana says a general outline would be good to add before going into technical mechanisms
  • Overall great work!!

--Samantha Cabrera 01:42, 12 May 2010 (UTC) Ensure that all images are referenced and have their copyright info. Other than that, great content structure. The headings were appropriate - and wow, a citations list! Good effort as well in the student drawn diagram!

--Julianna Lam 01:24, 12 May 2010 (UTC) the page looks really good. great use of pictures, made the page look very interesting. the history section is very thorough and clear. the use of the table listing the advantages and disadvantages are a good idea. although more attention should be paid towards the referencing. overall its a very good job !

--Joanne Raffel 00:00, 12 May 2010 (UTC) Well done on your wiki page. The page seemed like it was just under one heading from an initial glance, maybe you should break it up a bit more by moving the pictures to different sides of the page. The glossary was extremely short, although most of the information provided would be understood by students studying cell biology, those just looking at the page may have a few difficulties. Your pictures were really good, especially the hand drawn image however it was extremely small. There were also a few grammer errors. I think the comparison section should be under a different heading and further up the page, maybe around the introduction.

--Paula Ordonez 23:28, 11 May 2010 (UTC) Hi group 2, This is a really well organised page. I especially liked the fact that you guys started with explaining what RNAi is, which helped me understand the mechanism later on. I like the fact that you have lots of subheadings with a short paragraph under each one, making it appear less daunting. Also the self drawn picture is really clear. Just a few additions to the glossary and the references will make it flawless.


--Erika Unsworth 23:10, 11 May 2010 (UTC) Overall, I'm very very impressed with this page!

  • It was interesting to read
  • It flows very well- great structure!
  • Great use of formatting
  • The student-drawn diagram is amazing- clear & visually appealing!
  • The only down-side is that some referencing is missing e.g. in the mechanism section. Make sure to check over that before final submission!

--z3254509 21:12, 11 May 2010 (UTC)

Nice work, the formatting on this page is really top notch, and adds a lot to the readability and the flow of the page. The use of a table in the advantages section is a real highlight of this. The drawn diagram is also really impressive. Things that could be improved on though:

  • The glossary really seems very short.
  • The referencing (especially for pictures) could be looked over and be better.

--Rosita Pang 09:48, 11 May 2010 (UTC) Hey guys,

As a whole, I think you have done a great job. Your project explain RNA interference technique precisely and clearly. However, since a correct referencing is one of the marking criteria. It would be nice that if your group have include citations and short descriptions for the pictures Z3216273-Petunia-RNAi.png and Z3216273-authors.jpg.

Despite from it, I think your group have done really well and effort is seen to be put on the self- drawn diagram which explained the steps of RNAi Process.

Keep up the good job!

--Thomas Fox 01:43, 11 May 2010 (UTC) Great job guys, love the layout and student designed diagram. Info is clear and to the point. Learned alot.

--Jin Lee 12:06, 10 May 2010 (UTC)Hello Group 2! well done! I really enjoyed your page. Each section is very clear and precise. I really liked student drawn diagram!

Here is some of my suggestion for your page:

  • with mechanisms section, you will need to be work on referencing.
  • with images, check the copyright disclosure.
  • Complete the Glossary section.

Overall, I really liked this page because I had no idea about RNA Interference but it was easy to follow and understandable. Thanks!


--Joseph Chuk 06:48, 10 May 2010 (UTC) Your project is intersting and I have leanrt a lot about RNA Interference. The history, mechanism and application parts are all very precise. You used a table to compare the advantages and disadvantages, which is also very clear. Well done! Try to reference the images if you can because I think it is part of the marking criteria.

--Angama Yaquobi 05:00, 10 May 2010 (UTC)Hi guys I enjoyed reading your webpage and here are my thoughts about your webpage;

  1. The history section was very informative. I like the idea how you guys have given concise and important info about each year which actually helps a reader quickly grasp the main ideas and also doesn’t make them feel uninterested. So well done.
  2. The method section was very clear & the drawn diagrams were very helpful and clear.
  3. However as mentioned by others I think you should work on the referencing and also add more glossary because I am sure there are some words that need definition in glossary. Other than that a very clear and precise project.

Well done group 2. The project looks excellent, here are some points I thought might be useful: --Vishnnu Shanmugam 04:26, 10 May 2010 (UTC)

  • All images should contain a web link to the source and also copyright information.
  • Review of the references as there are no references in the “Mechanisms of RNAi” section.
  • Increase the glossary for words like “cell cycle gene” and “anti-apoptopic gene”.
  • Student drawn diagram summarizing the RNAi Process needs more explanation. “Effects of siRNA transfection on HeLa cells” picture needs more explanation as it currently not supporting the text.
  • Excellent time line, gets straight to the point, succinct!
  • Like the applications section as it goes through some important diseases.
  • Try to make the project more visually appealing by adding more pictures.


--Dougall Norris 01:28, 10 May 2010 (UTC)

Firstly, great intro, there were a few spelling mistakes throughout the page as previously mentioned, one i saw was "RNA silencing or quelling - is an organism''s response" not organisms.

  • The student drawn diagram was CRAAAZY, so well done, and as said referencing on images needed.
  • Awesome history, well referenced too.
  • Mechanisms desperately needs references, but apart from that it was well written and informative.
  • Applications were spot on, it sounded accurate and was well referenced to back it up.
  • Its really good to see the advantages and disadvantages table. Awesome way of setting something simple like that out.
  • The future part was great, but the (MTB Europe Sept 2008) and (Current Issues in Molecular Biology 7:1-6) need to be put into the references, and i don't think the two sentences after ref 29 have to be on new lines.

On the whole, there were just a few trivial issues, its a solid piece, so well done group 2!


--z3252005 04:34, 8 May 2010 (UTC) Hey Group 2. Your page looks great and I like the way you guys structured the page. The headings and subheadings were used well. Some improvements I could suggest for your page include:

  • Your pictures should be referenced and also include the copyright information (eg.Z3216273-Petunia-RNAi.png, Z3216273-authors.jpg and Z3216273-RNAiCoffee.jpg). Your student diagrams is great, but you guys should reference it properly. Look at the editing basics section to see what information should be included with your drawings/images.
  • There are a couple of really minor spelling mistakes which can be easily fixed (eg. "interderon" which i believe is interferon).
  • The Mechanisms of RNAi section needs in-text references just like the other sections.

This is a great page. I liked the layout of the history section and the image used was appropriate. The mechanisms of RNAi section is great especially the diagram but it would have been better if the images had a caption explaining the steps (a simple outline). Splitting the applications section into medicine and biotechnolgy is a great idea. The use of a table for the advantages and disadvantages section is great. Overall a great page.


Hi Guys. Job well done. The processes of RNAi were explained relatively simply with an effective student drawn diagram. The applications section was especially well done. A few areas need cleaning up like referencing pictures and the mechanisms section but apart from that it is a very strong page.--z3253199 06:51, 7 May 2010 (UTC)


--Katiana Shaw 05:00, 7 May 2010 (UTC) Hi Group 2. Here are my thoughts on your project:

  • The history is really excellent - very comprehensive
  • Your student drawn diagram is fantastic - really illustrates the technique!
  • I would have liked to be able to read a general outline of how the technique works before launching into the different mechanisms. Basically just describe what the picture is supposed to be telling us.
  • Some of your pictures need referencing, though that is easily fixed.
  • Some of the text seems as though it should be referenced.

Overall an informative page!


--David Williamson 06:50, 6 May 2010 (UTC)

  • The layout of headings and subheadings are well thought out.
  • Good clarity of expression- well written in a way that is easy for people that aren’t experts to understand.
  • Impressive student drawn diagram!
  • I’m not sure if you need to reference anything in the first 2 paragraphs of “What is RNAi”?
  • I think the mechanisms section needs to be referenced?
  • Something I’m not sure about- in the opening section it mentions that RNAi gene silencing involves inhibition of transcription through cleavage and degradation of mRNA... if mRNA is already present hasn’t transcription already occurred? And won’t cleavage and degradation of the mRNA only prevent the subsequent translation of that mRNA, with transcription occurring independently?
  • The descriptions of ideas behind medical applications is good- but for some diseases it isn’t clear whether RNAi is used clinically or only in development?


--Begum Sonmez 01:42, 6 May 2010 (UTC) Hello Group 2.

  • The introduction was interesting, and the accompanying photo relevant. Although the photo must be properly referenced. Other photos that need referncing are:
    • '2006 Nobel Prize winners Andrew Fire and Craig Mello for their discovery of the RNAi mechanism.'
    • 'Student-drawn diagram. Summary of RNAi Process Diagrammatical representation of the cellular mechanisms of the RNA interference process.' The author must identify herself/himself throught their name or student number.
    • 'Effects of siRNA transfection on HeLa cells. Anti-lamin RNAi transfected cells show very low lamin A/C staining levels in their nucleus.' Any copyright laws for this photo?
    • 'Differences between Wild type and RNAi affected coffee leaves.' Any copyright laws?
  • ‘The timeline of RNA interference is widely accepted to be as follows...’ This sentence tells me that there is a disptue about the timeline of RNA Interference. If so, it would be good to know what the dispute is. If not, you really don’t need the line.
  • Great work on the Mechanisms section. The accompanying illustration was helpful.
  • For the application section, I like how the applications listed under ‘Medicine’ corresponded to the applications listed under.
  • I like the Adv./Diadv. Table.

Nice effort Group 2.


--z3256682 11:20, 5 May 2010 (UTC) Hi, I found your methods very clear (sufficient "scientific" terminology without sacrificing understandability) along with diagram - though as noted by others, referencing in this section could be improved. A possible thing you could expand on is RNAi in cancer treatment - how will RNAi affect current therapy for cancer patients (eg. radiotherapy, chemo, surgery) and are there any currently in clinical trials? (your citation - Milhavet et al. only indicates cultured cell lines and not real application). This isn't an issue, just a suggestion for you since some posts suggest this section is a bit brief. Other than that, clear and precise.

---

First off, the student drawn diagram is awesome- really clear and helpful! The History and Mechanisms sections are also really well done, but the latter does seem to be missing referencing. The applications section is interesting, but i felt like a couple of the subheadings (eg. biotech and functional genomics) were very general and basic. Overall, the project looks really good. Well done! --z3329502 07:53, 5 May 2010 (UTC)


---

z3252340 07:48, 5 May 2010 (UTC) Your page i really good. I found it was just the right length, not too much text but just enough to get across the basic concepts. I particularly like your history section - it was short and sweet but had just the right amount of information (no one wants to be bored by a huge slab of irrelevant material). Referencing of images could be improved, just on a couple of images though. If they are your own work, copyright them and state from what image they are based upon. A description of what the image is about would also help.



Hello I enjoyed reading the webpage. The schematic diagram with the description has enhanced my understanding on the RNAi. You have included a wide coverage of the applications for RNAi. But, just be mindful about the copyright issue when a image from other source is uploaded. Thanks. --z3178608 07:36, 5 May 2010 (UTC)


yes it is working now.

thanks Gabriel. yeh i spent hours for it haha =)

Jae


now that the page is working today..

great job on the pic jae!

--Gabriel Gutnik 00:47, 5 May 2010 (UTC)

oh and latest research shows that rnai is ineffective in malaria work, so i will take out the link for it

http://nar.oxfordjournals.org/cgi/content/abstract/gkp239v1

--Gabriel Gutnik 14:09, 4 May 2010 (UTC)


it's an easy one to draw ourselves, it's all step by step process, so it is a good one to diagram ourselves using a basic model

do it in paint? i can whip it up if you'd like, should be easy --Gabriel Gutnik 14:06, 4 May 2010 (UTC)


hey

as we should put the "diagram drawn by student" within the page, i'd like to put my drawing for the basic mechanism of RNAi instead of the one what i put on before.

have look and please give me a comment.

cheers

Jae


good idea on the glossary, and the page is quite peer orientated anyway

i think we should still stick with the idea of the applications, after all, that is what rnai is used for - i.e. the basis of why it is important.

i'll finish up the applications tonight. there are also some extra touch ups i will take care of - some of the referencing needs to be clarified. --Gabriel Gutnik 02:19, 4 May 2010 (UTC)


Sequence-specific inhibition of small RNA function. Hutvágner G, Simard MJ, Mello CC, Zamore PD. PLoS Biol. 2004 Apr;2(4):E98. Epub 2004 Feb 24. PMID: 15024405

Effective expression of small interfering RNA in human cells. Paul CP, Good PD, Winer I, Engelke DR. Nat Biotechnol. 2002 May;20(5):505-8. PMID: 11981566

Hey i found a really good article

http://www.academicjournals.org/bmbr/PDF/Pdf2006/DEC/Reddy%20et%20al.pdf

Jae


i think we dont need to keep the types of RNAi..

Short interfering RNAs (siRNAs) - siRNA is a short, 20 to 25 base pairs long, double stranded RNA molecule which is involved in the RNAi.

MicroRNAs (miRNAs)

Tiny non-coding RNAs (tncRNAs)

Small modulatory RNA (smRNA)

Jae


hey team. check this site. i think Darren also know this from molecular biology subject. http://www.hhmi.org/biointeractive/rna/rna_interference/01.html

Jae


Oh, hey. sorry i just checked the page now. yeh we all should know the basic consept of RNAi technique. by the way i will be free from 12 to 3 and after the lab.

Jae


OK finally done the page up a bit so we at least have something decent to present tomorrow, most of this information was just from me skimming journals and from my molecular biology notes from last year so this is far from the final product. We should go in much more depth for when the assignment its due. We also need to organise how we're gonna address this thing tomorrow. --Darren Dizon 12:17, 13 April 2010 (UTC)


Hey guys, Sorry for late reply, For tomorrows presentation i think we should all have a basic understanding of RNAi so that we can give a brief report on it. ALso i agree with the choice of headings that Jae has provided us and think we should further discuss which subheadings we will focus on for the assignment, tomorrow. I will upload some summarised points onto the wiki page later tonight

--Darren Dizon 08:35, 13 April 2010 (UTC)

Hi guys.

we should discuss about this project and divide the work because we are going to have a brief presentation for our work in week 5 just after the break.

Jae


i have added the headings for the content of our project.

Jae


Hey team. i found an article The RNAi revolution from Nature Publishing Group which can be a good sample of our project i think.

Here is the reference

Novina C D, Sharp P A. The RNAi revolution. Nature Publishing Group. 2004. 403:161-164.

Jae


for the history/discovery part, should we mention about the unexpected phenomenon observed in transgenic plants experiments?

obiously we should add the research awarded the Nobel Prize in Physiology or Medicine in 2006 "Potent gene silencing effect after injecting double stranded RNA into C. elegans" in the history of RNAi.

Jae