Talk:Group 2 Project - RNA Interference
--Katiana Shaw 05:00, 7 May 2010 (UTC) Hi Group 2. Here are my thoughts on your project:
- The history is really excellent - very comprehensive
- Your student drawn diagram is fantastic - really illustrates the technique!
- I would have liked to be able to read a general outline of how the technique works before launching into the different mechanisms. Basically just describe what the picture is supposed to be telling us.
- Some of your pictures need referencing, though that is easily fixed.
- Some of the text seems as though it should be referenced.
Overall an informative page!
--David Williamson 06:50, 6 May 2010 (UTC)
- The layout of headings and subheadings are well thought out.
- Good clarity of expression- well written in a way that is easy for people that aren’t experts to understand.
- Impressive student drawn diagram!
- I’m not sure if you need to reference anything in the first 2 paragraphs of “What is RNAi”?
- I think the mechanisms section needs to be referenced?
- Something I’m not sure about- in the opening section it mentions that RNAi gene silencing involves inhibition of transcription through cleavage and degradation of mRNA... if mRNA is already present hasn’t transcription already occurred? And won’t cleavage and degradation of the mRNA only prevent the subsequent translation of that mRNA, with transcription occurring independently?
- The descriptions of ideas behind medical applications is good- but for some diseases it isn’t clear whether RNAi is used clinically or only in development?
--Begum Sonmez 01:42, 6 May 2010 (UTC) Hello Group 2.
- The introduction was interesting, and the accompanying photo relevant. Although the photo must be properly referenced. Other photos that need referncing are:
- '2006 Nobel Prize winners Andrew Fire and Craig Mello for their discovery of the RNAi mechanism.'
- 'Student-drawn diagram. Summary of RNAi Process Diagrammatical representation of the cellular mechanisms of the RNA interference process.' The author must identify herself/himself throught their name or student number.
- 'Effects of siRNA transfection on HeLa cells. Anti-lamin RNAi transfected cells show very low lamin A/C staining levels in their nucleus.' Any copyright laws for this photo?
- 'Differences between Wild type and RNAi affected coffee leaves.' Any copyright laws?
- ‘The timeline of RNA interference is widely accepted to be as follows...’ This sentence tells me that there is a disptue about the timeline of RNA Interference. If so, it would be good to know what the dispute is. If not, you really don’t need the line.
- Great work on the Mechanisms section. The accompanying illustration was helpful.
- For the application section, I like how the applications listed under ‘Medicine’ corresponded to the applications listed under.
- I like the Adv./Diadv. Table.
Nice effort Group 2.
--z3256682 11:20, 5 May 2010 (UTC) Hi, I found your methods very clear (sufficient "scientific" terminology without sacrificing understandability) along with diagram - though as noted by others, referencing in this section could be improved. A possible thing you could expand on is RNAi in cancer treatment - how will RNAi affect current therapy for cancer patients (eg. radiotherapy, chemo, surgery) and are there any currently in clinical trials? (your citation - Milhavet et al. only indicates cultured cell lines and not real application). This isn't an issue, just a suggestion for you since some posts suggest this section is a bit brief. Other than that, clear and precise.
First off, the student drawn diagram is awesome- really clear and helpful! The History and Mechanisms sections are also really well done, but the latter does seem to be missing referencing. The applications section is interesting, but i felt like a couple of the subheadings (eg. biotech and functional genomics) were very general and basic. Overall, the project looks really good. Well done! --z3329502 07:53, 5 May 2010 (UTC)
z3252340 07:48, 5 May 2010 (UTC) Your page i really good. I found it was just the right length, not too much text but just enough to get across the basic concepts. I particularly like your history section - it was short and sweet but had just the right amount of information (no one wants to be bored by a huge slab of irrelevant material). Referencing of images could be improved, just on a couple of images though. If they are your own work, copyright them and state from what image they are based upon. A description of what the image is about would also help.
Hello I enjoyed reading the webpage. The schematic diagram with the description has enhanced my understanding on the RNAi. You have included a wide coverage of the applications for RNAi. But, just be mindful about the copyright issue when a image from other source is uploaded. Thanks. --z3178608 07:36, 5 May 2010 (UTC)
yes it is working now.
thanks Gabriel. yeh i spent hours for it haha =)
now that the page is working today..
great job on the pic jae!
--Gabriel Gutnik 00:47, 5 May 2010 (UTC)
oh and latest research shows that rnai is ineffective in malaria work, so i will take out the link for it
--Gabriel Gutnik 14:09, 4 May 2010 (UTC)
it's an easy one to draw ourselves, it's all step by step process, so it is a good one to diagram ourselves using a basic model
do it in paint? i can whip it up if you'd like, should be easy --Gabriel Gutnik 14:06, 4 May 2010 (UTC)
as we should put the "diagram drawn by student" within the page, i'd like to put my drawing for the basic mechanism of RNAi instead of the one what i put on before.
have look and please give me a comment.
good idea on the glossary, and the page is quite peer orientated anyway
i think we should still stick with the idea of the applications, after all, that is what rnai is used for - i.e. the basis of why it is important.
i'll finish up the applications tonight. there are also some extra touch ups i will take care of - some of the referencing needs to be clarified. --Gabriel Gutnik 02:19, 4 May 2010 (UTC)
Sequence-specific inhibition of small RNA function. Hutvágner G, Simard MJ, Mello CC, Zamore PD. PLoS Biol. 2004 Apr;2(4):E98. Epub 2004 Feb 24. PMID: 15024405
Effective expression of small interfering RNA in human cells. Paul CP, Good PD, Winer I, Engelke DR. Nat Biotechnol. 2002 May;20(5):505-8. PMID: 11981566
Hey i found a really good article
i think we dont need to keep the types of RNAi..
Short interfering RNAs (siRNAs) - siRNA is a short, 20 to 25 base pairs long, double stranded RNA molecule which is involved in the RNAi.
Tiny non-coding RNAs (tncRNAs)
Small modulatory RNA (smRNA)
hey team. check this site. i think Darren also know this from molecular biology subject. http://www.hhmi.org/biointeractive/rna/rna_interference/01.html
Oh, hey. sorry i just checked the page now. yeh we all should know the basic consept of RNAi technique. by the way i will be free from 12 to 3 and after the lab.
OK finally done the page up a bit so we at least have something decent to present tomorrow, most of this information was just from me skimming journals and from my molecular biology notes from last year so this is far from the final product. We should go in much more depth for when the assignment its due. We also need to organise how we're gonna address this thing tomorrow. --Darren Dizon 12:17, 13 April 2010 (UTC)
Hey guys, Sorry for late reply, For tomorrows presentation i think we should all have a basic understanding of RNAi so that we can give a brief report on it. ALso i agree with the choice of headings that Jae has provided us and think we should further discuss which subheadings we will focus on for the assignment, tomorrow. I will upload some summarised points onto the wiki page later tonight
--Darren Dizon 08:35, 13 April 2010 (UTC)
we should discuss about this project and divide the work because we are going to have a brief presentation for our work in week 5 just after the break.
i have added the headings for the content of our project.
Hey team. i found an article The RNAi revolution from Nature Publishing Group which can be a good sample of our project i think.
Here is the reference
Novina C D, Sharp P A. The RNAi revolution. Nature Publishing Group. 2004. 403:161-164.
for the history/discovery part, should we mention about the unexpected phenomenon observed in transgenic plants experiments?
obiously we should add the research awarded the Nobel Prize in Physiology or Medicine in 2006 "Potent gene silencing effect after injecting double stranded RNA into C. elegans" in the history of RNAi.