Difference between revisions of "Talk:Group 2 Project - RNA Interference"

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--[[User:Z3252340|z3252340]] 07:48, 5 May 2010 (UTC) Your page i really good. I found it was just the right length, not too much text but just enough to get across the basic concepts. I particularly like your history section - it was short and sweet but had just the right amount of information (no one wants to be bored by a huge slab of irrelevant material). Referencing of images could be improved, just on a couple of images though. If they are your own work, copyright them and state from what image they are based upon. A description of what the image is about would also help.
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Revision as of 18:48, 5 May 2010

--z3252340 07:48, 5 May 2010 (UTC) Your page i really good. I found it was just the right length, not too much text but just enough to get across the basic concepts. I particularly like your history section - it was short and sweet but had just the right amount of information (no one wants to be bored by a huge slab of irrelevant material). Referencing of images could be improved, just on a couple of images though. If they are your own work, copyright them and state from what image they are based upon. A description of what the image is about would also help.



Hello I enjoyed reading the webpage. The schematic diagram with the description has enhanced my understanding on the RNAi. You have included a wide coverage of the applications for RNAi. But, just be mindful about the copyright issue when a image from other source is uploaded. Thanks. --z3178608 07:36, 5 May 2010 (UTC)


yes it is working now.

thanks Gabriel. yeh i spent hours for it haha =)

Jae


now that the page is working today..

great job on the pic jae!

--Gabriel Gutnik 00:47, 5 May 2010 (UTC)

oh and latest research shows that rnai is ineffective in malaria work, so i will take out the link for it

http://nar.oxfordjournals.org/cgi/content/abstract/gkp239v1

--Gabriel Gutnik 14:09, 4 May 2010 (UTC)


it's an easy one to draw ourselves, it's all step by step process, so it is a good one to diagram ourselves using a basic model

do it in paint? i can whip it up if you'd like, should be easy --Gabriel Gutnik 14:06, 4 May 2010 (UTC)


hey

as we should put the "diagram drawn by student" within the page, i'd like to put my drawing for the basic mechanism of RNAi instead of the one what i put on before.

have look and please give me a comment.

cheers

Jae


good idea on the glossary, and the page is quite peer orientated anyway

i think we should still stick with the idea of the applications, after all, that is what rnai is used for - i.e. the basis of why it is important.

i'll finish up the applications tonight. there are also some extra touch ups i will take care of - some of the referencing needs to be clarified. --Gabriel Gutnik 02:19, 4 May 2010 (UTC)


Sequence-specific inhibition of small RNA function. Hutvágner G, Simard MJ, Mello CC, Zamore PD. PLoS Biol. 2004 Apr;2(4):E98. Epub 2004 Feb 24. PMID: 15024405

Effective expression of small interfering RNA in human cells. Paul CP, Good PD, Winer I, Engelke DR. Nat Biotechnol. 2002 May;20(5):505-8. PMID: 11981566

Hey i found a really good article

http://www.academicjournals.org/bmbr/PDF/Pdf2006/DEC/Reddy%20et%20al.pdf

Jae


i think we dont need to keep the types of RNAi..

Short interfering RNAs (siRNAs) - siRNA is a short, 20 to 25 base pairs long, double stranded RNA molecule which is involved in the RNAi.

MicroRNAs (miRNAs)

Tiny non-coding RNAs (tncRNAs)

Small modulatory RNA (smRNA)

Jae


hey team. check this site. i think Darren also know this from molecular biology subject. http://www.hhmi.org/biointeractive/rna/rna_interference/01.html

Jae


Oh, hey. sorry i just checked the page now. yeh we all should know the basic consept of RNAi technique. by the way i will be free from 12 to 3 and after the lab.

Jae


OK finally done the page up a bit so we at least have something decent to present tomorrow, most of this information was just from me skimming journals and from my molecular biology notes from last year so this is far from the final product. We should go in much more depth for when the assignment its due. We also need to organise how we're gonna address this thing tomorrow. --Darren Dizon 12:17, 13 April 2010 (UTC)


Hey guys, Sorry for late reply, For tomorrows presentation i think we should all have a basic understanding of RNAi so that we can give a brief report on it. ALso i agree with the choice of headings that Jae has provided us and think we should further discuss which subheadings we will focus on for the assignment, tomorrow. I will upload some summarised points onto the wiki page later tonight

--Darren Dizon 08:35, 13 April 2010 (UTC)

Hi guys.

we should discuss about this project and divide the work because we are going to have a brief presentation for our work in week 5 just after the break.

Jae


i have added the headings for the content of our project.

Jae


Hey team. i found an article The RNAi revolution from Nature Publishing Group which can be a good sample of our project i think.

Here is the reference

Novina C D, Sharp P A. The RNAi revolution. Nature Publishing Group. 2004. 403:161-164.

Jae


for the history/discovery part, should we mention about the unexpected phenomenon observed in transgenic plants experiments?

obiously we should add the research awarded the Nobel Prize in Physiology or Medicine in 2006 "Potent gene silencing effect after injecting double stranded RNA into C. elegans" in the history of RNAi.

Jae