Talk:Group 1 Project - Fluorescent-PCR

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Revision as of 17:35, 5 May 2010 by Z3256270 (talk | contribs)

--Joseph Chuk 07:34, 5 May 2010 (UTC) The comparison and application parts are very good and clear. Images are relevant but seems better putting them all on the right side. The principle and procedures are too detailed that is a bit confusing.

--Samantha Cabrera 07:23, 5 May 2010 (UTC)hjhkjhkhkhui

Hello boys!

Sorry for late reply, I could not get access to this website so doing applications before lab, hopefully will have lots done by then.

See you in lab! --Mari Fushimi 01:02, 5 May 2010 (UTC)

Hey Tom and Mari

Nice work, Tom. But, I will still need two more applications for the PCR to make it three applications in total. Please get most of the assigned tasks completed by tonight. Subsequently, we should improve on the layout and the reference links of the wikipage. Hopefully, we will get a good grading for our page.

Cheers, Clyde --z3178608 22:22, 2 May 2010 (UTC)

Hello Boys!

Clyde awesome set up of timeline and structure, I am in the process of recapping a brief definiton of F-PCR and conventional PCR (C-PCR) to put before the comparisons. Also I am about to read a whole lot of other articles in bed to get my head around it a bit more, its being a while since biochem days! I'm sorry for replying so late guys, nursing assignments have been hectic but I'm going to solidly work on this for the next couple of days! Over and out.


--Mari Fushimi 13:17, 26 April 2010 (UTC)

Hey Tom The magnesium is in the buffer and the buffer could be Tris solution. It is added to facilitate the enzymatic activity of the polymerase therefore acting as a cofactor.

Clyde --z3178608 00:05, 21 April 2010 (UTC)

Hey guys, i've decided to do the timeline for PCR. Also Clyde, is magnesium in the buffer or is it THE buffer? Just wondering, cause you may have the wording wrong. Tom--Thomas Fox 23:25, 20 April 2010 (UTC)

Hey Tom

I think Dr Hill did mention about the deadline during the lab. It is on week 8. And the other groups will be evaluating our wikipage and giving advice for improvement. We have to amend the wikipage accordingly and submit as our final project to be graded.


--z3178608 02:32, 17 April 2010 (UTC)

Sounds great Clyde! I agree that the workload should be managed equally, so it's a team effort, as it should be. btw, when is the project due? Tom--z3187043 02:25, 16 April 2010 (UTC)

Hey Tom and Mari

I was wondering if you are agreeable with the project plan? If everything's fine, we should probably proceed with the contents for each section of the web page. I understand that everyone has their own commitments, so I'm suggesting that we could probably delegate the workload equivalently among us? I'm currently working on the principles of F-PCR in terms of its underlying mechanism. Perhaps, both of you could decide on either the development of PCR or the section on comparison against conventional PCR. For the last section, I think each of us could work on one current application of F-PCR, which makes it three applications in total. Can you let me know your thoughts about this arrangement? I'm truly sorry if I sounded too pressurizing because the deadline is approaching. I am confident that our teamwork would make this project a success with pleasant teamwork experience.

Hope to hear from you soon!

Best regards,Clyde --z3178608 09:57, 15 April 2010 (UTC)

Hey Clyde, the layout and plan for our project looks good, it was along the lines of what i was thinking, which is a good thing. What do you think Bonnie? Don't forget we need to include our student designed figure somewhere, i'm suggesting the fluorescent pcr procedure section, but we should decide as a group. Sorry about the slow reply, bt i was away for most of the break, and recovering from an incident i will tell you about. Also, we could think about splitting up the workload of the project into smaller more easily handled sections ie. the sections below?--z3187043 11:06, 11 April 2010 (UTC)

Hey Tom and Mari

How are you? For this project, I am thinking of including the history, procedures of fluorescent PCR, comparison of conventional PCR with fluorescent PCR, application of F-PCR in molecular biology analysis followed by the conclusion. The procedures would cover the underlying mechanism in the DNA amplification, fluorescence tagging and analysis. The history provides a brief description that begins with the development of conventional PCR, which eventually improvise into F-PCR. In addition, I have applied for article reuse for one of the journal article before we could utilize the material. If there is any suggestions, we could discuss through the discussion board or arrange a meet-up.


Best regards, Clyde

--z3178608 08:56, 6 April 2010 (UTC)