Difference between revisions of "Talk:Cell Mitochondria"

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Powering the Cell: Mitochondria XVIVO Scientific Animation  https://youtu.be/RrS2uROUjK4
 
Powering the Cell: Mitochondria XVIVO Scientific Animation  https://youtu.be/RrS2uROUjK4
  
Mitochondria Fusion Fission Movie
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===Mitochondria Fusion Fission Movie===
  
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File:Mitochondria fusion and fission movie.mp4
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https://vimeo.com/107211731
  
 
Just a simple fluorescence confocal timelapse of a cell labeled with mitoRFP in order to image mitochondrial dynamics, with indicated regions displaying fission and fusion events.
 
Just a simple fluorescence confocal timelapse of a cell labeled with mitoRFP in order to image mitochondrial dynamics, with indicated regions displaying fission and fusion events.
  
  
<html5media height="500" width="500">https://vimeo.com/107211731</html5media>
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Latest revision as of 11:23, 13 April 2017

2017

Powering the Cell: Mitochondria XVIVO Scientific Animation https://youtu.be/RrS2uROUjK4

Mitochondria Fusion Fission Movie

File:Mitochondria fusion and fission movie.mp4

https://vimeo.com/107211731

Just a simple fluorescence confocal timelapse of a cell labeled with mitoRFP in order to image mitochondrial dynamics, with indicated regions displaying fission and fusion events.



What are the Evolutionary Origins of Mitochondria? A Complex Network Approach

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134988


Mitochondria localize to the cleavage furrow in mammalian cytokinesis

PLoS One. 2013 Aug 21;8(8):e72886. doi: 10.1371/journal.pone.0072886. eCollection 2013.

Lawrence EJ1, Mandato CA. Author information Abstract Mitochondria are dynamic organelles with multiple cellular functions, including ATP production, calcium buffering, and lipid biosynthesis. Several studies have shown that mitochondrial positioning is regulated by the cytoskeleton during cell division in several eukaryotic systems. However, the distribution of mitochondria during mammalian cytokinesis and whether the distribution is regulated by the cytoskeleton has not been examined. Using live spinning disk confocal microscopy and quantitative analysis of mitochondrial fluorescence intensity, we demonstrate that mitochondria are recruited to the cleavage furrow during cytokinesis in HeLa cells. After anaphase onset, the mitochondria are recruited towards the site of cleavage furrow formation, where they remain enriched as the furrow ingresses and until cytokinesis completion. Furthermore, we show that recruitment of mitochondria to the furrow occurs in multiple mammalian cells lines as well as in monopolar, bipolar, and multipolar divisions, suggesting that the mechanism of recruitment is conserved and robust. Using inhibitors of cytoskeleton dynamics, we show that the microtubule cytoskeleton, but not actin, is required to transport mitochondria to the cleavage furrow. Thus, mitochondria are specifically recruited to the cleavage furrow in a microtubule-dependent manner during mammalian cytokinesis. Two possible reasons for this could be to localize mitochondrial function to the furrow to facilitate cytokinesis and / or ensure accurate mitochondrial inheritance.

PMID 23991162 PMCID: PMC3749163 DOI: 10.1371/journal.pone.0072886

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072886


Mitochondria-cytoskeleton associations in mammalian cytokinesis

Cell Div. 2016 Mar 18;11:3. doi: 10.1186/s13008-016-0015-4. eCollection 2016.

Lawrence EJ1, Boucher E1, Mandato CA1.

Abstract

BACKGROUND: The role of the cytoskeleton in regulating mitochondrial distribution in dividing mammalian cells is poorly understood. We previously demonstrated that mitochondria are transported to the cleavage furrow during cytokinesis in a microtubule-dependent manner. However, the exact subset of spindle microtubules and molecular machinery involved remains unknown.

METHODS: We employed quantitative imaging techniques and structured illumination microscopy to analyse the spatial and temporal relationship of mitochondria with microtubules and actin of the contractile ring during cytokinesis in HeLa cells.

RESULTS: Superresolution microscopy revealed that mitochondria were associated with astral microtubules of the mitotic spindle in cytokinetic cells. Dominant-negative mutants of KIF5B, the heavy chain of kinesin-1 motor, and of Miro-1 disrupted mitochondrial transport to the furrow. Live imaging revealed that mitochondrial enrichment at the cell equator occurred simultaneously with the appearance of the contractile ring in cytokinesis. Inhibiting RhoA activity and contractile ring assembly with C3 transferase, caused mitochondrial mislocalisation during division.

CONCLUSIONS: Taken together, the data suggest a model in which mitochondria are transported by a microtubule-mediated mechanism involving equatorial astral microtubules, Miro-1, and KIF5B to the nascent actomyosin contractile ring in cytokinesis.

KEYWORDS: Actin; Cytokinesis; Cytoskeleton; KIF5B; Microtubules; Miro; Mitochondria


Myosin XIX (Myo19) as being involved in the regulation of mitochondrial distribution in dividing HeLa cells


PMID 27030796 PMCID: PMC4812650 DOI: 10.1186/s13008-016-0015-4

2014

Int J Cell Biol. 2014;2014:709828. Epub 2014 Jan 22. Formation and Regulation of Mitochondrial Membranes. Schenkel LC, Bakovic M. Author information

Abstract Mitochondrial membrane phospholipids are essential for the mitochondrial architecture, the activity of respiratory proteins, and the transport of proteins into the mitochondria. The accumulation of phospholipids within mitochondria depends on a coordinate synthesis, degradation, and trafficking of phospholipids between the endoplasmic reticulum (ER) and mitochondria as well as intramitochondrial lipid trafficking. Several studies highlight the contribution of dietary fatty acids to the remodeling of phospholipids and mitochondrial membrane homeostasis. Understanding the role of phospholipids in the mitochondrial membrane and their metabolism will shed light on the molecular mechanisms involved in the regulation of mitochondrial function and in the mitochondrial-related diseases.


phosphatidylglycerol (PG) and cardiolipin (CL), are exclusively components of mitochondrial membrane (Figure 1). PC and PE are the most abundant phospholipids, comprising 40 and 30% of total mitochondrial phospholipids, respectively. PA and PS comprise 5% of the total mitochondrial phospholipids [6, 7]. Unlike plasma membrane, mitochondrial membranes contain high levels of cardiolipin (~15% of total phospholipids) and low levels of sphingolipids and cholesterol


PMID 24578708

Biochim Biophys Acta. 2014 Apr;1841(4):595-609. doi: 10.1016/j.bbalip.2013.11.014. Epub 2013 Dec 6. MAM (mitochondria-associated membranes) in mammalian cells: Lipids and beyond. Vance JE. Author information

Abstract One mechanism by which communication between the endoplasmic reticulum (ER) and mitochondria is achieved is by close juxtaposition between these organelles via mitochondria-associated membranes (MAM). The MAM consist of a region of the ER that is enriched in several lipid biosynthetic enzyme activities and becomes reversibly tethered to mitochondria. Specific proteins are localized, sometimes transiently, in the MAM. Several of these proteins have been implicated in tethering the MAM to mitochondria. In mammalian cells, formation of these contact sites between MAM and mitochondria appears to be required for key cellular events including the transport of calcium from the ER to mitochondria, the import of phosphatidylserine into mitochondria from the ER for decarboxylation to phosphatidylethanolamine, the formation of autophagosomes, regulation of the morphology, dynamics and functions of mitochondria, and cell survival. This review focuses on the functions proposed for MAM in mediating these events in mammalian cells. In light of the apparent involvement of MAM in multiple fundamental cellular processes, recent studies indicate that impaired contact between MAM and mitochondria might underlie the pathology of several human neurodegenerative diseases, including Alzheimer's disease. Moreover, MAM has been implicated in modulating glucose homeostasis and insulin resistance, as well as in some viral infections. Copyright © 2013 Elsevier B.V. All rights reserved. KEYWORDS: Apoptosis, Autophagy, Endoplasmic reticulum, Lipid transport, Mitochondria, Neurodegeneration

PMID 24316057

2013

PLoS One. 2013 Oct 18;8(10):e76941. doi: 10.1371/journal.pone.0076941. eCollection 2013. Sigma-1 receptor chaperone at the ER-mitochondrion interface mediates the mitochondrion-ER-nucleus signaling for cellular survival. Mori T1, Hayashi T, Hayashi E, Su TP. Author information

Abstract The membrane of the endoplasmic reticulum (ER) of a cell forms contacts directly with mitochondria whereby the contact is referred to as the mitochondrion-associated ER membrane or the MAM. Here we found that the MAM regulates cellular survival via an MAM-residing ER chaperone the sigma-1 receptor (Sig-1R) in that the Sig-1R chaperones the ER stress sensor IRE1 to facilitate inter-organelle signaling for survival. IRE1 is found in this study to be enriched at the MAM in CHO cells. We found that IRE1 is stabilized at the MAM by Sig-1Rs when cells are under ER stress. Sig-1Rs stabilize IRE1 and thus allow for conformationally correct IRE1 to dimerize into the long-lasting, activated endonuclease. The IRE1 at the MAM also responds to reactive oxygen species derived from mitochondria. Therefore, the ER-mitochondrion interface serves as an important subcellular entity in the regulation of cellular survival by enhancing the stress-responding signaling between mitochondria, ER, and nucleus. PMID 24204710

2011

J Cell Biol. 2011 Jan 10;192(1):7-16. doi: 10.1083/jcb.201006159. Making heads or tails of phospholipids in mitochondria. Osman C1, Voelker DR, Langer T. Author information

Abstract Mitochondria are dynamic organelles whose functional integrity requires a coordinated supply of proteins and phospholipids. Defined functions of specific phospholipids, like the mitochondrial signature lipid cardiolipin, are emerging in diverse processes, ranging from protein biogenesis and energy production to membrane fusion and apoptosis. The accumulation of phospholipids within mitochondria depends on interorganellar lipid transport between the endoplasmic reticulum (ER) and mitochondria as well as intramitochondrial lipid trafficking. The discovery of proteins that regulate mitochondrial membrane lipid composition and of a multiprotein complex tethering ER to mitochondrial membranes has unveiled novel mechanisms of mitochondrial membrane biogenesis. PMID 21220505

Lecture Audio 2013

The University has a system for automated recording of lectures called Lectopia. Lectopia requires login using your student number and unipass. I will be adding the link to each iLecture Audio following the Lecture. Due to the automated recording method, most lectures begin 4-5 minutes into MP3 recordings and occasionally stop before the lecture does.


Archive: 2013 | 2012 | Printable version 2012 Lecture | 2010 | 2009

MH - note that content will not match exactly current lecture structure but has been selected as having similar content


2008 | 2007 Lecture Slides PDF

Movie Powering the Cell - Mitochondria

New insights into the role of mitochondria-associated endoplasmic reticulum membrane

Int Rev Cell Mol Biol. 2011;292:73-117.

Fujimoto M, Hayashi T. Source Cellular Stress Signaling Unit, Cellular Pathobiology Section, Integrative Neuroscience Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, DHHS, Baltimore, Maryland, USA.

Abstract

The mitochondria-associated endoplasmic reticulum membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) membrane that regulates ER-mitochondria communications. The MAM is characterized by direct apposition to a mitochondrion, a unique lipid profile, and the expression of a unique set of proteins involved in Ca(2+) signaling, phospholipid biosynthesis, protein folding, and membrane tethering. The association of the MAM with a mitochondrion is in part cytoskeleton independent and dynamically changed by an elevation of the cytosolic Ca(2+) level. The mechanisms underlying the genesis of MAM are unclear but might involve COPI-dependent vesicular transport and soluble NSF attachment protein receptor. The MAM is recognized as a center for intermembrane transport of phospholipids and for direct Ca(2+) transmission to mitochondria that activates the tricarboxylic acid cycle. However, MAM might be also involved in the interorganelle transport of cholesterol, ceramides, ATP, and proteins as well as in proteasomal protein degradation and lipid droplet formation. Recent studies have begun to unveil the importance of interorganelle communication in the innate immune response to virus infection and in the pathophysiology of neurodegenerative/neurodevelopmental disorders. Thus, drug discovery aimed at regulating ER-to-mitochondria communication may open a new avenue in treatments of human diseases. Copyright © 2011 Elsevier Inc. All rights reserved.

PMID 22078959


2010

Genes Dev. 2013 Dec 15;27(24):2615-27. doi: 10.1101/gad.229724.113. Hallmarks of a new era in mitochondrial biochemistry. Pagliarini DJ1, Rutter J. Author information

Abstract Stemming from the pioneering studies of bioenergetics in the 1950s, 1960s, and 1970s, mitochondria have become ingrained in the collective psyche of scientists as the "powerhouses" of the cell. While this remains a worthy moniker, more recent efforts have revealed that these organelles are home to a vast array of metabolic and signaling processes and possess a proteomic landscape that is both highly varied and largely uncharted. As mitochondrial dysfunction is increasingly being implicated in a spectrum of human diseases, it is imperative that we construct a more complete framework of these organelles by systematically defining the functions of their component parts. Powerful new approaches in biochemistry and systems biology are helping to fill in the gaps. KEYWORDS: biochemistry; mitochondria; proteomics PMID 24352419 PMCID: PMC3877752 DOI: 10.1101/gad.229724.113

Mitochondria and the culture of the Borg: understanding the integration of mitochondrial function within the reticulum, the cell, and the organism

Bioessays. 2010 Nov;32(11):958-66. doi: 10.1002/bies.201000073. Epub 2010 Sep 7.

Braschi E, McBride HM. Source University of Ottawa Heart Institute, Ottawa, Ontario, Canada. Abstract As endosymbionts, the mitochondria are unique among organelles. This review provides insights into mitochondrial behavior and introduces the idea of a unified collective, an interconnected reticulum reminiscent of the Borg, a fictional humanoid species from the Star Trek television series whereby decisions are made within their network (or "hive"), linked to signaling cascades that coordinate the cross-talk between mitochondrial and cellular processes ("subspace domain"). Similarly, mitochondrial dynamics are determined by two distinct processes, namely the local regulation of fission/fusion and the global control of their behavior through cellular signaling pathways. Indeed, decisions within the hive provide each mitochondrial unit with autonomous control of their own degradation, whereby mitochondrial fusion is inactivated and they become substrates for autophagy. Decisions within the subspace domain couple signaling pathways involved in the functional integration of mitochondria with complex cellular transitions, including developmental cues, mitosis, and apoptosis. Copyright © 2010 WILEY Periodicals, Inc.

PMID 20824657


Cytochrome c maintains mitochondrial transmembrane potential and ATP generation after outer mitochondrial membrane permeabilization during the apoptotic process

J Cell Biol. 2001 Apr 16;153(2):319-28.

Waterhouse NJ, Goldstein JC, von Ahsen O, Schuler M, Newmeyer DD, Green DR.

Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA.

Abstract

During apoptosis, cytochrome c is released into the cytosol as the outer membrane of mitochondria becomes permeable, and this acts to trigger caspase activation. The consequences of this release for mitochondrial metabolism are unclear. Using single-cell analysis, we found that when caspase activity is inhibited, mitochondrial outer membrane permeabilization causes a rapid depolarization of mitochondrial transmembrane potential, which recovers to original levels over the next 30-60 min and is then maintained. After outer membrane permeabilization, mitochondria can use cytoplasmic cytochrome c to maintain mitochondrial transmembrane potential and ATP production. Furthermore, both cytochrome c release and apoptosis proceed normally in cells in which mitochondria have been uncoupled. These studies demonstrate that cytochrome c release does not affect the integrity of the mitochondrial inner membrane and that, in the absence of caspase activation, mitochondrial functions can be maintained after the release of cytochrome c.

PMID: 11309413 http://www.ncbi.nlm.nih.gov/pubmed/11309413

J. Cell Biol. Vol. 153 No. 2 p.319

Waterhouse et al.

Video 1 (1.5MB)

Loss and regeneration of {Delta}{Psi}m after cytochrome c release. Cc-GFP-HeLa cells were treated with actinomycin D (1 µM) in the presence of N-benzoylcarbonyl-Val-Ala-Asp-fluorome-thylketone (zVADfmk) (100 µM), and confocal images were taken every 2 min. The cytochrome c-GFP (green, left) shows the coordinate release of cytochrome c in the individual cells (the staining goes from punctate to diffuse upon release). TMRE fluorescence in the same cells (red, right) shows the loss and recovery of {Delta}{Psi}m. The red and green images are of the same cells taken at the same time. The frames are separate rather than overlaid for clarity, and a mathematical representation of loss and regen-eration of {Delta}{Psi}m in a similarly treated cell is shown in Fig. 4 A.