Difference between revisions of "Talk:Cell Biology Methods"

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PC2 Lab page SOMS Wiki
 
PC2 Lab page SOMS Wiki
 
http://somswiki.med.unsw.edu.au/somswiki/index.php?title=PC2_OGTR_Lab
 
http://somswiki.med.unsw.edu.au/somswiki/index.php?title=PC2_OGTR_Lab
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The major disinfectants fall into four groups and their relative merits can be summarised as follows:
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Hypochlorites (e.g., Sodium Hypochlorite)
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Good general purpose disinfectant
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Active against viruses
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Corrosive against metals and therefore should not be used on metal surfaces e.g. centrifuges
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Readily inactivated by organic matter and therefore should be made fresh daily
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Should be used at 1000ppm for general use surface disinfection, 2500ppm in discard waste pots for disinfecting pipettes, and 10,000ppm for tissue culture waste and spillages
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Note: When fumigating a cabinet or room using formaldehyde all the hypochlorites must first be removed as the two chemicals react together to produce carcinogenic products.
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Phenolics
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Phenolic based disinfectants should never be used as they are not supported as part of the EU Biocidal Products Directive review programme.
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Alcohol (e.g. Ethanol, Isopropanol)
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Effective concentrations: 70% for ethanol, 60-70% for isopropanol
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Their mode of activity is by dehydration and fixation
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Effective against bacteria. Ethanol is effective against most viruses but not non-enveloped viruses
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Isopropanol is not effective against viruses
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Aldehydes (e.g. Formaldehyde)
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Aldehydes are irritants and their use should be limited due to problems of sensitisation
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Should only be used in well ventilated areas.
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Formaldehyde is used to fumigate laboratories. The formaldehye is heated in a device so it will vaporise and all exposed surfaces are coated with the disinfectant.
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Generally the use of aldehydes for disinfection and fumigation purposes can be hazardous. Check local regulations and with your safety advisor.

Latest revision as of 10:49, 31 August 2011

PC2 Lab page SOMS Wiki http://somswiki.med.unsw.edu.au/somswiki/index.php?title=PC2_OGTR_Lab

The major disinfectants fall into four groups and their relative merits can be summarised as follows:

Hypochlorites (e.g., Sodium Hypochlorite)

Good general purpose disinfectant Active against viruses Corrosive against metals and therefore should not be used on metal surfaces e.g. centrifuges Readily inactivated by organic matter and therefore should be made fresh daily Should be used at 1000ppm for general use surface disinfection, 2500ppm in discard waste pots for disinfecting pipettes, and 10,000ppm for tissue culture waste and spillages

Note: When fumigating a cabinet or room using formaldehyde all the hypochlorites must first be removed as the two chemicals react together to produce carcinogenic products.

Phenolics Phenolic based disinfectants should never be used as they are not supported as part of the EU Biocidal Products Directive review programme.

Alcohol (e.g. Ethanol, Isopropanol)

Effective concentrations: 70% for ethanol, 60-70% for isopropanol Their mode of activity is by dehydration and fixation Effective against bacteria. Ethanol is effective against most viruses but not non-enveloped viruses Isopropanol is not effective against viruses

Aldehydes (e.g. Formaldehyde)

Aldehydes are irritants and their use should be limited due to problems of sensitisation Should only be used in well ventilated areas.

Formaldehyde is used to fumigate laboratories. The formaldehye is heated in a device so it will vaporise and all exposed surfaces are coated with the disinfectant.

Generally the use of aldehydes for disinfection and fumigation purposes can be hazardous. Check local regulations and with your safety advisor.