Talk:Cell Biology Method - Gene Expression
HEK-293 cells (E1-transformed HEK cells)
- HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture.
- They are also known, more informally, as HEK cells.
- This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
- The transformation resulted in the incorporation of approximately 4.5 kilobases from the viral genome into human chromosome 19 of the HEK cells.
- The line was cultured by scientist Alex Van der Eb, in the early 1970s, at his lab at the University of Leiden, Holland.
- The transformation was executed by Frank Graham, another scientist Van der Eb's lab, who invented the calcium phosphate method for transfecting cells.
- The source of the cells was a healthy, aborted fetus.
- The name HEK293 is thusly named because it was Frank Graham's 293rd experiment.