Talk:Cell Biology Method - Gene Expression

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HEK-293 cells (E1-transformed HEK cells)

  • HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture.
  • They are also known, more informally, as HEK cells.
  • This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
  • The transformation resulted in the incorporation of approximately 4.5 kilobases from the viral genome into human chromosome 19 of the HEK cells.
  • The line was cultured by scientist Alex Van der Eb, in the early 1970s, at his lab at the University of Leiden, Holland.
  • The transformation was executed by Frank Graham, another scientist Van der Eb's lab, who invented the calcium phosphate method for transfecting cells.
  • The source of the cells was a healthy, aborted fetus.
  • The name HEK293 is thusly named because it was Frank Graham's 293rd experiment.