Difference between revisions of "Talk:Cell Biology Method - Gene Expression"

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* The name HEK293 is thusly named because it was Frank Graham's 293rd experiment.
 
* The name HEK293 is thusly named because it was Frank Graham's 293rd experiment.
  
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http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-1573.3&Template=cellBiology
  
 
http://hek293.com/
 
http://hek293.com/

Revision as of 11:52, 31 August 2011

HEK-293 cells (E1-transformed HEK cells)

  • HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture.
  • They are also known, more informally, as HEK cells.
  • This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
  • The transformation resulted in the incorporation of approximately 4.5 kilobases from the viral genome into human chromosome 19 of the HEK cells.
  • The line was cultured by scientist Alex Van der Eb, in the early 1970s, at his lab at the University of Leiden, Holland.
  • The transformation was executed by Frank Graham, another scientist Van der Eb's lab, who invented the calcium phosphate method for transfecting cells.
  • The source of the cells was a healthy, aborted fetus.
  • The name HEK293 is thusly named because it was Frank Graham's 293rd experiment.

http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-1573.3&Template=cellBiology

http://hek293.com/