--Anna Cao 12:47, 21 May 2009 (EST) I felt that the heading "Cathespin L" may be unnecessary and that the content under this heading could be included in the introduction. Also an image would be nice. Good work!
B) overall you've clearly done your reading but perhaps some of the text is oversummarized? paragraphs may have sufficed and more detail could be provided than was in the dot points... research was obviously extensive so it would have been good to see more material... some diagrams also :D feel free to contact me if you'd like further feedback - --Peter Kehoe 12:58, 21 May 2009 (EST)
Hi there, I think the page could be improved with few images to breakdown the text. I like how your paragraphs and sentences are short so it does not look too text heavy. However, i think there are too many horizontal lines within the page... maybe use the next subheading by adding an extra = in the headings so it is still bold and big but without the line...
- Easy to understand language. Under Cathepsin L structure "Cathepsin L utilizes "reverse binding mode" in which enzymatic activity is inhibited by the binding of a substrate in the substrate binding cleft in reverse. this is a general mechanism for utilizing a peptide substrate and at the same time provide immunity to proteolysis." - Probably put a capital for 'This'. Perhaps you can also put more information on Cathepsin L roles. "The action of Cathepsin L in tumor stroma degradation is important due to the acidic microenvironment in the range of it's pH optimum." - I wasn't too sure what this meant. Maybe you can rephrase it? "Cathepsin L expression is induced early during sepsis and correlates with the intensity of protein catabolism in the muscle and is thus a good marker." - you could probably break this down to 2 sentences with the last sentence being "Thus it is a good marker." The final sentence probably shouldn't have 'thus' in it as it was used in the previous sentence. The last sentence also seems to repeat what was said just above it - perhaps you can provide a summary of what you've said about cathepsin L overall instead? Hope that helps but I like the ease that I read your project with. Good luck!
You have now created your individual project page.
--Mark Hill 09:56, 20 April 2009 (EST) You have not yet selected your individual project protein/method topic and still missing some homework items. 2009_Student#Individual_Projects Individual Projects
--Mark Hill 18:33, 19 March 2009 (EST)You still need to provide feedback for Lecture 4 - Nucleus.
--Mark Hill 22:37, 30 March 2009 (EST) Thank you for your feedback on the nucleus, you now have some understanding of nuclear structure and its regulation by the internal skeleton. Now you have to complete the second part, which was to feedback What you found difficult to understand about Lecture 5 Exocytosis. Mmmmnnnn. spaghetti!
--Mark Hill 09:53, 3 April 2009 (EST)Thank your for your feedback. I was really after the type of cellular processes that require lots of energy and so would be sites of mitochondrial location, for example intracellular transport along microtubules.
--Mark Hill 09:54, 3 April 2009 (EST) Correct definitions for CAM terms, lots of science today uses acronyms (because the terms or gene names are very long), but what this can mean is that several acronyms can mean different things to different people.
Lecture 4 - The nucleus.
I found the nuclear lamins really interesting. They are only found in the nucleus and assemble and disassemble during mitosis/meiosis. The lamins are pegged around the nucleus and I think it is interesting how they contribute to the structure of the nucleus. A mutation can lead to the nucleus being folded with an unstable structure - Hutchinson-GUildford Progeria syndrome.  Lecture 5 - Exocytosis.
I find exocytosis process simple to follow. The golgi apparatus however was a bit complex in understanding. The cisternae have no direct communication with each other and each cisternae contains different modification enzymes. The vesicles fuse at different layers of the golgi for modification. Where and for what reason they do this confused me.
Mitochondria are the energy generators for cells. They create energy through a process called cellular respiration or oxidative phosphorylation. They have an important enzyme called ATPase which helps convert oxygen and nutrients into ATP. Apart from generating energy, the mitochondria can also synthesis proteins and replicate DNA. It is also important for storage of calcium ions.  CAM types
N-CAM: Neural Cell Adhesion Molecule
Ng-CAM: Neuron-glia Cell Adhesion Molecule
L-CAM: Liver Cell Adhesion Molecule
I-CAM: Intercellular Cell Adhesion Molecule  Lecture 10
What is the name of the epidermal layer between the basal and granulosa layer and how does it relate to intermediate filaments? The epidermal layer between the basal and granulosa layer is the stratum spinosum. the stratum spinosum has cuboidal cells that are joined together by desmosomes. These cells actively synthesize intermediate filaments called cytokeratins which are anchored to the desmosomes providing structural support to the skin.
Lab 6 - "If you've seen differences in the distribution of phenotypes in Tm4 over-expressing B35 cells versus control B35 cells, describe these differences. Formulate a hypothesis with regards to what changes on the molecular level may have occurred due to the over-expression of Tm4 that lead to morphological changes that you have observed"
Confocal Microscopy - What are the 2 main forms of generating confocal microscopy?
- laser scanning
- spinning disk
Lecture 15 - Cell Cycle - What does "S" stand for in the S phase? The S phase stands for synthesis of DNA. DNA replication occurs in the S phase.