Difference between revisions of "Talk:3186815"

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--[[User:S8600021|Mark Hill]] 07:47, 29 March 2009 (EST) Thank you for your feedback. [[2009_Lecture_4|Lecture 4 - Cell Nucleus]] did not discuss cell adhesion, so I find you comment a little confusing. Have a look at the nucleus information again. [[2009_Lecture_5|Cell Export - Exocytosis]] I am glad you found the content easy to understand and to listen too. Yes, the unique nuclear lamin skeleton will be discussed in [[2009_Lecture_10|Cytoskeleton - Intermediate Filaments]] and [[2009_Lecture_16|Cell Division]].
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== My Reviews ==
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3186582
  
 +
2209471
  
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3191801
  
 +
3217565
  
Tumor Necrosis Factor (TNF)
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3219393
Individual Project - Cell Biology
 
  
OMIM Resource:
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3235019
http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191160
 
  
What is TNF?
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== Peer Reviews ==
Structure
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A. Z3280894 You need to add pictures to your page. Also I suggest reordering your page so that it flows better, like making function below structure. Great job on referencing, you seemed to have done a lot of research. Maybe it would improve your page if you made the cited text link down to the references.
Function: main and specific, esp. in inducing apoptosis.
 
Signaling (inc. 2 receptors for TNF - TNFR1, TNFR2)
 
TNF and its role in Apoptotic Cell Death:
 
Abnormalities:
 
Significance (of research):
 
Future Research:
 
  
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta ( LYMPHOTOXIN), but they share TNF RECEPTORS.
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B. z3217578: An image on your page linking to one or more sections would be helpful for the reader, as well as make your page look nicer. Otherwise, the information is presented well, and clearly explained.
  
http://www.genecards.org/cgi-bin/carddisp.pl?gene=TNF
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C. z3189925: Well written. Would have added some images to the page to better understand the topic and of course add some colour. First impressions are important. As to referencing, although it is not major issue, I would have used the wiki reference list style. This would help readers focus on the content rather than skim past references.  
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis
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factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and
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D. hey, u got content but a bit not helpful to have no pictures to understand what you're trying to say.
thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved
 
in the regulation of a wide spectrum of biological processes including cell proliferation,
 
differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated
 
in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout
 
studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
 
UniProtKB/Swiss-Prot: TNFA_HUMAN, P01375
 
Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by
 
macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing
 
fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the
 
induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce
 
cell differentiation.
 
http://www.ncbi.nlm.nih.gov/pubmed/10634209: Out of the almost 17 members of the TNF superfamily, TNF is probably the most potent inducer of apoptosis. TNF activates both cell-survival and cell-death mechanisms simultaneously. Activation of NF-kB-dependent genes regulates the survival and proliferative effects pf TNF, whereas activation of caspases regulates the apoptotic effects. TNF-induced apoptosis is mediated primarily through the activation of type I receptors, the death domain of which recruits more than a dozen different signaling proteins, which together are considered part of an apoptotic cascade. This cascade does not, however, account for the role of reactive oxygen intermediates, ceramide, phospholipases, and serine proteases which are also implicated in TNF-induced apoptosis. This cascade also does not explain how type II TNF receptors which lack the death domain, induce apoptosis. Nevertheless, this review of apoptosis signaling will be limited to those proteins that makeup the cascade.
 
http://www.ncbi.nlm.nih.gov/pubmed/11431320:Good for mutation stuff – mutations inhibit apoptosis and induce metastasis in breast cancer.
 
TNF – Individual Project for Cell Biology
 
  
Pubmed abstract on TNF-induced apoptosis
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E. Quite informative - images would definitely help though! If you can't find any, maybe draw your own? Especially under the heading "TNF-α Receptors & Signaling". It's good that you linked to a video though. A glossary would also be helpful if people without cell-bio background were to read it...  
http://www.ncbi.nlm.nih.gov/pubmed/10634209
 
  
Mediation of TNF-induced Apoptosis by p38
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F. Probably, the best written project I have reviewed so far. It provides quite a good overview of Tumor Necrosis Factor Alpha and the progression of the scientific knowledge of it. The page may benefit from some pictures, to demonstrate a function of TNF alpha as a multifunctional ligand (draw an example maybe?). Aside from that it was well formatted.
http://www.ncbi.nlm.nih.gov/pubmed/15265709
 
  
Mitochondria in TNF-induced Apoptosis
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G. Appropriate information was given about this particular protein. I have a question: ''known to be one of the most significant members of its class.''  --> do you mean out of all TNF family? What does it mean by significant? It will be  better if you can put pictures on page so that the reader will have some visual information about this protein. Nonetheless, it a well structured page from well background research. (3222840)
Good article/research paper:
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http://www.genebee.msu.su/cmm/skulach.html
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H. You have all the important information here, maybe just a bit more on the current research.  It would also be good to have images of the protein and diagrams to help with the understanding.  (3221652)
 +
 
 +
== HOMEWORK ==
 +
'''LECTURE 4: Outline something new that you learnt from this lecture.'''
 +
 
 +
I learnt about Nuclear Bodies (Cajal and PML) that, to my recollection, I hadn't heard of previously. I also learnt some things about chromosomes that were fairly new. Also, I liked the picture of Nuclear Pores. I had heard about them for a good while but could never really visualise what they might look like - kinda cool.
 +
 
 +
 
 +
'''LECTURE 5: What did you find difficult to understand?'''
 +
 
 +
Everything was pretty understandable because you have a nice voice that's very easy to listen to; like the voices on TV. Perhaps when we study the Cytoskeleton and ECM later on I can build on my knowledge of the structural role of intermediate filaments.
 +
 
 +
 
 +
'''LECTURE 7: What are the main energy processes in the cell?'''
 +
 
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Mitochondria is an organelle in cells. The main function of mitochondria is to produce energy (ATP) for the cell using raw materials such as oxygen and fatty acids. In plant cells chloroplasts are highly involved in energy production.
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 +
 
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'''LECTURE 8: There are different types of Cell Adhesion Molecules present within the Nervous System that each serve a different role'''
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N-CAMs are Neuronal Cell Adhesion Molecules.
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Ng-CAMs are Neuron-glia Cell Adhesion Molecules involved in promoting the regeneration of a nerve subsequent to damage. In addition to this Ng-CAMs are associated with adhesion across the axon.
 +
 
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L-CAMs are Liver Cell Adhesion Molecules that are involved in maintaining the architectural integrity of the liver with cell-cell adhesion.
 +
 
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I-CAMs are Intercellular Adhesion Molecules that are closely associated with the inflammatory response. They often mediate adhesive interactions important for antigen-specific immune responses and are are involved in WBC recirculation when they act to block cell ahesion.
 +
 
 +
 
 +
'''LECTURE 10: What is the layer of the skin that contains a high concentration of intermediate filaments that form desmosomes?'''
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(Hint: between the Statum Granulosum and Stratum Basale layer of the skin).
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This layer is the Stratum Spinosum.
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'''LAB 6: If you've seen differences in the distribution of phenotypes in Tm4 over-expressing B35 cells versus control B35 cells, describe these differences. Formulate a hypothesis with regards to what changes on the molecular level may have occurred due to the over-expression of Tm4 that lead to morphological changes that you have observed'''
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Differences:
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Hypothesis:
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'''LECTURE 14: What are the 2 main forms of generating confocal microscopy?'''
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1 - Laser scanning
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2 - Spinning Disc
 +
 
 +
 
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'''LECTURE 11:What does "S" stand for in the S phase?'''
 +
 
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Synthesis.

Latest revision as of 19:55, 24 May 2009

My Reviews

3186582

2209471

3191801

3217565

3219393

3235019

Peer Reviews

A. Z3280894 You need to add pictures to your page. Also I suggest reordering your page so that it flows better, like making function below structure. Great job on referencing, you seemed to have done a lot of research. Maybe it would improve your page if you made the cited text link down to the references.

B. z3217578: An image on your page linking to one or more sections would be helpful for the reader, as well as make your page look nicer. Otherwise, the information is presented well, and clearly explained.

C. z3189925: Well written. Would have added some images to the page to better understand the topic and of course add some colour. First impressions are important. As to referencing, although it is not major issue, I would have used the wiki reference list style. This would help readers focus on the content rather than skim past references.

D. hey, u got content but a bit not helpful to have no pictures to understand what you're trying to say.

E. Quite informative - images would definitely help though! If you can't find any, maybe draw your own? Especially under the heading "TNF-α Receptors & Signaling". It's good that you linked to a video though. A glossary would also be helpful if people without cell-bio background were to read it...

F. Probably, the best written project I have reviewed so far. It provides quite a good overview of Tumor Necrosis Factor Alpha and the progression of the scientific knowledge of it. The page may benefit from some pictures, to demonstrate a function of TNF alpha as a multifunctional ligand (draw an example maybe?). Aside from that it was well formatted.

G. Appropriate information was given about this particular protein. I have a question: known to be one of the most significant members of its class. --> do you mean out of all TNF family? What does it mean by significant? It will be better if you can put pictures on page so that the reader will have some visual information about this protein. Nonetheless, it a well structured page from well background research. (3222840)

H. You have all the important information here, maybe just a bit more on the current research. It would also be good to have images of the protein and diagrams to help with the understanding. (3221652)

HOMEWORK

LECTURE 4: Outline something new that you learnt from this lecture.

I learnt about Nuclear Bodies (Cajal and PML) that, to my recollection, I hadn't heard of previously. I also learnt some things about chromosomes that were fairly new. Also, I liked the picture of Nuclear Pores. I had heard about them for a good while but could never really visualise what they might look like - kinda cool.


LECTURE 5: What did you find difficult to understand?

Everything was pretty understandable because you have a nice voice that's very easy to listen to; like the voices on TV. Perhaps when we study the Cytoskeleton and ECM later on I can build on my knowledge of the structural role of intermediate filaments.


LECTURE 7: What are the main energy processes in the cell?

Mitochondria is an organelle in cells. The main function of mitochondria is to produce energy (ATP) for the cell using raw materials such as oxygen and fatty acids. In plant cells chloroplasts are highly involved in energy production.


LECTURE 8: There are different types of Cell Adhesion Molecules present within the Nervous System that each serve a different role

N-CAMs are Neuronal Cell Adhesion Molecules.

Ng-CAMs are Neuron-glia Cell Adhesion Molecules involved in promoting the regeneration of a nerve subsequent to damage. In addition to this Ng-CAMs are associated with adhesion across the axon.

L-CAMs are Liver Cell Adhesion Molecules that are involved in maintaining the architectural integrity of the liver with cell-cell adhesion.

I-CAMs are Intercellular Adhesion Molecules that are closely associated with the inflammatory response. They often mediate adhesive interactions important for antigen-specific immune responses and are are involved in WBC recirculation when they act to block cell ahesion.


LECTURE 10: What is the layer of the skin that contains a high concentration of intermediate filaments that form desmosomes?

(Hint: between the Statum Granulosum and Stratum Basale layer of the skin). This layer is the Stratum Spinosum.


LAB 6: If you've seen differences in the distribution of phenotypes in Tm4 over-expressing B35 cells versus control B35 cells, describe these differences. Formulate a hypothesis with regards to what changes on the molecular level may have occurred due to the over-expression of Tm4 that lead to morphological changes that you have observed

Differences:

Hypothesis:


LECTURE 14: What are the 2 main forms of generating confocal microscopy?

1 - Laser scanning

2 - Spinning Disc


LECTURE 11:What does "S" stand for in the S phase?

Synthesis.