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==Apoptosis Inducing Factor (AIF)==

Revision as of 18:49, 20 May 2009

Lecture Feedback

Lecture 4 - Nucleus

Nucleosomes- Formed by DNA wrapped around histones.

Lecture 5- Exocytosis

How does the synthesis from amino- to carboxy- terminal of protein happen in the Ribosome?

Lecture 7- Mitochodria

What types of cellular processes require lots of energy from the mitochondria?

  • Muscle cells (cardiac, skeletal and smooth muscle cels), for contraction.
  • Sperm, within the tail for motility.
  • Hepatocytes, for liver detoxification.

Lecture 8- Cell Junctions

  • L-CAM; liver cell adhesion molecule
  • I-CAM; intercellular cell adhesion molecule
  • Ng-CAM; neuron-glia cell adhesion molecule

Lecture 10- Intermediate Filaments

  • Stratum Spinosum; this web-like system contain intmediate filaments which attach to desmosomes.

Lecture 15- Cell Cycle

  • S- Phase: synthesis phase.

Lab 6: Effect of Tm4 expression on db cAMP induced differentiation of B35 cells

Description of phenotype: B35 cells

Both genotype A and genotype B share similar morpholology. The cells were primarily pronged or stringed.

Genotype A:

  • showed higher concentration of cells all together, and primarily pronged.
  • more neurites in the cells, but shorter and thinner.

Genotype B:

  • showed lower number of cells, but primarily stringed.
  • less neurites within the cells but longer and thinner.

From these results we can suggest that the Tm4 mutant cells compared to the WT (wild type) B35 cells, influence the nutrites of the cells, influencing the morphology of the cells. Thus showing that tryptomyosin specificity differs to suit the cytoskeletons specific role.

Lab 7: Confocal Microscopy

Two methods:

  • Laser.
  • Spinning Disc.


--Beatrix Palmer 15:52, 11 May 2009 (EST) mark, could you please remove an uploaded pic, z crystal structure AIF. thanks.

--Mark Hill 09:22, 16 April 2009 (EST) All on track, kee up the good work!

--Mark Hill 18:13, 19 March 2009 (EST) Thank you for your feedback. Yes nucleosomes are important, not only for DNA packing, but also in our initial understanding of how apoptosis works.

  • Formed by DNA wrapped around histones.

--Mark Hill 15:25, 25 March 2009 (EST) The complete ribosome does not exist unless attached to a messenger RNA, it occurs as the 2 major subunits in the cytoplasm. Initial binding of a subunit occurs at the 5' amino- encoding end of the mRNA and the other subunit now assembles to form the complete ribosome. t-RNA now docks and brings the first amino acid and protein synthesis at AA1 has begun.The next AA is added and synthesis occurs as the ribosome translocates (moves) along the mRNA to the 3' carboxy- terminal at the end of the forming protein.

--Mark Hill 22:44, 31 March 2009 (EST) Yes both these processes, but I had told you these in the lecture, so it was not as if you have tried to find additional cellular processes that require lots of energy. --Beatrix Palmer 15:56, 5 April 2009 (EST) I did look on the internet after class as well, i just thought you wanted a couple of examples. sorry.

  • Muscle cells (cardiac, skeletal and smooth muscle cels), for contraction.
  • Sperm, within the tail for motility.

--Mark Hill 09:39, 3 April 2009 (EST) Correct for CAM terms, lots of science today uses acronyms (because the terms or gene names are very long), but what this means is that several acronyms can mean different things to different people.