Talk:2016 Group 6 Project
- 1 General Organisation
- 2 Lab 3 Assessment
- 3 z5105710
- 3.1 1. T lymphocytes need less than 3 min to discriminate between peptide MHCs with similar TCR-binding parameters, Brodovitch, A., et al. 
- 3.2 2. The direction of migration of T-lymphocytes under flow depends upon which adhesion receptors are engaged, Dominiquez, GA., et al. 
- 3.3 3. Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells, Chang, CW., et al.
- 3.4 4. Restrictions to HIV-1 replication in resting CD4+ T lymphocytes, Pan, X., et al., a review
Factsheet & Roadmap
Drive: https://drive.google.com/folderview?id=0B73R6WMeqvwfRDk5VjBhbW5GLTg&usp=sharing (View Only link, edit link will be provided in private)
Z3465531 (talk) 18:02, 24 March 2016 (AEDT) Hi everyone, I expected to have work on Good Friday, but it turns out that I do not and that I am available. If we need to meet at any time, I am available.
Z3465531 (talk) 15:41, 24 March 2016 (AEDT) Hi Everyone, I apologize again that I will not be able to meet tomorrow to discuss the outline. I am definitely available by phone or skype after 5 pm or perhaps for I could step away for 15 minutes during work if you shoot me a message. I have looked at the feedback for the other projects that were done in 2015, and I think we should be have an outline that looks something like the following:
z3329177 I'm in the main library till 6 i guess, if anyone can meet i would love to join.
Proposals for Structure
4. Add common structure and function
5.b. Mechanisms and development
5.c. Functions and roles in the body
5.d. Clinical implications and diseases
6. Current research, future directions and do as conclusion
In terms of dividing work I think we should do a type of t-cell each, making a glossary and doing references as we go along for our sub type. For the common structure and function and current research we can collaborate at the end Someone can do the intro and someone can do history whilst others can do the smaller subtypes of t cells
1) Banner Heading (as in Group 1 2015)
2) Introduction (as in Group 1 2015, or with timeline graphic as in group 4 with internal referencing, or video as in group 5, careful with citing though) Be sure to cite textbook if used
3) History (Emphasizing research techniques)
4) Types of T Lymphocytes
5) Structure (as in Group 1 2015)
7) Malfunction of T-lymphcyes – investigation of T-Lymphocyte malfunction as a causative agent of disease
8) Current Research – (Possibly switched in order with clinical significance, we’ll see how it feels, good example Group 5 2015 with collapsible tables) -
9) Clinical Significance (as in Group 3 2015)
10) Future Directions (Possibly unify this with the conclusion? Plenty of examples)
11) Conclusion (closing section)
12) Glossary (as in Group 1 2015)
What does everyone think?
4) History (short! keep it on the history of discovery of structure and function, as this is a cell biology class)
3) Common Characteristics (Structure and function of T-Cells compared to other BC's, not too much as specific structure and function of each T-Cell type will be covered in Subsection and they are very different from each other)
Surface Proteins TCR ...
4) Development (Short, not too much immunology)
5) Types (focus on structure and function, Activation, mechanism of action, involved pathways etc.)
6) Clinical Significance (focus on cell biology pathways in the development of the disease)
7) Current research (Involved research centers, current theories related to cell biology of T-Cells etc.) Elaborate this section, he put emphasis on it on a) the lecture b) criticism to former projects
I was looking at the structure, i will add a bit more,but the format could be roughly like this
1. General information (about t-cells, types etc.)
2. History (early and current researches, future expectations)
3. Cell structure
4. Function and mechanisms
5. Related diseases
Division of Topics:
Helper - z3329177
Cytotoxic - z5018866
Memory - z3465531
Suppressor - z5105710
Mucosal associated invariant
Gamma delta T cells -
Suggestions for subtopic
z5105710: T Regulatory Cells: I
z3465531: T Regulatory Cells would be good. Red Blood Cell, Neutrophil, Eosinophil, Basophil, or Monocyte/Macrophage would be great because they always seem to be relevant to our studies. I'l be happy to go with whatever the group decides.
z5018866: I am more than happy to do T regulatory cells, as it would be great to do a topic that someone has a particular interest in. If there isn't enough on t reg cells alone we could maybe do T cells as a whole and z5105710 you could potentially focus on your interest of t reg cells.
z3329177: I'm happy with the topic, because there are many of articles about t-cells that we can access. For the T-Regulatory cells, i think that it is interesting topic, but i will need to learn more about it to be honest.
- Do not cite a review article has having findings. "As reviewed by" is more appropriate terminology. (As in This reference (Biochemistry of tropoelastin. Vrhovski B, Weiss AS. Eur J Biochem. 1998 Nov 15;258(1):1-18. Review. No abstract available. PMID 9851686 ) is a good review article to introduce your topic. One key point is that it is a "review" not original research and should have been cited as such. For example "as reviewed in" or "see review". It is important to discriminate between original research articles and reviews in your writing., group 3 of 2015)
- At all times, remember: How is this sentence related to our topic/subtopic and what are the consequences thereof. Bspw Group 3 2015 made subsection of Vit. A deficiency but didn't explain what this had to do with their exact topic
- At least 1 image on the page should be a student drawn image (e.g flow diagram, power point slide of the cell structure).
- Remember to give all uploaded content an appropriate name.
- Any time that you see nomenclature in an article that is not the current nomenclature (eg. MSDS instead of SDS), that means the article is old and should not be used.
- Avoid add unnecessary detail explaining biological processes that not directly related to the topic (e.g. the description of translation in Group 1 2015)
- It might be a good idea to add "additional information" collapsible boxes (as in Group 3 2015)
Lab 3 Assessment
1. T lymphocytes need less than 3 min to discriminate between peptide MHCs with similar TCR-binding parameters, Brodovitch, A., et al. 
Brodovitch et al. investigated the kinetics of the interaction between MHCs and TCRs. MHC and TCR interaction plays a crucial role in the foundation of "immunological synapses" and discrimination of self and non-self. 1G4 TCR carrying human T-Lymphocytes are dropped on a surface functionalized with various different types, formes (diamonds, squares, crosses) and amounts of pMHCs. To determine the time necessary to differentiate between the distinguished pMHC types, interference reflection microscopy (IRM) was used to quantify the spreading area in real time. The results indicate, that the differentiation process is completed already after 2 minutes, a reflection of how fast our immune system is able to process attacks on our system.
2. The direction of migration of T-lymphocytes under flow depends upon which adhesion receptors are engaged, Dominiquez, GA., et al. 
The movement of leukocytes within vascular environments has given invaluable insight in the progression of auto-immune diseases such as multiple sklerose. The effort of basic research has been promptly rewarded by the discovery of one of the most effective treatments against MS, a monoclonal antibody called Natalizumab, which opts against the delocalisation of lymphocytes from the blood stream into nervous tissue. In the light of such discoveries, further emphasis has been placed on the research of Lymphocyte movement. This paper tried to observe the interaction between lymphocyte function-associated antigen-1 (LFA-1; αLβ2), very late antigen-4 (VLA-4; α4β1) and their ligands, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), cell adhesion molecules expressed on the vascular endothelial lining. VCAM and ICAM was printed on a silicone polymer (PDSM) to inquire, whether ligand type and engagement strenght would influence the migration direction. It was found that direction and cell polarity are well influenced by ligand type and engagement strenght.
3. Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells, Chang, CW., et al.
Induced pluripotent stem cells (iPSC) are one of the biggest promises nowadays. The standard approach, also used in this paper, is viral transfection with Oct3/4, Sox2, c-Myc, and Klf4 . These are the four transcription factors with the power to push cells back to the initial steps of their development. The therapeutic options for iPS are mind-boggling, from Leukemia to Multiple Sclerosis, an uncountable number of diseases is a direct consequence of a malfunctioning immune system. Chang et al. achieved for the first time to induce pluripotent human T-Lymphocytes with expression of various different T Cell Receptors (TCRs). The number of TCRs decreases with the advancing T-Cell development, an increase of incorporation of different TCRs thus signals dedifferentiation. The observed iPSC showed normal patterns of differentiation, as shown in subsequent expression of CD7 and CD3. Furthermore, cytokine signaling strongly resembled natural patterns. The importance of the findings in this paper are emphasized if one takes into account one of the major difficulties with iPSC: directed differentiation. Current approaches focus on mimicking the natural cell environment and copy it from in-vivo to in-vitro, an approach which has been successfully applied by the authors of this paper.
4. Restrictions to HIV-1 replication in resting CD4+ T lymphocytes, Pan, X., et al., a review
Inarguably one of the most important Type of T-Cells are Cells expressing a CD4 surface glycoprotein (not in the Paper, but on a side note: In the range of T-Lymphocytes, CD4 is mainly expressed by T-Helper cells, but also found on other Leukocytes, such as NK-Cells). As reviewed in the paper, besides physiological functions, T-Helper Cells also serve as the main host for HIV1, but only as long as they are in a active state. Active T-Helper Cells primarily reside in lympnodes and replicate not only its own, but also the viral genome. As CD4+ T-Lymphocytes become inactive, a actin structure forms around the cell nucleus and the cell becomes insusceptible for HIV invasion. The review states the cell restriction factor SAMHD1, a deoxynucleoside triphosphate triphosphohydrolase, a key player in inactive CD4+ Cell defense against reverse transcriptase and insertion of viral genom into nuclear DNA.
|Group Projects - Blood Cell Biology - Updated 21 April|
|This year's main topic is Blood Cell Biology. Each group should discuss with group members the specific sub-topic that will be covered by their project.
Here is a list of some of the cell types (Structure and Function)
Embryology - content cannot be reused but a useful resource about cell development.
Histology - images these can be reused in your projects.
|Group Assessment Criteria|
Group Assessment Criteria