Talk:2016 Group 3 Project
|Group Projects - Blood Cell Biology - Updated 21 April|
|This year's main topic is Blood Cell Biology. Each group should discuss with group members the specific sub-topic that will be covered by their project.
Here is a list of some of the cell types (Structure and Function)
Embryology - content cannot be reused but a useful resource about cell development.
Histology - images these can be reused in your projects.
|Group Assessment Criteria|
Group Assessment Criteria
- 1 Assessment
- 2 Discussion
- 3 peer review
- 3.1 Group 3
- 3.2 Group 3
- 3.3 Group 3
- 3.4 Group 3
- 3.5 Group 3 (z3461106)
- 3.6 Group 3 peer review
- 3.7 z5021060
- 3.8 z3463953
- 3.9 z3414546
- 3.10 Group 3 peer review
- 3.11 Group 3
- 3.12 group 3
- 3.13 Group 3
- 3.14 Group 3 Peer Review
- 3.15 Group 3
- 3.16 Group 3 Peer Review (z5020175)
- 3.17 Group 3
- 3.18 Group 3 (z3423497)
- 3.19 Group 3 | B Lymphocytes
- 3.20 z3329177
- 3.21 B Lymphocytes Group 3 - Peer review by z3465531
- 3.22 z5017292
- 4 Lab 3 Assessment
- 4.1 Jing z3461911
- 4.1.1 Role of B cells in the pathogenesis of systemic sclerosis
- 4.1.2 B cell-targeted therapy with anti-CD20 monoclonal antibody in a mouse model of Graves’ hyperthyroidism
- 4.1.3 PAX5 promotes pre-B cell proliferation by regulating the expression of pre-B cell receptor and its downstream signaling
- 4.1.4 Abnormal B-cell cytokine responses a trigger of T-cell-mediated disease in MS
- 4.2 z5021149
- 4.2.1 NF-kB inhibitor blocks B cell development at two checkpoints
- 4.2.2 The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation
- 4.2.3 Effects of lasofoxifene and bazedoxifene on B cell development and function.
- 4.2.4 Zinc transporter SLC39A10/ZIP10 facilitates antiapoptotic signaling during early B-cell development
- 4.3 z5019962
- 4.4 z5016365
- 4.5 z5020356
- 4.1 Jing z3461911
- 5 References
- Z5019962 - 154
- Z3461911 - 93
- Z5021149 - 63
- Z5018925 - 53
- 107 references
- Timeline graph - dates 1890, 1948, 1960 and 1974 are not referenced.
- 1960 Macfarlane Burnet, could have been linked to his reference or Nobel prize.
- Z5019962 - 3 images
- Z3461911 - 3 images
- Z5021149 - 3 images
- Z5018925 - 3 images
- B-cell development and B-cell subsets.jpg
Z3461911 B-cell development and B-cell subsets Deleted for copyright.
- B cell activation.jpeg
Z5021149 T cell dependent/independent B cell activation. Deleted for copyright.
- Th cell–regulated B cell memory development.gif
Z3461911 Th cell–regulated B cell memory development Deleted for copyright.
- B cell functions.jpg
Z5019962 Functions of a B cell. No source cited, Deleted for copyright.
- Z5021149 - :B cell activation.jpeg copyright issue.
- Z3461911 - File:B-cell development and B-cell subsets.jpg|Z3461911 B-cell development and B-cell subsets copyright issue. Copyright © 2016 Elsevier Inc. All rights reserved. The content on this site is intended for health professionals.
- Z3461911 - File:Th cell–regulated B cell memory development.gif copyright issue. Copyright 2015 Annual Reviews. All rights reserved.
- Z3461911 - The three major B cell compartments in peripheral lymphoid organs.jpg no explanation of acronyms
- Z5019962 - B cell functions.jpg No source cited, Deleted for copyright.
Z3461911 (talk) 20:06, 22 March 2016 (AEDT): Hey guys, just wondering which subtopic we should do. If anyone of us also do immuno, the lymphocytes should be right up our alley. What do you guys think?
Z5019962 (talk) Hey guys! Looking forward to working with you all. I don't do immunology but I was actually thinking that lymphocytes would be an interesting topic, or my other thinking was erythrocytes, becauseI think there would be alot of information in that area. I'm not too fussed on the subtopic, so if anyone has strong preferences, we can go with that.
Z5018925 (talk) 11:21, 24 March 2016 (AEDT) http://www.nature.com/nri/journal/v15/n3/full/nri3801.html
Z5018925 (talk) 11:28, 24 March 2016 (AEDT)Looks useful http://www.ncbi.nlm.nih.gov/books/NBK10757/?depth=10
Z5019962 (talk) Hey guys, this review article has some good background info in B cell structure, function and history - might help! http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518873/
- Z5019962 * Pretty sure we're getting peer reviewed tomorrow! Hows everyones sections coming along? * I've started doing the history section aswell as function, but i'm struggling with referencing on the wiki haha * 4/27, 3:55pm * Z3461911 * Ill be working on it tonight * Ill write up the intro * And also start on another suvheading * 4/27, 4:52pm * Z5018925 * Sorry guys have had work * I've done about 1000 words but for a variety of sections * I'm the same as Z5019962 don't understand the referencing * 4/27, 4:53pm * Z3461911 * Sweet * I guess just leave the site where you got the info from * Where the footnote should be * And ill try do it * 4/27, 4:54pm * Z5019962 * Awesome! As long as we all put some info on the page for tomorrow and cover most of the subheadings between us it should be sweet * I know some of the groups are really organised * 4/27, 4:55pm * Z3461911 * It should be fine * 4/27, 5:01pm * Z5019962 * My browser just shut down with most of my work on the wiki not saved😫😫 * 4/27, 5:03pm * Z3461911 * Ah * Thats shit * 4/27, 5:03pm * Z5018925 * God that's frustrating * 4/27, 5:05pm * Z5019962 * So lame, I knew i should have done it in word * 4/27, 6:10pm * Z5021149 * Sorry guys just finished up work. Ya imma do some work on the page tonight and tomorrow before class. * Z5018925 * ahha classic * sorry different subject but Z5019962 do you know if we have to include the questions from the end of the lab in our biochem report? * as in "what is the specific activity? * 4/27, 8:01pm * Z5019962 * Mmm i was wondering the same thing. I think we might! * 4/27, 8:02pm * Z5018925 * Ok thanks ! * April 28 * 4/28, 9:38am * Z5021149 * hey guys, having a but of trouble with the project, how are you guys finding information? * 4/28, 9:38am * Z5018925 * I just used reviews and then went through some of their references * 4/28, 9:39am * Z5021149 * ok, and we can only reference research articles ya? * 4/28, 9:39am * Z5018925 * The reviews have overviews of concepts then direct links to more detailed stuff * Reviews too * As in basic information reviews * Then elaborate with research articles * Does the peer review contribute to the group Mark ? * 4/28, 9:40am * Z5021149 * ok cool thanks. no idea because he hasnt given us a mrks breakdown * 4/28, 9:51am * Z5018925 * 4/28, 10:33am * Z5021149 * sorry I didnt get much up on the page but im moving forward now that Im getting some good info! I spent so much time reading through useless articles * 4/28, 10:34am * Z3461911 * Its alright. Our page looks a lot better compared to last night hahaha * 4/28, 10:35am * Z5021149 * yeah you guys legends * 4/28, 12:06pm * Z5018925 * http://www.bloodjournal.org/content/112/5/1570?sso-checked=true * * B lymphocytes: how they develop and function * T.F.T.: My interest in immune system development was sparked when I applied to graduate school with the specific intention of working with Max Cooper at the University of Alabama in Birmingham (UAB). Joining Max's laboratory in 1980 turned a spark into a passion as I learned of the unique developmen… * bloodjournal.org * May 1 * 5/1, 5:27pm * Z5021149 * I'm working on a computer at uni cause my laptop broke and it just logged me out before saving my work 😭😭😭 * 5/1, 5:41pm * Z5019962 * Oh my god how annoying!!!!! Do it on a word document guys because the same thing happened to me last week 😳 * 5/1, 5:43pm * Z5021149 * Well that's what happened, I had a word doc open and then the whole computer just shut down without saving * 5/1, 5:47pm * Z5019962 * Ohhh right right thats the absolute worst * May 2 * 5/2, 8:45pm * Z5019962 * Is everyone still cool to meet up after the lecture tomorrow? * 5/2, 8:50pm * Z5018925 * Yeah can go till 2:30 * 5/2, 8:56pm * Z5021149 * Yup! Are we meeting to just discuss the project or work on it together? I don't have a laptop yet frown emoticon * 5/2, 9:33pm * Z5018925 * We're just discussing I think? But don't mind what we do. Just need to make sure it's organised into a readable structure * Z3461911 * alrighty. I have a class at 2 though so i cant stay for long * 5/2, 9:53pm * Z5019962 * We need to work out what still needs doing befpre thursday, and we can work on it together for a bit if people have time 😊 Whatever works with everyone * May 3 * 5/3, 1:19pm * Z3461911 *  * 5/3, 1:23pm * Z5019962 *https://embryology.med.unsw.edu.au/embryology/index.php/Immune_System_Development#Spleen_Development * * Spleen Development - Immune System Development - Embryology * The thymus has two origins for the lymphoid thymocytes and the thymic epithelial cells. The thymic epithelium begins as two flask-shape endodermal diverticula that form from the third pharyngeal pouch and extend lateralward and backward into the surrounding mesoderm and neural crest-derived mesenchy… * embryology.med.unsw.edu.au * May 3 * 5/3, 5:51pm * Z5021149 * whoever did the bit in location and activation, is it alright If I get rid of some of it? I'm working on that section atm * 5/3, 6:10pm * Z5018925 * Yeah that's fine I did that part * May 4 * 5/4, 2:50pm * Z3461911 * uhhhh for the person that tried to thumb the embryology picture * you have to upload it first * cause its a different site * 5/4, 2:51pm * Z5019962 * Yep that was me * Okay sweet, thanks. Thought i might have to do that * 5/4, 2:52pm * Z3461911 * yea hahahhaa * liked the ittle note you put in though * 5/4, 2:59pm * Z5019962 * Haha im glad someone saw it * Need to remember to delete it * May 5 * 5/5, 8:06am * Z5018925 * does anyone know how to do a table? * 5/5, 8:07am * Z5019962 * Yep! I did one for history * 5/5, 8:08am * Z5018925 * perfect * 5/5, 8:08am * Z5019962 * So copy the formatting i did for that, and i'll send you a photo of the instructions aswel * 5/5, 8:08am * Z5018925 * ok thanks Z5019962! * 5/5, 8:08am * Z5019962 * 5/5, 8:09am * Z5019962 * No worries! * 5/5, 8:09am * Z5018925 * my god haha that's some serious coding * 5/5, 8:10am * Z5019962 * So all you need to do is the lines and brackets around the text you want in the table. The writing they have there is example text * I know, I'm basically a computer programmer now * 5/5, 8:11am * Z5018925 * ok got you * hahahaa cell biology gets you a double degree * 5/5, 8:21am * Z5018925 * can someone look at the structure one * its gone real weird * i can't figure it out * all good figured it out * 5/5, 8:28am * Z5019962 * sweet * Thursday * 5/12, 12:51pm * Z5021149 * Z5018925, the plan moving forward is for each of us to read the comments about the sections that we wrote and then fix them up. Generaly we need more pictures and more references so keep that in mind! * just confirming the due date with Mark now * 5/12, 12:58pm * Z5021149 * ok so the project is due next week * 5/12, 12:58pm * Z5019962 *http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804284/ * B Lymphocytes: Development, Tolerance, and Their Role in Autoimmunity—Focus on Systemic Lupus... * B lymphocytes are the effectors of humoral immunity, providing defense against pathogens through different functions including antibody production. B cells constitute approximately 15% of peripheral blood leukocytes and arise from hemopoietic stem cells ... * ncbi.nlm.nih.gov * 5/12, 12:58pm * Z5021149 * SO everyone get your edits done on your sections by sunday night, then mon tues wed we can edit eachothers stuff and finish it off * 5/12, 1:00pm * Z5018925 * Jesus only a week! * Ok well it's gonna be a busy weekend then * Sounds good though maybe one day next week we can get together and go through the whole project together * 5/12, 1:03pm * Z3461911 * Yea * Can proof read and make sure everything flows * 5/12, 1:12pm * Z5019962 * Lots to do, but we can get it done * I keep forgetting that there are four of us, so everything that needs doing is divided by four * 5/12, 1:22pm * Z5021149 * Z5018925 is Monday ok to have your stuff done by? Yeah we can totally do it * 5/12, 1:23pm * Z5018925 * Monday I can have it done by, but I can only meet Tuesday for an hour or wed * 5/12, 1:25pm * Z5021149 * Ok cool. Yeah that's all good, we seem to do more work on our own anyways and then just meet up to make sure we are all on the same page * Thursday * 5/12, 4:37pm * Z5018925 * Yeah nice. If we all do our sections in depth there won't be much to fix when we meet. Just little refinements to make it all look good, read well etc * 5/12, 6:49pm * Z5021149 * does anyone know how to cite something twice without having it come up as a seperate reference? * look under development, references 3 and 4 * 5/12, 6:50pm * Z3461911 * Huh i thought they would be the same * 5/12, 6:51pm * Z5021149 * yeah weird, I just assumed it would pick up that its the same thing * 5/12, 7:05pm * Z5019962 * From what I've seen from the other pages it should happen automatically * 5/12, 7:06pm * Z5021149 * ill go have a look and see if I can see how they did it Legit copy and pasted the same code and that happened * 5/12, 7:28pm * Z5021149 * If you have a good research article on b cells, try googlying th authors last name and the words 'b cell' * im finding that most researchers have more than one article out about b cells, getting a lot of good info! * 5/12, 7:32pm * Z5019962 * Oh sweet, good idea thanks z5021149! * If there are any particularly good ones with info on a range of subheadings, would you mind sending the link here? And i'll do the same smile emoticon * 5/12, 7:33pm * Z5021149 * yup will do! * Z5019962 * Does anyone know if 'Innate- like B cells" / ILBs are a separate sub group to the ones we have on the page, or are they just a different name for one of those types * Ignore that, i answered my own question haha * 5/13, 2:56pm * Z5021149 * 👍 * 5/13, 2:57pm * Z5018925 * How long should our sections be? * Also what is the pubmed referencing * I swear I do it wrong every time * 5/13, 2:58pm * Z5021149 *  * But then obvs stick in the PMID from your article * 5/13, 2:59pm * Z5018925 * A lot of the criticism said it was too detailed but doesn't mark want it from proper research papers ? * 5/13, 2:59pm * Z5021149 * And make sure it's the PMID not PMCID * 5/13, 2:59pm * Z5018925 * Ok sweet thanks * 5/13, 2:59pm * Z5021149 * It's hard to say with length, like just as long as it takes to properly cover the content * 5/13, 3:00pm * Z5018925 * Ok sweet I think I had all the info but it wasn't structured well. I'll keep it detailed but try and make it all relevant/understandable * I know the other reviews said it was confusing but mark would prefer detail coz it shows we've done proper analysis of research articles * Friday * 5/13, 6:20pm * Z5021149 * Yeah that's true * Friday * 5/13, 11:02pm * Z5019962 * Should i make a glossary? We got some feedback about the lack of * 5/13, 11:29pm * Z3461911 * If you have time i guess * If not dont worry about it * Saturday * 5/14, 9:15am * Z5018925 * Yeah that would be good * 5/14, 9:20am * Z5019962 * Cool, i'll go through all the sections once we're finished and do it 😊 won't take long * Sunday * 5/15, 10:41am * Z5018925 * We should include research in every section I think if not every paragraph * Coz mark said the focus should be on primary research so each para could have a main point and then support it with a couple papers * 5/15, 10:52am * Z5021149 * Yeah I think that's a good idea * 5/15, 11:41am * Z5019962 * 👍🏼👍🏼👍🏼👍🏼👍🏼 * 5/15, 12:41pm * Z3461911 *
- Z5018925 * Ok I've done the structure gonna finish up disease tonight * But just need a hand with some of the coding haha I make it worse every time I try * 5/15, 4:11pm * Z5019962 * Amazing * What do you need a hand coding? * 5/15, 4:12pm * Z5018925 * Ok so for example if I say this structure plays a role in maturation and development * I want to link to that section in our page * And also the referencing but I should be able to figure that out * Just coz some aren't pubmed references but apart from that I figured out table and dot points numbers etc are easy * 5/15, 4:13pm * Z5019962 * Hmm i'll see if i can figure that out. I know what you mean though * 5/15, 4:13pm * Z5018925 * Thanks Z5019962! * 5/15, 4:14pm * Z5019962 * Do you guys mind the coloured tables? I can change the colours or make them all the same if you think it looks better. I was just experimenting * 5/15, 4:14pm * Z5018925 * Nah the coloured makes it look really good * I think our page looks great * 5/15, 4:14pm * Z5019962 * Obviously content is more important, i was just trying to make it look pretty * 5/15, 4:15pm * Z5018925 * Once we add everything it will be really good * I think we should try get a couple more photos. I've got a couple * 5/15, 4:15pm * Z5019962 * I think so too * 5/15, 4:29pm * Z5021149 * Coloured tables are good, makes it nicer to look at * 5/15, 6:01pm * Z5019962 * http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804284/ * B Lymphocytes: Development, Tolerance, and Their Role in Autoimmunity—Focus on Systemic Lupus... * B lymphocytes are the effectors of humoral immunity, providing defense against pathogens through different functions including antibody production. B cells constitute approximately 15% of peripheral blood leukocytes and arise from hemopoietic stem cells ... * ncbi.nlm.nih.gov * 5/15, 6:01pm * Z5019962 * not sure if I'm blind, but i can't seem to find the PMID for this article * 5/15, 6:13pm * Z5021149 * 24187614 * for some stupid reason they never list the PMID on the PMC site, you gotta go back to the pubmed page that shows just the abstract * 5/15, 6:14pm * Z5019962 * Cool thanks z5021149 * 5/15, 6:14pm * Z5021149 * no prob * Monday * 5/16, 1:51pm * Z5018925 * I've almost done autoimmune diseases is someone doing lymphomas/leukaemias or do you want me to? * Also there's no lecture today right ? * 5/16, 1:59pm * Z5021149 * I did a section on CLL cause it's the most common leukaemia * 5/16, 2:01pm * Z5018925 * Ok sweet * I can add to it if you like I found a good book in the library on b lymphocytes in human disease * Uses research * 5/16, 2:03pm * Z5021149 * Not much to say other than its an accumulation of B cells, should I expand? * 5/16, 2:03pm * Z5018925 * Nah don't think you need to * That's fine * 5/16, 2:04pm * Z5021149 * Ok cool if you find some stuff to add in go for it, I found some other groups went way too far in depth about a disease and didn't really keep it focused on their cell. I was gonna do a whole thing on treatment but at that point it's really not about the B cell anymore * 5/16, 2:04pm * Z5018925 * I'll just add mine on autoimmune and that's disease section done * Yeah exactly * I just talk about how the B cell normal function becomes pathogenic * And then just symptoms * 5/16, 2:05pm * Z3461911 * is there anything left to do? * 5/16, 2:05pm * Z5018925 * But yeah most of it is specific to B cells * 5/16, 2:06pm * Z3461911 * im working on my references atm * 5/16, 2:06pm * Z5018925 * Is there a lecture today * 5/16, 2:06pm * Z3461911 * apparently not * im not sure * 5/16, 2:06pm * Z5021149 * Yeah that sounds perfect. I'm gonna do the drawing tonight, I'll send you guys a pic of it and then scan it at uni tomorrow. Maddi if I run into any problems with scanning, can I give it to you to do at home? * I'll move our discussion over to the actual page wednesday night/Thursday morning once we are all done * 5/16, 2:20pm * Z5019962 * Yeah sure thing z5021149 * No lecture today * The page is looking awesome * Monday * 5/16, 9:35pm * Z5019962 * Z5018925 you did structure right? And needed help with referencing? * 5/16, 9:36pm * Z5018925 * Yep I've done structure and autoimmune diseases * Just need to get the referencing done but that won't take me long it's all on the word doc * And I'm gonna add 3 pictures tomorrow * 5/16, 9:38pm * Z5019962 * Sweet, i just started changing the PMIDs you had there to references because I wasn't sure if you said you needed a hand with the referencing * 5/16, 10:01pm * Z5018925 * Ok thanks Z5019962. Yeah that all helps but don't worry too much I'll finish it all off soon * 5/16, 10:08pm * Z5019962 * All good. Sorry, I should have asked before doing anything * 5/16, 11:46pm * Z3461911 * i finished referencing my parts * should i add diagrams of what the antibody isotypes look like * like the whole pentamer and dimer structures * Tuesday * 5/17, 7:14am * Z5018925 * Yeah that might be good. * Wait do you mean when they form cross linking networks? Coz I found a good diagram for that * 5/17, 8:00am * Z3461911 * Oh we can use that * I just mean the basic structure * 5/17, 10:14am * Z5021149 * I'm working on the drawing for it! I'm colouring it right now lol * I've got an IgG and IgM drawing * 5/17, 10:43am * Z5021149 * 5/17, 10:44am * Z5021149 * How's that? Just needs labelling. I could do it buuuut my writing looks like a 5 year olds. Does someone want to do it super quick after the lecture? Or should I just do it and not worry about it * 5/17, 10:45am * Z3461911 * Looks good * 5/17, 10:45am * Z5018925 * Yeah that's great * 5/17, 10:45am * Z3461911 * I guess we can scan it and type the labels up * 5/17, 10:45am * Z5018925 * We can label it if you bring it today * 5/17, 10:45am * Z5021149 * Good idea! * 5/17, 10:45am * Z5018925 * Or yeah just type it * Wait are we meeting today ? * Z5021149 * Idk are we? I'll be at uni either way * 5/17, 10:49am * Z3461911 * Sam * Same * 5/17, 10:51am * Z5018925 * Yeah I think we should straight after lecture * Just for a bit * 5/17, 10:51am * Z5019962 * Yeah i think so * Thanks z5021149, looks very nice * 5/17, 10:52am * Z5021149 * Sounds good! * 5/17, 1:32pm * Z5019962 * I did a section on b cell regulation in collagen induced arthritis only to realise afterwards that its the animal version of rheumatoid arthritis * So z5021149 is it okay if i link it in at the end of your rheumatoid section? its relevant * 5/17, 1:40pm * Z5018925 * Hey Z5019962 I did the rheumatoid section * Yeah that would be good * I already mention collagen induced arthritis and the role of regulatory B cells but if you can add to it that would be good * 5/17, 1:44pm * Z5019962 * Oh sorry. Okay sweet i'll do that now. If you want to change any of it because of what you think is relevant thats totally fine smile emoticon * 5/17, 1:47pm * Z5018925 * Ok sounds good I'll read over it tonight when I'm finishing the reference * 5/17, 2:57pm * Z5021149 * How does that look? * 5/17, 2:58pm * Z3461911 * That looks fine * Is that taken with just your phone hahaha? * 5/17, 2:59pm * Z5019962 * Beautiful * 5/17, 2:59pm * Z5021149 * yup lol I know its a bit dark but the scanned one was brighter but the colour didnt really show up * 5/17, 3:00pm * Z5018925 * Nah that looks great * What about the other one you drew ? * 5/17, 3:00pm * Z3461911 * Yea cant be too picky * It shows the important info * 5/17, 3:01pm * Z5021149 * havnt done the other one yet, just wanted to see if that one was ok. Is there anything else that needs t go on it? * 5/17, 3:01pm * Z5018925 * Sweet sounds good * 5/17, 3:01pm * Z5021149 * for the IgM one I wont label all the constant/variable regions * 5/17, 3:01pm * Z3461911 * Yea thats a given * 5/17, 3:02pm * Z5019962 * Yeah if the images are next to each other on the page theres no need * Z5018925 * What section are we including it in? We should refer to it in that section * 5/17, 3:04pm * Z3461911 * Structure of antibodies probably * 5/17, 3:04pm * Z5018925 * Ok I did that part I'll change it a little to refer to the diagram * * 5/17, 3:07pm * Z3461911 * Lemme know if you need references * Ill find some * 5/17, 3:08pm * Z5018925 * Wait what you mean? * 5/17, 3:09pm * Z5021149 * ya do I need to reference the picture? * 5/17, 3:09pm * Z3461911 * I mean for the text * 5/17, 3:09pm * Z5018925 * Oh yeah * We've got enough references for everything else right ? * 5/17, 3:19pm * Z5021149 * I think so! * 5/17, 3:25pm * Z5019962 * How do you reference a non pubmed article? * 5/17, 3:30pm * Z3461911 * Probably insert the reference code between ref></ref? * 5/17, 3:51pm * Z5021149 * 5/17, 4:40pm * Z5019962 * 5/17, 7:19pm * Z5021149 * are we all gonna send the lady our stem cell article or do you guys want to send me the links and ill do it in one email? just found mine * 5/17, 8:17pm * Z5019962 * Someones on top of it! I think it'd be easier if we sent them all together 😊 i can try find one tonight! * 5/17, 8:19pm * Z5021149 * Ok sounds good. I work tomorrow so wanted to find mine tonight lol I can send the lady an email from work but so just post an article by tomorrow afternoonish * 5/17, 8:25pm * Z5019962 * Okay no worries! Thanks z5021149 * We have to send it by Friday right? * Z5018925 * I'll find one tonight and send it * I think it was by tomorrow? I remember 18th for some reason * 5/17, 8:26pm * Z5021149 * no gotta email her by tomorrow 5pm * and then I think she will respond by friday with which article she wants us to do * 5/17, 8:27pm * Z5018925 * Yeah * 5/17, 8:28pm * Z5019962 * Oops haha clearly i was paying attention * 5/17, 8:42pm * Z5021149 * lol * 5/17, 9:00pm * Z5018925 * Did you figure out how to reference non pubmed? * Did Z3461911's code work? * 5/17, 9:16pm * Z3461911 * how many articles do we find each again? * 5/17, 9:17pm * Z3461911 *http://www.nature.com.wwwproxy0.library.unsw.edu.au/nature/journal/v521/n7550/full/nature14415.html * Database Access - UNSW Library * login.wwwproxy0.library.unsw.edu.au * 5/17, 9:18pm * Z3461911 * i guess i have one * Sequential cancer mutations in cultured human intestinal stem cells * that the title if you cant access the link * 5/17, 9:19pm * Z5021149 * Perfect, just one each * 5/17, 9:19pm * Z5019962 * Nah i think it would work though if your article has a reference code. I only needed it for one line in the history section so i found a different reference * 5/17, 9:20pm * Z5018925 * ok sweet * just another question with one photo i'm trying to find the copyright * 5/17, 9:20pm * Z5018925 * it says copyright rockefeller press but then i went to their copyright statement and its bloody long but basically it says : "The Authors acknowledge that RUP will make the Article freely available to the public on RUP’s website after expiration of the Initial Publication Period, and that RUP intends to submit the Article to PubMed Central in accordance with PubMed Central’s requirements, where the Article will be released to the public after expiration of the Initial Publication Period. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share" * 5/17, 9:21pm * Z5018925 * is that all good to use? it has great pictures * 5/17, 9:22pm * Z3461911 * the publication period is over right? * if it is it should be fine * 5/17, 9:22pm * Z5018925 * what is a publication period? * 5/17, 9:23pm * Z5019962 * It's usually 6 months * So if it was published more than 6 months ago you can use it * 5/17, 9:24pm * Z3461911 * oh thats what i means hahahah * 5/17, 9:25pm * Z5018925 * yeah nice that works then * also we need to include a copyright statement of our own accompanying the hand drawn image * 5/17, 9:26pm * Z5021149 * Did other groups do that? * 5/17, 9:29p * Z3461911 * how do you write up a copyright statement? * do you just say that its your file? * 5/17, 9:29pm * Z5018925 * There's an example on his editing basics: image thing * We literally just copy it and add our names * 5/17, 9:46pm * Z5018925 * I've gotta go to bed i've got work real early but I might need a hand with the images tomorrow * I just need to upload one more but I can't get it to have a caption * 5/17, 9:47pm * Z5019962 * Yeah no worries! Let us know/ send it through and we can get it done * 5/17, 10:11pm * Z5019962 * http://www.ncbi.nlm.nih.gov/pubmed/?term=A+mechanism+for+the+segregation+of+age+in+mammalian+neural+stem+cells * Heres my article * 5/17, 10:15pm * Z5018925 * http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854709/ that's mine * Effective combination of human bone marrow mesenchymal stem cells and minocycline in... * Multiple sclerosis (MS) is the most common inflammatory demyelinating disorder of the central nervous system (CNS). Minocycline ameliorates the clinical severity of MS and exhibits antiinflammatory, neuroprotective activities, and good tolerance for long-term ... * ncbi.nlm.nih.gov * Today * 6:25pm * Z5021149 * the lady chose Z5019962s article! tomrrow in the lab we will get some info on how to actual do the project and then I guess we can get started * 6:38pm * Z5019962 * Fabulous. Thanks for sending them though z5021149!
1. Nice diagram for the main wiki page , however introduction could be improved by being more paragraph or essay-like rather than summing up few sentences into 2 paragraphs. 2. Most of the contents are not cited. 3. Headings and sub-headings are well organised, with the use of table in "History" and "Summary of B-cell surface molecules". 4. Student's own drawn diagram with own explanation could be included for better understanding. 5. Interesting extra significant findings about B-cell surface structure are included, making the wiki page more interesting. 6. Relevant information of Red Blood Cells to the study aims of cell biology, showing much information has been collected. 7. More interesting learning diagrams can be included
Introduction ad history: This provided a good overview on what B-cells are and what they do. The history section was concise and gave the key points, however would there be more in text referencing?
Development: just a few possible formatting changes such as the first paragraph to have ‘B cell can differentiate along 2 distinct pathways:
1. Differentiate to form extrafollicular plasmablasts that are essential for rapid antibody production and early protective immune responses.
2. Activated B cells can enter germinal centres, where they can differentiate into plasma cells or memory B cells. Some of these B cells remain in the follicles and become membrane Ig-expressing germinal center cells. This could make it easier to flow through when reading. There are a few minor spelling mistakes and the second paragraph could be slightly re-worded so, again, it is easier to flow through whilst reading.
Location and activation: There are a few grammatical errors that can be fixed and also some slight re-wording. Addition of references in the T cell dependent activation section should be provided. Also probably providing an image or flow diagram how T cell activation and B cell activation occur, from this it will aid in the understanding process and also compare and contrast between the two. Within the T cell dependent activation section, it would also be good to elaborate on immunoglobulin class switching, just to give an idea of what it actually is.
Types of B cell: This section had good referencing. The use of a diagram would be helpful in this section with an explanation of each type of B cell - mainly for regulatory B cells whilst speaking about receptors involved.
Structure: The overview of surface structure section seemed too wordy and could have been broken up into separate sub sections just to make it easier to read through. References should also be added throughout this section.
Function: The overall readability of this section was good. The antibody isotypes could be placed in a table to make it easier to read through. In the first paragraph however, it could be focused more on antibody production rather than elaborate on the experiment. You could possibly explain the main idea of it and reference the article for the viewer’s interest.
Role in disease: This section is well worded, just needs to be completed with the last 2 areas and add in references.
Applications: could add in a current research done in that area
Overall: Information provided was great, just required a few formatting changes, the addition of references and slight re-wording in a few sections to make the flow of the page better, overall great work!
B-Cell Group 3
1. key points clear
- Development: Why do you start the development section with how they get activated? Structure chronologically, I would even suggest putting that in function in a subsection activation
- "microenvironment encourages hematopoietic stem cells to differentiate into progenitor B (pro-b) cells " Why? If you mention it, explain it. if you can't, don't mention it.
- "H and L chain genes must be rearranged to yield a new, unique IgM molecule." Why abbreviation? at least refer to them as heavy and light chains once
- "gene rearrangement occurs". How? Why?
- "Signalling form this new Pre-BCR stimulates the cell to proliferate as a large pre-b cell. After 3-5 rounds of division, these cells are stimulated through BCR signalling to differentiate into small non-dividing pre-b cells (Zhang, 2004)." Not clear, what signaling makes them big, why big, and what signaling makes them small and why small again?
- "In the transition stage of development, B cells undergo various checkpoints to test for auto-reactivity" How?
- "Transitional cells that are found to be auto reactive are either deleted, undergo receptor editing or become anergic" How? what's anergic?
- This is mostly found in organisms that lack t cells". That's incorrect. it doesn't depend on the species but on the antigen. highly repetitive antigens, such as Lipopolysaccharides of gram negative bacteria. Humans have specific B cells that are particularly involved in T independent activation, called B1 and Marginal zone B cells. (source: janeways immunobiology, 2012, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193734/). Also you should include BAF (B Cell activating factors) released by makrophages.
- Types of B Cells: B1 cells have a lot more additional features. They produce primarily IgM and only rarely switch to IgG etc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193734/
- B2 cells: key point about B2 is that they are activated by thymus dependent (TD) antigens in contrast to B1, you have to mention those
- Really like reading the structure section, in contrast to other section it has some meat. but here aswell, you need at least 30 references for such a huge paragraph.
- "As is discussed later, these receptors play a critical role in B cell maturation and development": development and maturation is before, and no, they are not discussed.
- "A study by Robert et al. (1978) utilized immunoprecipitation techniques to detect Lyb-3 (a 68 000 d polypeptide) in the membrane of a specific subpopulations of B-cells. The results demonstrated that Lyb-3 is distinct from IgD and IgM surface components. This suggested that Lyb-3 is involved in the triggering of B-lymphocytes by antigen, and indicate that Lyb-3 may act as a receptor for a T-cell dependent signal." what is lyb-3? what do you want to say with that, why is it there?
- "Another study by Vogel & Haustein (1989) investigated possible mechanisms and candidate proteins involved in transmitting the signal from Ig-crosslinking with the antigen or anti-Ig antibody to eventually elevate expression of MHC Class II molecules within B-cells. Utilising radiolabelling procedures, the researchers tested how the Ig proteins (from spleen cells) interact with the plasma membrane. The study discovered proteins that are disulphide-linked to IgM, one of which traverses the plasma membrane and therefore might be involved in transmitting the signal to the interior of B-cells." same here, don't understand what this means or why it is there.
2. choice of content, headings and graphs.
- insert histology picture of B-Cell
- insert picture of BCR structure and Antibody structure
- Talk more about memory cells, difference between memory cell and plasma cell structre and morphology etc.
- Insert picture of secondary lymphoid organ in Location and Activation
- T-cell Dependent Activation: what molecules are involved etc.?
- You have to add more content, very lean at the moment.
- Really iked the internal structure of Plasma cells, and that the central dogma form follows function is apparent in ER and Golgi etc.
- Generally, way more pictures guys.
- Really lacking referencing (Location and Activation) and the few there is, is wrong (not wiki-style but paper-style)
- B_cell_functions image is not referenced
4. own innovative diagrams, tables or figures and/or using interesting examples or explanations.
- Not there
5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
- Various in depth basic research is include
6. Relates content to cell biology.
- Signaling is not really present, why don't you add singaling e.g. in "Class Switching", this subsection is anyway really thin.
- Please write the B of B cell big. I read 20 times t and b cells which is just not professional
- "How cell surface markers vary in different stages of B-cell differentiation": random bullet points in the text
you have to put in work. Loads of pictures and content is missing, and you have to completely overthink your referencing. there's loads to do and this is way below the average level of the pages
Introduction-second paragraph probably not necessary, too much detail for an introducing
History-good concise history
Development-need the date on the in text reference for the first paragraph. No reference for your diagram. Check wording and grammar. Put the two points in the first paragraph in a list rather than continuous in the paragraph. Ordering of the paragraphs doesn’t seem right-the first one should come last
Location and Activation-good, use of diagram would help
Types-why does a plasmablast become a plasma cell? What causes that differentiation?
Structure-could definitely do with a diagram. Seems to be no referencing at all for this section-where did your information come from? Surface structure is quite unclear and seems to jump around with no flow, consider re-wording. I see the surface markers section seems to have supposed to of being in dot point form-fix the formatting. In the plasma cells section-what do ‘these processes’ refer to? Copy the referencing style from the other significant findings section for the rest of the project. Maybe put in a diagram of an antibody?
Function-try reducing antibody isotypes section down to a simpler table.
Role in disease-what results from autoimmunity? How is it treated? Section is incomplete for the moment.
Applications-could definitely be fleshed out-how are they useful specifically?
Overall-Inconsistent and non existent referencing. Only use in text referencing if making a direct reference to a paper ie. As found in “blah” or “blah” found that. Otherwise just use footnote referencing so the full reference can easily be referred to at the bottom of the page. When referring to the different cell types eg. B-cells or T-cells make sure how you type it consistent eg. capitalization (B or b) and B cell or B-cell
1. Very small history section, perhaps add a few more years in it
2. A simple and concise set out to their report which made it easy and clear to read
3. Lack of glossary and small reference list need to be added and improved upon
4. Good use of images overall
5. Few spelling errors, which the report needs to be looked over before submitting
Group 3 (z3461106)
Information is in great depth. Great command in language and flow of text, especially when explaining the role of calcium recruitment in T-cell independent activation. This allowed me to link my understanding to how B cells induce differentiation without the dependent need of T-cell activation. It is also presented in a concise manner, especially in the Differentiated B cells section in which it provides information of their origins and important functions after B cells are differentiated from hematopoietic stem cells. That is, that they act as APCs and present the antigens to CD4+ T cells. The tabulation of the B-cell surface molecules was a spectacular idea as it nicely summarised which those that exist on the surface and provides a brief explanation of their respective functions.
However, other information such as the Antibody Isotypes, whilst also equally concisely explained, can be tabulated so that it is aesthetically pleasing to the reader and easy to distinguish, comparing them by their prominence in the stage of immune response (i.e. primary or secondary) and their respective half-lives. In addition, more sources need to be cited throughout the whole wiki page, particularly in the Introduction, History, Structure, Function and Role in Disease sections. Furthermore, despite the history of the B cells being short and succinct, extra detail can still be added to provide a profound and broader timeline into the discoveries of the B-cell, which is representative of the amount of information depicted in your wiki. Take advantage of paragraphing. For example, when describing the two distinct pathways of B Cell differentiation. Lastly, utilising more diagrams, especially structure of B cells and their activation would be advantageous, so please consider this during your edits. e.g. When B cells are organised into follicles and exposure leads to germinal centres. Overall, Group 3 has made an excellent effort in the explanation of B cells, however, need to take greater emphasis in using headings, diagrams and citing sources.
Group 3 peer review
Introduction: Good overview, just left asking what in the body do they elicit an immune response to? Is it something specific or just any pathogen?
History: Nice and short, give key points at a glance.
Development: the 1st and 3rd paragraphs are good, the second is a little hard to read and understand. Also the order of the paragraphs seems odd, it would make more sense to have the 1st one last so it is chronological.
Location and activation: In first paragraph second sentence there is repetition. A picture showing the zones of the lymph node would be a great addition here, and would help to make clear of the content. The second paragraph I feel should be reworded introducing the interaction with B-cells as I was a little confused as to why it jumped to macrophages. In the t-cell dependent activation section state that class switching is mentioned as this is something I immediately questioned and started scrolling the page for. The T-cell independent activation is good in content however, could be a little more concise.
Types: What causes the plasmablast to die or differentiate into a plasma cell? The section of regulatory B cells could be expanded on and how they work.
Structure: I found most of the section difficult to follow, breaking it up into just structural features and the function would be good keeping how they do it in another section. “How cell surface markers vary in different stages of differentiation” was particularly hard to read, I feel like it was meant to be in dot points but not formatted correctly. The inclusion of some diagrams of these receptors would help too. The structure of antibodies section is much easier to follow again however, again a diagram would be good.
Function: I feel this should be more towards the top of the page as it is a section I want to know about straight away. Also some of the first paragraph is repetitive. I found it a little confusing talking about the function in terms of an experiment, I feel it would be best just to take the most accepted recent findings and tell me about the function not the experiment. Could the antibody isotypes be put into a table, it would be much easier to compare that way. It also may be useful to add how the receptors carry out their function on the cell, in terms of activation and binding.
Role in disease: The autoimmunity section seems more fitting to the function section. Rheumatoid arthritis section gives a good overview of disease and B cell roles. I assume this section is not finished and more will be added.
Applications: Good section, potentially add current and future works into this section.
Overall you’re on track, major thing is references there seems to be a lot of unreferenced material and some not in the wiki format. The addition of diagrams and pictures is needed throughout. Other than that, just minor grammatical corrections and rewording of some paragraphs needed. Also the banner is really big with B lymphocytes written really small this could be made more proportional. Great work though!
This group also had a good structure. Starting from the introduction, which gives a good overview on lymphocyte B-cells, going on to structure, function and then it's role in disease. The information written by this group is easy to read and simple but in depth enough for a third year medical science student. Although the information was found and written was good, this group also wrote in large paragraphs which tends to cause students to lose interest. Having a more visual approach could be a good option. Pairing the information with more images and utilising bullet points and table to summarise instead of lengthy paragraphs could possibly make the project seem more interesting. Another problem I noticed was this this group did not include any in-text citations at all! They did however have the references at the end. It is important to add an in-text citation with every statement to indicate which information came from where.
A lot of good quality information but a very bland page. Here are some notes: - History is not very extensive - B-cell development and B cell subset image is very small! I would make it twice as large (800px). And maybe describe it in text more - Section: development, 3rd paragraph: spelling error - Instead of using in text referencing, you should use footnotes (easier for the reader to find the references). Remember it doesn’t have to be a pubmed affiliated article to be made a footnote, just put in “” - Section: development. You introduce the term L chains with no description, the reader may have no idea what that is. Make sure you describe newly introduced terms! - There is an underwhelming amount of images used. Images should be used for: o B cell activation o Mind map of different B cells o Different antibodies - Tables are bland as well, try adding some colour! - Evidently you must finish the immunodeficiency/ and b cell cancer sections! - Also go into more depth about the biotechnology applications of B cells and don’t forget references!
- Order of the topics is well thought out, each topic flows well with the next
- History is too short, perhaps include some more recent discoveries
- A lot of detail for development but great explanation
- Location and activation: needs visuals of the lymph nodes, to show where B cells reside, could include a short video for B cell activation and interaction with T cells
- Type of b cells: well written and easy to follow
- Structure: too much information, dot points are better especially for the overview
- Function: offers a good, simple explanation however section on antibody isotypes could be placed into a table for aesthetic purposes. An image of a labelled antibody would also be appropriate in this section as well as diagram depicting how they are produced
- Disease: what is there is good, could offer a couple more examples of specific diseases
- Referencing: I noticed large paragraphs where references weren’t provided and the reference list was quite short considering all the information ono the page.
- Applications- can include more recent developments/current research avenues
- It would be good to include a glossary, some terms I had never come across e.g. anergic, opsonisation etc.
Group 3 peer review
The page is very detailed and provides all the information you would want, however the references are just not there. There are only 18 references for your entire page which is clearly not enough. Some parts do have in-text citations but these should be coded as reference tags, and most sections don't have references at all. The history section is sparse and needs to be added to, and the page needs more images as a whole to accompany the huge blocks of text and make the information easier to digest. The images that you have uploaded, while useful, need to have their information properly filled out (i.e description, reference and copyright), and I believe that the image on B cell development is copyrighted without a creative commons license meaning you can't use it. A self drawn or modified image is also required, and the banner should be made smaller. A couple of the disease sections need to be finished, and a few spelling/grammar issues need to be sorted (proof read the page).
This was a super interesting assignment and I think it was really well written, providing a lot of in depth information about a range of aspects of B-cells.
- The first thing I noticed was the references, especially in the development section. To begin with, I thought that you hadn't referenced, but then I realised some just didn't appear as links. I think its really important that you stick to one type of referencing, especially so you supply a link or a full title for all the sources you've referenced and also provide a sense of continuity, as if only one person had written the page.
- I wasn't sure if the history section has been finished or not because it ends in the 1970's. If there is no more information to be added to this section, it could be an idea to ass a current or future research section.
- The structure section I think provides an awesome opportunity to add some really cool images and diagrams to break up the information. Maybe you could add some images for the surface structure or the antibody structure sections to enhance the really great and thorough information that you have provided.
- The section about the role in disease was really interesting but needs to be finished. The inclusion of the last two diseases that are already headings would add a lot of depth to the section.
- Similarly to structure, I think that the function section could use some images, for example to demonstrate the antibody isotypes and enhance that awesome information.
You guys have done an amazing job and the page provides so much great information that really just needs a few images to boost it and re-iterate some of the concepts. Awesome job!
1. Good overall key points throughout, very thorough
2. I feel like history could be longer with the section only including up to 1976. I feel like more could be added about the history of B cells. On a minor point maybe make the font in the header a little larger to make the B-cells stick out a little more.
3. Citing is an issue with none of the images being references properly, for the first image “all rights reserved” does not give you permission to recreate an image. The second image (EM) includes the pmid but on that page it states the information is copyrighted. The third image gives no reference or copyright information. The development section has no references other than in-text Harvard referencing but with no bibliography I cannot see the full reference.
4. More images would be good on the page with some original images to fill it up a little more.
5. It seems a little unfinished with only 18 references there is more research to be done into the topic
6. It does begin to provide a learning tool however I think more work needs to be done
This page provides sufficient information regarding B cells, with its respective sections providing a solid amount of detail to educate those reading the page. However, the history section requires a lot more detail, and more information regarding events that occurred in more recent years as well. Further, the vast majority of the page does not seem to be referenced in the required manner, and thus the reference list at the end of the page has 18 links only. While there are in text citations throughout the page, I am assuming that they need to be properly referenced so that they get added to the reference list. The actual information is detailed and thorough, with the subsections helping to make it flow and appear organised and informative. The function and the types of B cell sections in particular are very detailed, and extremely educational. The images also provide extra supplementary information to the text, however, I believe that the images need to be edited so that there is a title and small summary (on the actual page) of what the pictures are displaying. The page could also be improved through the addition of more images to break up the long paragraphs of text, especially in areas such as the different B cell types, as well as the function section. Further, some images don’t have the copyright information, or a description of the image when it is clicked on. The applications section is quite unique and a good way to end the page, providing information on the diagnostic uses of the cell type. However, once again, this section is not referenced either, and no in text citations are provided. Additionally, the immunodeficiency section is not completed yet.
On the whole, this group’s page provides well detailed information regarding this cell type, and allows readers to gain a sound understanding of the details of B cells. The accompanying images further enhance the content, and allow for a deeper understand of the topic. However, some sections need to be worked on and completed, and the references need to be added so that the enter page is properly cited and referenced. The addition of more images could also positively benefit this page.
Group 3 Peer Review
The topics are well structured, however more details to the main information present will significantly improve the page. One of the most glaring problems of this page as a wiki page is the referencing style.
Similar to group 2, reference style should just follow the method provided in the course, ie numbered with bibliography list at the end of the page rather than using in-text referencing. More references should also be included in some sections such as introduction, Structure section and the Function section, just to show where the information were obtained from.
Despite this, most of the main information provided in the page are pretty interesting and engaging. History can definitely be expanded further. More images and diagrams related to the processes and histological images (or electron microscopy) should also be added to further engage readers and improve readability. The locations of the images should be fixed as well, for example, the B cell functions image should be put near the top of the section and not going in-between through two different sections.
Glossary can also be added to define terms contained in the main text which are hard to understand but also to avoid unnecessary or unrelated definitions / descriptions to be present in the main sections
Finally, the final two sections, "Role in Disease" and "Application" should be given more examples rather than just one or two.
You guys did a great job on your page. Lots and lots of information on your page but my biggest critique is that there is more text than visual aids and I felt a little overwhelmed when I first scrolled down your page before I started to read. In saying that though the information is easy to read and you guys described the structure very well even though you have no visuals. Additionally having the development before the structure allowed me to understand the text further down the page, which I found helpful. So my biggest critique would be that there needs to be more visuals incorporated only because you don’t want to scare your readers you want to entice them to read and adding visuals helps do that as it balances out the text on your page. Mainly look at functions and types of B cells. Further I noticed that your references don’t flow as in the introduction you have put them in manually while other sections you guys used numbers (wiki referencing). Just be sure to have one style of referencing and not different types, this can benefit with the flow of your project page and also makes it look more presentable.
Group 3 Peer Review (z5020175)
- Clear & written very well - suitable for student learning
- States general info about B cells - where they form? What they do?
- No referencing
- Antibody secreting effector cell is called what? - Plasma cells
- This section could be improved by adding more information and references
- Does not mention any significant individuals.
- Subheadings for the different B cell stages should be included
- Logically outlines the different stages of B cell development but hasn't formatted it well
- Genetic recombination & specificity has not been explored very well
- How are autoreactive B cells deleted or become anergic?
Location and activation:
- Location clearly stated
- Easy to read
- T cell dependent activation is missing information - Types of receptor binding, cytokines released & what class switching occurs?
- T cells and not t cells!
- Writing has great clarity but detail is not enough for student learning
Types of B cells:
- This is an appropriate subheading
- Layout was executed very well
- States what the specific cell type does and where it's located. For example, Memory B Cells are dormant until re-exposure to antigen where they differentiate into plasma cells to secrete antibodies for antigen clearing. Found in germinal centre
- Lacking diagrams
- Lacks referencing
- Appropriate subheadings
- This section was Well written
- Shows complexity in surface marker expression
- Split the dotpoints under surface marker section
- Plasma cell structure explained well and has a good accompanying diagram
- No copyright caption included in diagram
- Summary table of surface molecules is helpful
- Diagram for structure of antibodies
- Summarised well
- Clarity in expression
- Needs more referencing
- What do the cytokines do to maintain immune system? Provide examples!
- Why is class switching such as to IgE or IgA important and where does it occur?
Role in disease:
- Lacks referencing and could be improved by more information
- You should provide a prominent example of an autoimmune disease that involves autoreactive B cells
- The process of forming autoreactive B cells is somewhat explained but lacking key information - Genetic susceptibility, epigenetic factors, environmental factors, loss of immune tolerance
- What about multiple myeloma?
- How does one gene mutation lead to the alteration in B cell signalling? - give an example
- Immunodeficiency? Agammaglobulemia
- What are other applications?
- The written expression is very clear and suited to student level
- However, there is a lack of detail and information - lots of knowledge gaps
- Lack of referencing and images are not from copyrighted source
- The choices of headings and subheadings was good
- No student diagrams & barely any tables
- Introduction flows well and so is development but the latter needs to have subheadings
- History is lacking
- B cell structure and types of B cells is missing diagrams
- Maybe you can have a collage of the different B cell types or a timeline image showing immature B cell to mature one
- The importance of certain functions such as class switching and maintenance of IS was missing
- Role in disease and applications is lacking sufficient information
The introduction was succinct and nicely written. The history section was lacking references and appeared to be quite short. But as I did not research B cells or have much historical background, the amount of information could be a sufficient amount. Under the headings of ‘Development’ and ‘Location and Activation’ the information was well written with references given but the actual links to the paper appeared to be missing. The image in the development section could be made a little bigger. Also, the information under ‘B-cell development and B-cell subsets’ could be pasted on to the main page rather than the image’s own page. Information under ‘Location and Activation’ could easily be understood however again, references appeared to be missing. In some parts of the text lower case was used e.g. ‘b cell’ and ‘t cell’ whereas upper case letters were used for ‘B cell’ and ‘T cell’. Keep all upper case so that wording remains constant and standardised. Referencing approved under the section of ‘Types of B-Cells’. A diagram illustrating B cell differentiation could be added to this section. Additionally, a diagram (maybe even an original illustration) could be added under structure. Good use of table under the heading ‘Summary of B-cell surface molecules’ along with the electron microscope image. The addition of information regarding other significant findings about B-cell surface structure proved to be interesting showing further research was performed. A diagram of an antibody could also be added to aid visual presentation. Thorough information was given about the function of B cells. A good start to information regarding the role of B cells in disease was given, the last 2 diseases mentioned just need to be finished. Information under ‘Applications’ was brief. Possibly try elaborating on one or two of the mentioned applications. Overall, the readability of this project was commendable. However, major additions need to be made on references and possibly an original student diagram.
Group 3 (z3423497)
- Great image used for the title banner but lacks the proper referencing and copyright citation.
- Introduction is detailed but lacks flow. This is present throughout the assignment but can be easily corrected with rearrangement of information.
- History is a bit short and can be expanded on, there is a lack of referencing.
- Choice of topics and subtopics is good but some are incomplete, topics such as role in disease and application can be expanded on.
- More uses of images for the subtopics can help with giving a better understanding of the information and help break down the blocks of text
- Page lacks a student created image but that can be easily added in, possibly an image could be made for the timeline.
Overall the page has most of the information there, it just needs to be rearranged to create a better flow, additional images would help greatly and references need to be added.
Group 3 | B Lymphocytes
History : I feel you could have added more into here! What about when B cells were actually discovered themselves? Isolated? Structure studied? There are surely more notable dates of importance that should be here!
Development : Really helpful and clear diagram used here! Some spelling errors here. A bit hard to work out what H and L chains are from here, especially if this is the first thing the reader is going to be engaging with without having first read the structure section! I wonder if the order of your subsections could potentially be changed, especially since there are structural elements talked about here which haven't been introduced to the reader yet!
Location and Activation : Some grammatical errors. Extensive antigenic sampling of lymphatic fluid? What does this mean, I don't think this is something that would be clearly understood by your readers without your explanation. You talk alot about receptors and the things that bind to them to allow activation of B cells, I wonder if a diagram would be a helpful way of communicating this information to your audience. This section also seems really low on references with whole sections lacking a single reference.
Types of B Cells : A helpful and clear look at the different types of B cells, there life spans and function! You had really clear and concise information here but since you're talking about different types of B cells, perhaps differentiating pictures of them would contrast them well and show some of the structural or functional differences between them.
Structure : My first impression is that there is a huge block of text without references and without clear diagrams supporting the information you're portraying about the structure. A diagram would aid your information about the antigen binding portion of the B cell receptor complex! Your section about cell surface markers is really hard to read and really difficult to understand, you probably need to reword this or at least present it more clearly for the reader to be engaged and learn effectively. Great table about the different cell surface molecules, helps to break up the text and keep the reader up to scratch with what they all do. The other significant findings about the B cell structure was interesting because it connects some of the potential effects and results of having these particular structural traits. In general REALLY low on references.
Function : Solid information about the function, particularly in the different antibody isotopes that have been discovered. This sort of information would be great in a table! Perhaps with columns of : Abundance | Production | Function. Generally solid section!
Role in Disease : No references. Could be filled in more, seems that the writer ran out of time to insert detail here.
Applications : No references. No examples of the actual applications? Eg. therapeutic treatments for cancer, auto immune disease and inflammation? Have there been prior uses before? Reference that or at least give an example of it! Definitely can be flushed out more.
the history section is very well summarised that reader can easily understand. the figure of the development section is very well explained, but it is missing the reference.
I would suggest that contents have to be referenced such as the figure for the banner is very nice but there is missing of the copyright and the student image paragraph. For the order of the subheading should structure and function put first, then the readers can get the idea of the B-cells. the section of the diseases and application should have contained the references, and also there is the figure in the section of the diseases missing of the reference figure of the diseases. creating the glossary part should help the readers understanding. the subheading is confusing, the reader could distract by the subheadings.
B Lymphocytes Group 3 - Peer review by z3465531
1. Are the key points of the topic delineated and clearly presented in detail?
• The key points of the topic were identified as aspects of B cells according to the title figure, structured subheadings, and a clear introduction.
• Clear language was often used.
• As a result, the article was typically easy to follow and informative.
2. Are the content, headings and sub-headings, diagrams, tables, graphs appropriate and do they indicate sufficient comprehension of the allocated topic?
• The content presented was appropriate, but some sections, as detailed below, were either overly specific or not detailed enough.
• The figures were appropriate, but sparse, and do not appear be have an appropriate reference given each’s details. Some sections as mentioned below could benefit from additional figures.
• The title banner could be student authored if the group is looking for a good figure to edit to make a great display of the topic and fulfil the associated requirement.
• A glossary could be a helpful addition to the wiki page.
• An important note that applies to all sections: There are very few citations throughout the wiki. Large sections of the wiki are have no reference attributed at all. This really needs to be corrected. Citations need to be added. Even if a piece of information seems basic, try to find support for it in a review article. Additionally, a common error in other projects, if using a review article, be sure to cite review articles “as reviewed in [#]”.
• Introduction: The introduction is very clear and straightforward and delivers a very clear description of B-cells. The introduction touches on all of the aspects of structure, function, synthesis, previewing the sections to follow without becoming overly detailed. Be sure to add citations, as there is a lot of specific information included here, and source of the information, at the very least a review article, needs to be credited. Otherwise, this is a brilliant introduction.
• History: Timeline is very short and contains only a single citation. Have nothing of any consequence concerning B cells happened since 1976? Look at the History section presented in Group 2 as a good example for referencing in a timeline and a suitable length. Those events described so far, however, do seem significant.
• Development & Location and Activation: These sections are very technical and difficult to follow well. Cells is misspelt at “cels” in one instance and B cells is not consistently labeled with an upper case B. The citations should be made according to the instructions that were given in lab. The figure is very instructive for B-cell development, but the mechanisms in it appear to be highly technical and illegible at the level to which it has been shrunk. If you are unsure how to do this, be sure to review the relevant section in the introductory labs. There are chunks of text dispersed throughout the text that are missing citations. In particular, the author of this section might want to consider creating subsections to assist the reader in understanding the different possible fates in B cell development. Figures would be very valuable here.
• Types of B-cells: While this section still requires more citations, this section models best the practice of developing useful subheadings for the purpose of organizing the information for better instruction to the reader. This section is very clear and provides a great scaffolding for the reader to organize the dense information and better understand the wiki as a whole. Figures would be very valuable here.
• Structure: The section of structure gives a very detailed technical account of the structure of B cells. While the detail at times appears to be excessive and presented in a difficult way for the reader to understand, the summary and figure are useful tools for learning. Be aware that the figure here does not appear to be appropriately cited displaying the license permitting its reproduction. More citations, however, are necessary. This section is completely devoid of citations, which is not acceptable, as the information presented is very clearly from one or more sources in detail. The table of B-cell surface molecules is really valuable, but needs to be cited. It is informative that this section refers back to a concept in function describing the structure justification behind an earlier mentioned functional activity between B cells and T cells.
• Function: This section is worded well and does a good job of dividing up the concepts of B cell function and giving adequate descriptions without becoming too technical. There are some instances in which the author uses citation appropriately, as in the subsection Initiation of T-cell Immune Response. It would be helpful if elsewhere the citations were likewise more frequent and closely associated with the specific information that the specific reference contributed. The content here is some of the densest so far in the wiki, so it would be helpful for some cited pictures and the student authored images to assist the reader’s comprehension. The picture provided is excellent and should be presented earlier in the section, but again, the picture needs to have the license permissions provided, as they currently are not. It looks like the whole team needs to be really careful about this. In general, the section has helpful divisions, but requires more citation.
• Role in Disease: The subsection for autoimmunity here is incredibly clear and well written. It needs to have citations, as it currently has none. Pay attention in many instances to the importance of citing a review article differently than primary research. The citations are integrated into the text well. The RA section is also well written. The sections still needs to be completed, so be sure to do that in a timely manner, and make sure that citations are included as the information is posted.
• Applications: This is a very short section again missing citations, though it gives a succinct account of monoclonal antibody production. A section titled Current Research ought to represent more of the current applications of B cells. The section also seems like an oddly specific for a section serving as the conclusion of the wiki. A separate brief conclusion and glossary might be beneficial.
3. Are citation and references for the topic appropriate?
• There appeared to be a very sparse number of citations where the material presented was not appropriately attributed to the source of the information. Throughout, either information cited from review articles needed to be identified as such or additional citation of information in general was required.
• All four images appeared to have citation major citation errors immediately apparent in the wiki document. One figure, B-cell development and B-cell subsets, actually contained a copyright notice, but the copyright notice does not appear to expressly state that the figure is allowed to be reproduced. It would be a wise course of action to double check for any errors, as these mistakes would be a serious source of concern.
4. Is the wiki instructive to peers by making use of insightful diagrams, tables or figures and/or examples or explanations authored by the group members? • The wiki did at times present material in a manner friendly and instructive to peers, but as mentioned above, the project would benefit from more figures and student designed learning tools to help organize all of the information.
• There was no student created image.
• The figures seem to have generally appropriate descriptive names.
• The Functions of a B cell gives a beautiful picture of the cells being described and their functions. Be sure to find out if you are actually allowed to use it.
• It could be useful to look at some of the media created by students in Group 1 including their banner, clearly depicting megakaryocytes, and summary picture clearly depicting the different biological roles of megakaryocytes.
5. Is it clear that dedicated research has been conducted to connect basic and applied sciences, and does the effort go beyond the formal class material?
• It seems that some research and connections were made between basic and applied sciences, in particular in the sections Role in Disease and Applications, but the content needs to be expanded and requires careful research with the inclusion of citations.
• The students displayed effort to go beyond the formal class material to research their topic in depth, however, as mentioned earlier, much of the information used came from review articles not cited as such, or not cited at all.
6. Does the group relate the content of the wiki to the primary learning aims of cell biology?
The primary learning aims of cell biology are to understand the relationship of structure and function within the cell as well as broadly within the tissue and organism.
• The project addresses structure and function within the sections so titled. The section Synthesis and Regulation and the other sections, particularly Diseases and Abnormalities, help to put B cells in the context of the rest of the body.
7. Final thoughts
Reading this wiki page on B cells was informative and helpful toward advancing understanding of them. The structure and delivery of the information appears to be to the appropriate level and even, at times, perhaps more technical than might be expected of this course. The main areas of focus should be the aforementioned many citation issues and completing some sections such as the History, Development, Role in Disease, Applications, and Conclusion that appear unfinished to varying degrees. In truth, however, all of the sections require work concerning missing citations, especially the figures, which demand immediate attention. Apart from these issues, however, this appears to be a helpful and informative project concerning B cells. Good work, and keep going strong with finalising your project in the coming weeks.
- The banner looks really good although I’m not sure you included all the copyright information when uploading it.
- The introduction needs to include references in it, there are not citations at all.
- The history section is missing references and is incomplete.
- The development section looks great and is references but it would be good to add the numbered references so there is a link to them at the bottom of the page in the reference section.
- Under the location and Activation section there needs to be references.
- The types of B cells section looks good, its nice and concise.
- Again there are no references in the structure section. I like the summary table of the B cell surface molecules but it also needs to be referenced, as does the picture to the right of it.
- A video could be added, maybe in the function section to help explain what they do.
- the immunodeficiency section needs to be filled in, but seems like an interesting section to have.
- I like that the ‘other significant findings’ section is included. It makes the page interesting and is well written.
- I like the subheadings chosen and how the page has been structured, however a lot of sections are missing references completely, or aren’t referenced with a number and link to where it is in the reference list at the bottom of the page.
- The reference list needs to be more extensive which will look better when all the in text citations are added.
Lab 3 Assessment
Role of B cells in the pathogenesis of systemic sclerosis
In this article it is speculated that B cells play a significant role in the pathogenesis in systemic sclerosis. B cells are therefore observed in both within damaged organs and at a systemic level to determine their role in the inflammatory and fibrotic phases during systemic sclerosis. Through this observation, the B cells subset, marginal zone B cells, are found to be harmful through their synthesis of pro-inflammatory cytokines such as TGF beta and IL-4. These cytokines are also growth promoting cytokines which in turn lead to the development of systemic sclerosis.
B cell-targeted therapy with anti-CD20 monoclonal antibody in a mouse model of Graves’ hyperthyroidism
Graves’ disease is characterised by an overproduction of thyroid hormones and thyroid enlargement, which is a result of B-cell- and T-cell-mediated inflammation. This particular study (Ueki et al., 2011) administered anti-mouse CD20 monoclonal antibodies in mouse models of Graves’ disease, which eliminated B cells from the periphery and spleen. In effect, this suppressed serum immunoglobulin levels, splenocyte secretion IFN-γ, and development of hyperthyroidism. B cell depletion was found to be highly effective in preventing disease development.
PAX5 promotes pre-B cell proliferation by regulating the expression of pre-B cell receptor and its downstream signaling
This article examines the Pax5 gene and its role in the development in B cells. It is well known that Pax5 contributes to B cell development but the impact of Pax5 on pre-B cells is unknown. To identify this relationship, the Pax5gene was knocked out of mice and the levels of marker proteins present at various stages of B cell development were monitored. Most marker proteins were observed to be lower than if the Pax5 gene was still present. Due to the lowered amounts, a signalling cascade could not form and therefore the B cells were not able to develop from the pre-B cells.
Abnormal B-cell cytokine responses a trigger of T-cell-mediated disease in MS
The depletion of B cells can have immune consequences are hypothesised to have influence on multiple sclerosis. This article explores this hypothesis by comparing B cell effector-cytokine responses in mulitsclerosis patients. These experiments were conducted both in vivo and ex vivo. Results showed low amounts of proinflammatory responses for cytotoxic and helper T cells. The remaining B cells then made up for these low amounts of T cells. From these results, the article states that lowered B cells mediate " bystander activation" of T cells which result in the relapse of multiple sclerosis.
NF-kB inhibitor blocks B cell development at two checkpoints
During development, B cells start in bone marrow and then move through different stages until they finally mature in the spleen. Various checkpoints, transcription factors and regulatory factors are utilized during this process to ensure only healthy b cells continue down the path of development. NF-kB is an example of a protein complex transcription factor that plays a role in b cell development. In order to identify when NF-kB was acting during development, Feng et al introduced an NF-kB inhibitor (IkBα) via a viral vector. What they found was that in subjects that received the viral IkBα, the reconstitution levels of b cells was much less in bone marrow and spleen compared to the subjects who did not receive the inhibitor. This lead to the conclusion that NF-kB plays a role in the development od B cells in the bone marrow, as well as maturation and survival in the spleen
The Zizimin2 gene has previously been identified to be related to immuno-senescence and filopodia formation in immune tissues. Expression of the gene has been observed in aging mice. There has been little research into how the Zizimin2 gene actually effects the immune response and how the decline in expression of the gene leads to immuno-senescence. Matsuda et al generated Ziz2 knock out (KO) mice to examine what effects removing this gene would have on the immune response. when thy compared the KO mice to wild type, they found that the KO mice had a higher percentage of early bone marrow B cells, but a reduced number of mature marginal zone B cells. The Ziz2 gene clearly has en effect on marginal zone B cell maturation, but had little effect on the folical B cells. Marginal zone B cells play a role in fighting infectious disease by reducing the time that it takes the body to mount a response against the infectious substance. A decrease in the expression of Ziz2 with age may effect the hosts ability to fight off infectious disease by decreasing the fraction of marginal zone B cells.
Effects of lasofoxifene and bazedoxifene on B cell development and function.
Initially, estrogen was seen as an effective treatment for menopausal women with osteoporosis. Estrogen does increase the bone mineral density in women with osteoporosis, however estrogen treatment has been linked with increased risk of endometrial and breast cancer. SERMs are a class of estrogen receptor agonist/antagonists, which act differently depending on the tissue type. Second generation SERMs such as raloxifene have been observed to increase BMD but not increase the weight of the uterus in mice. Estrogen has effects not only on BMD and uterine weight, but also on the immune system. Bernardi et al set out to investigate what effects third generation SERMs such as lasofoxifene (las) and bazedoxifene (bza) have on the immune system compared to traditional estrogen treatments. It was observed that while estrogen treatment lead to a decrease in B cell numbers across all of the developmental stages, while las and bza only effected b cells in the late stages of bone marrow b cell development and splenic transitional 1 b cells.Estrogen treated subjects also had an increase in antibody producing cells while those treated with las or bza did not.
Zinc transporter SLC39A10/ZIP10 facilitates antiapoptotic signaling during early B-cell development
As B cells move through the different stages of development, various requirements must be met in order to for the cell to move down the path of differentiation. Zinc has long been know to be related to immune function as people with Zinc deficiency present with low immune function. While the link has been clearly established, the mechanism behind the lack in immune function has not been demonstrated. Miyai et all set out to find a mechanism behind zinc deficiency by examining the role of the ZIP10 zinc transporter. By down regulating the ZIP10 transporter, decreased levels of Zn were seen in the cell. This activated caspase activity and lead to the cell undergoing apotosis. Overall, the down regulation of ZIP10 lead to a decrease in the population of B cells. This ZIP10-mediated Zn homeostasis is vital for the survival of early B cells which go on to become functional immune cells.
Bruno, Tullia et al. "Antigen Presentation By Tumor Infiltrating B Cells Influences CD4 T Cell Phenotype And Function In Primary Lung Cancer Patient Tumors". Journal for ImmunoTherapy of Cancer 3.Suppl 2 (2015): P397.
This study looks at the role of B cells in non- small cell tumours, and how the correlation of improved survival with increased B cell levels provides some understanding of the function of B cells. It was shown that B cells within the tumour (TIL- Bs) present tumour autologous antigens, and polarized other cells to produce anti- tumour cells, such as T- helper cells.
Shimabukuro-Vornhagen, Alexander et al. "The Immunosuppressive Factors IL-10, TGF-Β, And VEGF Do Not Affect The Antigen-Presenting Function Of CD40-Activated B Cells". J Exp Clin Cancer Res 31.1 (2012): 47.
B cells play an important role in immune stimulation, through their association with antigen- presentation. This article explores whether immunosuppression works against the immunotherapeutic role of B cells, or whether B cells retain their immunostimulatory function.
Shimabukuro-Vornhagen, Alexander et al. "The Immunosuppressive Factors IL-10, TGF-Β, And VEGF Do Not Affect The Antigen-Presenting Function Of CD40-Activated B Cells". J Exp Clin Cancer Res 31.1 (2012): 47.
Teleost fish have a large mucosal surface, via which pathogens can enter the body. As a result of this, it is essential that these organisms possess a mucosal immune system that maintains the balance between pathogenic and non- pathogenic bacteria at these vulnerable points – B cell predominate in this defense system. This study looks at the important role of B cells in gut- immunity and the acquired immune response. It was found that B cells carry out their effect on the gut immune system through their capacity to differentiate into antibody secreting cells, producing immunoglobulins to target pathogens, and presenting antigens to T cells for destruction.
Barsotti, Nathalia Silveira et al. "IL-10-Producing Regulatory B Cells Are Decreased In Patients With Common Variable Immunodeficiency". PLOS ONE 11.3 (2016): e0151761.
This article looks at the link between the absence of regulatory B cells and immunodeficiency, providing valuable insight into the importance of the function of these B cells in immunity. It was found that individuals with common variable immunodeficiency (CVID), generally presented with depressed levels of regulatory B cells, which results in reduced control of T cell activation and autoimmunity.
Introduction has no references. History has only 1 reference and could do with a bit more detail. Referencing is inconsistent – some footnotes and some in-text APA style. These in-text APA style references have not been included in the reference listing. Quite a few spelling errors. Surface structure and other sections would benefit a lot from more diagrams to help the reader’s understanding. The entire page is very sparsely referenced and there are entire sections that are devoid of referencing.
Information: Information was presented well, in a logical sequence. Scientific jargon was defined throughout to enable understanding of the finer details. Depth of information: There was good depth of research sufficient to gain good understanding of main concepts. The “Role in Disease” section needs refinement but is headed towards the right direction in terms of relevance and detail. Conciseness and Readability: Overall, paragraphs had an organised flow of information and direction which enhanced the readability of long sections of text. The use of bullets in the “Types of B-cells” section enhanced the readability of text. Very good paragraphing to highlight new points and progression of information. The lack of spelling and grammatical errors made text easy to read and professional. Layout and Images: Layout was neat and organised with good use of headings and expansion tables. There was good use of images to support corresponding text. Images were labelled well and were relevant to the topics presented. There was also correct use of citation and referencing of images. Referencing: There are many large paragraphs of text which lack references that need to be included. Other than that, referencing was generally consistent and orderly.