Talk:2015 Group 3 Project

From CellBiology
Revision as of 16:11, 4 May 2015 by Z3374116 (talk | contribs)

2015 Projects: Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7

--Mark Hill (talk) 08:42, 21 May 2015 (EST) Your Group Project will now have peer feedback from the class, use this feedback to improve your project before submission.


Group Assessment Criteria

  1. The key points relating to the topic that your group allocated are clearly described.
  2. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
  3. Content is correctly cited and referenced.
  4. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
  5. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
  6. Relates the topic and content of the Wiki entry to learning aims of cell biology.
  7. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
  8. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
  9. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
  10. Develops and edits the wiki entries in accordance with the above guidelines.

Group 3: Z3463637 | Z3374116 | Z3332339 | Z5050826

Hey there guys, there are some interesting subtopics that we can discuss under the topic 'Extracellular Matrix'. Theres quite a bit of information on the different fibre types in the ECM as well as the mechanical properties of ECM. I think we should do a bit of simple research first and find which one we can find the most easily accessible information as well as which one seems more interesting to you guys. --Z3374116 (talk) 23:03, 22 March 2015 (EST)

--Z3463637 (talk) 11:21, 23 March 2015 (EST) Collagen would be a pretty easy topic to do! Theres quite a bit of information about this. I can see another group after this as well though... we should decide soon.

--Z3463637 (talk) 21:45, 23 March 2015 (EST)-- Well, collagen has been taken now so... Elastin? Seems like the next best option. We need to decide asap before all the good ones get taken. Please reply!!!!!

--Z3374116 (talk) 01:03, 24 March 2015 (EST) Elastin seems nice and simple!!

--Z3463637 (talk) 08:48, 24 March 2015 (EST) Elastin it is! I changed the heading for our page, before another group takes it. We can always change it if the other 2 members disagree.

--Z3332339 (talk) 09:18, 25 March 2015 (EST) Hey guys I think Elastin is a great topic to do! I think there is lots of info on it as well

--Z3463637 (talk) 18:01, 26 March 2015 (EST) IDEAS FOR SUBHEADINGS:

- Introduction

- History

- Structure - z3463637

- Function - z3332339

- Assembly -z3374116

- Clinical Significance [Does this cover the mutations?] - z5050826


Here are the subheadings we have so far, feel free to add more to this list and pick one to research.

--Z3463637 (talk) 21:00, 27 March 2015 (EST) Yes, clinical significance will include abnormalities/diseases associated with elastin.


Lab 3

In silico analysis suggests interaction between Ebola virus and the extracellular matrix. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333865/ <pubmed>PMC4333865</pubmed>

Matrix metalloproteinases in destructive lung disease. http://www.sciencedirect.com/science/article/pii/S0945053X15000396

Thrombosis, like other cardiovascular diseases, has a strong genetic component, with largely unknown determinants. EMILIN2, Elastin Microfibril Interface Located Protein2, was identified as a candidate gene for thrombosis in mouse and human quantitative trait loci studies. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319747/ <pubmed>PMC4319747</pubmed>

Vitamin a deficiency and alterations in the extracellular matrix. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245576/ <pubmed>PMC4245576</pubmed>

--z3374116 (talk) 15:28, 20 April 2015 (EST)

ASSEMBLY OF ELASTIN FIBRES

Article 1

Research article breaks down the main components of Elastin describing its characteristics. Observations on the alternating hydrophobic and cross-linking characteristics within Elastin are described. Focuses on explaining in depth the basis of self-organizational ability of elastin-based polypeptides and how the information from them can possibly assist in developing self-assembling biomaterials.

<pubmed>11911775</pubmed>

Article 2


<pubmed>25811498</pubmed>

Article 3

This article focuses on investigating the mechanisms behind the elastic fiber assembly via observing the molecular interactions between 'elastin' and 'microfibrillar' components using solid-phase binding assays. Observations found that the major cross-linking region in elastin is formed by association of domains encoded by exons 10.19 and 25 of tropoelastin.

<pubmed>8575256</pubmed>

Article 4

<pubmed>1648323</pubmed>

Article 5

<pubmed>18228265</pubmed>

--Z3463637 (talk) 11:18, 2 April 2015 (EST)

Article 1

The study highlights the role of recently identified glycoproteins on or surrounding elastic fibers of tissues and organs. Short fibulins, particularly -3, -4 and -5 have very potent elastogenic activities. The activities of these short fibulins were observed in knockout mice and it was found that they play an integral role in the formation of elastic fibers. This review article is relevant to the formation and structure of elastin.

<pubmed>PMC2880191</pubmed>

Article 2

Before manufacturing biomaterials, the biopolymer materials must be assessed based on their functionality and design parameters. The review article discusses the different biopolymer materials, including elastin, highlighting its structure and functional properties. It is found that a integrated design approach using experimental and computational modeling procedures are required to study these materials.

<pubmed>22938765</pubmed>

Article 3

Elastin is necessary for the elasticity and recoiling properties that are evident in blood vessels. In a study of elastin knockout mice, it was revealed that elastin also plays a role in arterial morphogenesis. This review article is relevant to the sub-topic as it highlights the functions and structure of elastin.

<pubmed>24679588</pubmed>

Article 4

Previous studies have shown that developing arteries and neural crest EGF-like (DANCE) proteins are essential for the formation of elastin. The identification of latent transforming growth factor-β-binding protein 2 (LTBP-2), was found to promote the deposition of DANCE proteins onto fibrillin-1 microfibrils. This ultimately induces the formation of elastin.

<pubmed>17581631</pubmed>

--Z3332339 (talk) 22:49, 26 April 2015 (EST)

Lab 3 Assessment


Changes in the structure-function relationship of elastin and its impact on the proximal pulmonary arterial mechanics of hypertensive calves [1]

Elastin is commonly found in the arteries due to the high pressure of blood coming from the heart, and its function to supply oxygenated blood to all organs, its elasticity is important due to enormous pressure it needs to withstand. Pulmonary arterial hypertension (PAH) causes stiffness in these arteries affecting the ability for these arteries to stretch and maintain a relatively constant pressure with high blood flow. This article looks at the structure and function of this relationship in PAH and the mechanobiological adaptations that are undergone by elastic arteries in response to PAH.


Comparison between human fetal and adult skin [2]

Elastin is important in adults for restoring backing the normal tissue architecture example pinching of skin. According to this research, extracellular components such as elastin are important in the scarless healing process that takes places in on early fetal gestation. The role that elastin or another name, tropoelastin is investigated. Elastin is not found in fetal skin up till week 22. Although it is not a primary extracellular component for scarless healing in fetal wounds, it is still plays a role in skin regeneration. A comparison between fetal skin and adult skin is looked at.


Spatial Distribution and Mechanical Function of Elastin in Resistance Arteries A Role in Bearing Longitudinal Stress [3]

Arteries within the human consists of three layers and are most evident closest to the heart due to the properties that make them withhold the enormous pressure the heart pumps. The walls of the arteries exist in three layers where the outermost layer, called the tunica adventitia providing tensile strength, the hypothesis this research grouped investigated was whether the elastin fibres are subject to longitudinal stretch.


Tropoelastin - a versatile, bioactive assembly module [4]

The monomer for elastin is tropoelastin. Thus when many Tropoelastin molecules are bonded together (covalent bonds )with cross links such as lysal oxidase [5] they bind together to form the protein elastin. There is only one gene for that codes for tropoelastin and thus only one protein. The research stems on the facts that tropoelastin is compatible with synthetic and natural co-polymers. As a result of this, it enables these researches to expand upon the applications of its potential use in next-generation tailored bioactive materials such as when responding to injury. this is because large quantities of the monomer have only become accessible recently. Isolation of tropoelastin was previously intricate and inefficient due to its rate of cross linking incorporated into growing the elastic fiber in the living. However by synthesizing the elastin gene, this has allowed for a recombinant tropoelastin that is identical to the naturally secreted human form giving that compatibility that allows scientist and research to work with in a versatile way.


Insights into the role of elastin in vocal fold health and disease [6]

Elastin can be defined as a an extracellular matrix protein that is responsible for tissue elastic recoil. Therefore, because of its function it can then be assumed that it is found in different parts of the human body that require tissue recoiling. For example, lungs(30%)- expansion when inhaling and exhaling air, large arteries (70%)-to be able to recoil back to their shape consistently as blood is bumped through them, skin (2-4%)- to be able to withstand stretches and sustain its original shape. Elastin can also be found in the vocal fold of the lamina propria, making up 9% of the total protein. Thus, the lamina propria experiences greater amounts of mechanical strain relative to skin but less when compared to lungs and arteries.


--Z3463637 (talk) 20:35, 11 April 2015 (EST) I will also be talking about distribution in the "structure" subheading.

--Z3463637 (talk) 00:26, 23 April 2015 (EST) I've started working on the introduction, and i'll include the distribution here instead. Feel free to change or add anything to the introduction! How is everyone going with their sections? Also, I haven't found much about the history of elastin in my research - maybe we can exclude or replace this heading if we can't find much information.

--Z3374116 (talk) 15:46, 23 April 2015 (EST) Ill try help search for historical info as well (I think it might be good to even have a little bit of historical information). We could add more subheadings as seem fit provided we can finish our sections :p. Been searching for my part finding some good stuff, just need to slowly paste stuff into our wikipage. The weather and blackouts havent helped much. Lets slowly get things rolling.

Thanks for adding the intro

--Z3374116 (talk) 18:07, 23 April 2015 (EST) Mark said that because we have such a specialized structure, we need to know everything about all things related.Interactions, genome etc.

Pubmed Protein Database / Pubmed Genome Database


--Z3332339 (talk) 22:55, 26 April 2015 (EST) Ok i had my section on just the wiki page but not here on discussion but i just pasted it here as well just in case because i reread the assessment item and it said discussion page. Thanks for adding the intro :) I remember him saying those things too and i remember him saying include any isoforms of it, I also remember him mentioning the word Mendelian ..but im not sure what he means by this.. do you guys recall?


--Z3332339 (talk) 17:54, 30 April 2015 (EST) Hey guys so from todays feedback from Mark we need to look at

-expression

-how its made

-gene that codes for it

-knock out mouse database

-fibre (aus one)/fiber- and write this in intro

-degradation

-turn over of elastin--arteries, get stiff

-distribution-tunica intima and tunica externa

-components of elastic fiber

-interaction

-organs

-histological images


--Z3463637 (talk) 13:04, 3 May 2015 (EST) I moved the component's subheadings under structure.

Heres the intro you wrote under components z3374116 - I think it should go under the "Assembly" heading. I understand how closely related these two sections are, but i will try to talk about the interactions of the componenets only very briefly, without talking about assembly too much.

The formation of Elastic fibers is a complex process which is comprised of multiple interactions between different proteins, enyzmes and binding sites. Elastic fibers are mostly comprised of Elastin and Micro-fibrils [7] which consist make up most of the structure, the processes of formation are closely regulated and carried out by the smaller protein components; Tropoelastin, Lysyl Oxidases (LOX), Fibrilin-4 / Fibrilin-5 and Integrins


--Z3332339 (talk) 09:39, 4 May 2015 (EST)

He also said Histological diagrams, so i added it to the list :)... he also said he could give us some too :P

--Z3374116 (talk) 16:11, 4 May 2015 (EST)

Mark also told us to include hand drawn diagrams it to our project, Im currently drawing mine out (assembly steps). Lets try sort all this out by this week Thursday because peer assesments are coming up!

  1. <pubmed>PMC2593497</pubmed>
  2. <pubmed>PMC2799629</pubmed>
  3. <pubmed>PMC3380608</pubmed>
  4. <pubmed>PMC3879170</pubmed>
  5. <pubmed>PMC3190022</pubmed>
  6. <pubmed>PMC3190022</pubmed>
  7. <pubmed>18228265</pubmed>